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Detection of coproantibodies and faecal immune complexes in human trichinellosis

Published online by Cambridge University Press:  11 December 2006

G. G. NUÑEZ ,
S. N. COSTANTINO  and
S. M. VENTURIELLO
G. G. NUÑEZ
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina-“Prof. Dr. R. A. Margni” Humoral Immunity Studies Institute, CONICET. Junín 956, (1113) Buenos Aires, Argentina
S. N. COSTANTINO
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina-“Prof. Dr. R. A. Margni” Humoral Immunity Studies Institute, CONICET. Junín 956, (1113) Buenos Aires, Argentina
S. M. VENTURIELLO*
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina-“Prof. Dr. R. A. Margni” Humoral Immunity Studies Institute, CONICET. Junín 956, (1113) Buenos Aires, Argentina
*
*Corresponding author: Cátedra de Inmunología, Departamento de Microbiología, Inmunología y Biotecnología, Facultad de Farmacia y Bioquímica, Junín 956, (1113) Buenos Aires, Argentina. Tel: +54 11 4964 8259. Fax: +5411 4964 0024. E-mail: sventuri@huemul.ffyb.uba.ar
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Summary

Trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. Even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. Based on the immune response generated by the host's intestinal mucosa, the aim of this work was to develop ELISAs to assess the presence of coproantigens (CAgs), coproantibodies (CAbs) and faecal immune complexes in stool samples of 18 individuals belonging to different outbreaks that have arisen in Argentina. By the methodologies developed in this work it was found that anti-muscle larva excretory-secretory products (ML-ESP) CAbs were detected in 89% of the samples analysed regardless of the time p.i. Anti-ML-ESP IgA, IgG, IgE and IgM were detected in 56%, 56%, 28% and 22% of the individuals respectively. Those samples negative for anti-ML-ESP total immunoglobulins proved positive for anti-adult worm-ESP CAbs. No CAgs were detected in any of the samples. The results obtained in this work indicate that the intestinal immune response in human trichinellosis is featured by all the isotypes of specific immunoglobulins. Furthermore, the detection of antibodies in stool samples, in either the free or complexed form, could be applied to confirm early human trichinellosis.

Keywords

human trichinellosiscoproantibodiescoproantigensfaecal immune complexesimmunoglobulin isotypes
Type
Research Article
Information
Parasitology , Volume 134 , Issue 5 , May 2006 , pp. 723 - 727
Copyright
Copyright © Cambridge University Press 2006

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