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Evaluation of an anti-parasitic compound extracted from Streptomyces sp. HL-2-14 against fish parasite Ichthyophthirius multifiliis

Published online by Cambridge University Press:  24 March 2015

YUN-HANG GAO ,
XIAO-FENG SHAN ,
YUAN-HUAN KANG ,
YU-XUAN SHENG ,
LONG CHEN ,
WEI WANG  and
HONG-XIA MA
YUN-HANG GAO
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China
XIAO-FENG SHAN
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China
YUAN-HUAN KANG
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China
YU-XUAN SHENG
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China
LONG CHEN
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China
WEI WANG
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China
HONG-XIA MA*
Affiliation:
Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China
*
*Corresponding author. College of life science, Jilin Agriculture University, 2888 Xincheng Road, Changchun 130118, Jilin, China. E-mail: jilinau2@126.com
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Summary

The present study was conducted to evaluate the anti-parasitic activity of a pure compound from Streptomyces sp. HL-2-14 against fish parasite Ichthyophthirius multifiliis, and elucidate its chemical structure. By electron ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectrum (1H NMR and 13C NMR), the compound was identified as amphotericin B (AmB). The in vitro trials revealed that AmB can effectively kill the theronts and tomonts of I. multifiliis with the median lethal concentration (LC50) of 0·8 mg L−1 at 30 min for the theronts and 4·3 mg L−1 at 2 h for the tomonts, respectively. AmB at 5 mg L−1 significantly reduced I. multifiliis infectivity prevalence and intensity on grass carp (Ctenopharyngodon idella), and consequently decreased fish mortality, from 100% in control group to 30% in treated group. The 72 h acute toxicity (LC50) of AmB on grass carp was 20·6 mg L−1, but fish mortality was occurred when exposure to 13·0 mg L−1. These results indicated that AmB was effective in the therapy of I. multifiliis infection, but the safety concentration margin is relatively narrow. Further efforts aiming to decrease the toxicity and improve the therapeutic profile remain to be needed.

Keywords

ichthyophthiriasisstreptomyceteamphotericin Banti-parasiticsgrass carp
Type
Research Article
Information
Parasitology , Volume 142 , Issue 7 , June 2015 , pp. 910 - 916
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Copyright
Copyright © Cambridge University Press 2015 

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