Hostname: page-component-848d4c4894-x24gv Total loading time: 0 Render date: 2024-05-17T09:44:48.953Z Has data issue: false hasContentIssue false
  • English
  • Français

Elevated levels of urinary hydrogen peroxide, advanced oxidative protein product (AOPP) and malondialdehyde in humans infected with intestinal parasites

Published online by Cambridge University Press:  21 January 2009

S. CHANDRAMATHI ,
K. SURESH ,
Z. B. ANITA  and
U. R. KUPPUSAMY
S. CHANDRAMATHI
Affiliation:
Department of Parasitology, University Malaya, Kuala Lumpur, Malaysia
K. SURESH*
Affiliation:
Department of Parasitology, University Malaya, Kuala Lumpur, Malaysia
Z. B. ANITA
Affiliation:
Unit of Clinical Oncology, University Malaya, Kuala Lumpur, Malaysia
U. R. KUPPUSAMY
Affiliation:
Department of Molecular Medicine, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia
*
*Corresponding author: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603Kuala Lumpur, Malaysia. Tel: +603 79674743. Fax: +603 79674754. E-mail: suresh@um.edu.my
Get access Rights & Permissions [Opens in a new window]

Summary

Oxidative stress has been implicated as an important pathogenic factor in the pathophysiology of various life-threatening diseases such as cancer, cardiovascular diseases and diabetes. It occurs when the production of free radicals (generated during aerobic metabolism, inflammation, and infections) overcome the antioxidant defences in the body. Although previous studies have implied that oxidative stress is present in serum of patients with parasitic infection there have been no studies confirming oxidative stress levels in the Malaysian population infected with intestinal parasites. Three biochemical assays namely hydrogen peroxide (H2O2), lipid peroxidation (LP) and advanced oxidative protein product (AOPP) assays were carried out to measure oxidative stress levels in the urine of human subjects whose stools were infected with parasites such as Blastocystis hominis, Ascaris, Trichuris, hookworm and microsporidia. The levels of H2O2, AOPP and LP were significantly higher (P<0·001, P<0·05 and P<0·05 respectively) in the parasite-infected subjects (n=75) compared to the controls (n=95). In conclusion, the study provides evidence that oxidative stress is elevated in humans infected by intestinal parasites. This study may influence future researchers to consider free radical-related pathways to be a target in the interventions of new drugs against parasitic infection and related diseases.

Keywords

intestinal parasitic infectionoxidative stressoxidative indices
Type
Research Article
Information
Parasitology , Volume 136 , Issue 3 , March 2009 , pp. 359 - 363
Copyright
Copyright © 2009 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Banerjee, D., Madhusoodanan, U. K., Nayak, S. and Jacob, J. (2003). Urinary hydrogen peroxide: a probable marker of oxidative stress in malignancy. Clinica Chimica Acta 334, 205–209.CrossRefGoogle ScholarPubMed
Banerjee, D., Kumar, A. P., Kumar, B., Madhusoodanan, U. K., Nayak, S. and Jacob, J. (2002). Determination of absolute hydrogen peroxide concentration by spectrophotometric method. Current Science 83, 1193–1194.Google Scholar
Chandramathi, S., Suresh, K., Anita, Z. B. and Kuppusamy, U. R. (2008). Comparative assessment of urinary oxidative indices in breast and colorectal cancer patients. Journal of Cancer Research and Clinical Oncology (Epub ahead of print.).Google ScholarPubMed
Chikibova, G. I. and Sanikidze, T. V. (2006). The role of oxygen-nitrogen species in pathogenesis of amoebiasis. Georgian Medical News 131, 96–99.Google Scholar
Clark, I. A., Hunt, N. H. and Cowden, B. W. (1986). Oxygen-derived free radical in the pathogenesis of parasitic disease. Advances in Parasitology 25, 1–28.CrossRefGoogle ScholarPubMed
Dermici, M., Delibas, N., Altuntas, I., Oktem, F. and Yonden, Z. (2003). Serum iron, zinc and copper levels and lipid peroxidation in children with chronic giardiasis. Journal of Health, Population, and Nutrition 21, 72–75.Google Scholar
Derda, M., Wandurska-Nowak, E. and Hadas, E. (2004). Changes in the level of antioxidants in the blood from mice infected with Trichinella spiralis. Parasitology Research 93, 207–210.CrossRefGoogle ScholarPubMed
Halliwell, B. (1994). Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 344, 721–724.CrossRefGoogle ScholarPubMed
Halliwell, B. (2000). Hydrogen peroxide in the human body. FEBS Letters 486 (1), 10–13.CrossRefGoogle ScholarPubMed
Kamalvand, G. and Ali-Khan, Z. (2004). Immunolocalization of lipid peroxidation/advanced glycation end products in amyloid A amyloidosis. Free Radical Biology & Medicine 36, 657–664.CrossRefGoogle ScholarPubMed
Kilic, E., Yazar, S. and Saraymen, R. (2003). Lipid peroxidation level in patients with Blastocystosis. Journal of Inonu University Medical Faculty 10, 1–3.Google Scholar
Kirschbaum, B. (2001). Total urine antioxidant capacity. Clinica Chimica Acta 305, 167–173.CrossRefGoogle ScholarPubMed
Kita, K., Hirawake, H., Miyadera, H., Amino, H. and Takeo, S. (2002). Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum. Biochimica et Biophysica Acta 1553, 123–139.CrossRefGoogle Scholar
Kalousova, M., Skrha, J. and Zima, T. (2002). Advanced glycation end-products and advanced oxidation protein products in patients with diabetes melitus. Physiological Research 51, 597–604.CrossRefGoogle Scholar
Long, H. L., Evans, J. P. and Halliwell, B. (1999). Hydrogen peroxide in human urine: implications for antioxidants defense and redox regulation. Biochemical and Biophysical Research Communications 262, 202–226.CrossRefGoogle ScholarPubMed
Nourooz-Zadeh, J. (1999). Ferrous ion oxidation in presence of xylenol orange for detection of lipid hydroperoxides in plasma. In Methods in Enzymology, Vol. 300 (ed. Packer, L., pp. 58–62. Academic Press, San Diego, USA.Google Scholar
Oliveira, F. J. and Cecchini, R. (2000). Oxidative stress of liver in hamsters with Leishmania (L.) chagasi. The Journal of Parasitology 86, 1067–1072.CrossRefGoogle ScholarPubMed
Oshima, H. and Bartsch, H. (1994). Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutation Research 305, 253–264.CrossRefGoogle Scholar
Ratty, A. and Das, N. P. (1986). Lipid peroxidation in the rat brain mitochondria in the presence of ascorbic acid. IRCS Medical Science 14, 815–816.Google Scholar
Rosen, G. M., Pou, S., Ramos, C. L., Cohen, M. S. and Britigan, B. E. (1995). Free radicals and phagocytic cells. The FASEB Journal 9, 200–209.CrossRefGoogle ScholarPubMed
Saygili, E. I., Konukoglu, D., Papila, C. and Akcay, T. (2003). Levels of plasma vitamin E, vitamin C, TBARS, and cholesterol in male patients with colorectal tumors. Biochemistry 68, 325–328.Google ScholarPubMed
Shioji, I. (2005). Oxidative stress related diseases and biopyrins. Rinsho Byori 53, 155–159.Google Scholar
Siciarz, A., Weinberger, B., Witz, G., Hiatt, M. and Hegyi, T. (2001). Urinary thiobarbituric acid-reacting substances as potential biomarkers of intrauterine hypoxia. Archives of Pediatrics and Adolescent Medicine 155, 718–722.CrossRefGoogle ScholarPubMed
Turrens, J. F. (2004). Oxidative stress and antioxidant defenses: a target for the treatment of diseases caused by parasitic protozoa. Molecular Aspects of Medicine 25, 211–220.CrossRefGoogle ScholarPubMed
Valko, M., Rhodes, C. J., Moncol, J., Izakovic, M. and Mazur, M. (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions 160, 1–40.CrossRefGoogle ScholarPubMed
Witko-Sarsat, V., Friedlander, M., Capeillere-Blandin, C., Nguyen-Khoa, T., Nguyen, A. H., Canterloup, S., Drayer, J. M., Jungers, P., Drueke, T. and Deschamps-Latscha, B. (1998). Advanced oxidation protein products as novel mediators of inflammation and monocyte activation in chronic renal failure. Journal of Immunology 16, 2524–2532.CrossRefGoogle Scholar
Yagi, K. (1994). Lipid peroxides in hepatic, gastrointestinal, and pancreatic diseases. Advances in Experimental Medicine and Biology 366, 165–169.CrossRefGoogle ScholarPubMed