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First report of Trypanosoma dionisii (Trypanosomatidae) identified in Australia

Published online by Cambridge University Press:  28 September 2020

Jill M. Austen Open the ORCID record for Jill M. Austen [Opens in a new window] ,
Esther Van Kampen ,
Siobhon L. Egan ,
Mark A. O'Dea ,
Bethany Jackson ,
Una M. Ryan ,
Peter J. Irwin  and
Diana Prada
Jill M. Austen*
Affiliation:
Vector and Waterborne Pathogens Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth6150, Australia
Esther Van Kampen
Affiliation:
School of Veterinary Medicine, Murdoch University, Perth, WA6150, Australia
Siobhon L. Egan
Affiliation:
Vector and Waterborne Pathogens Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth6150, Australia
Mark A. O'Dea
Affiliation:
School of Veterinary Medicine, Murdoch University, Perth, WA6150, Australia
Bethany Jackson
Affiliation:
School of Veterinary Medicine, Murdoch University, Perth, WA6150, Australia
Una M. Ryan
Affiliation:
Vector and Waterborne Pathogens Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth6150, Australia
Peter J. Irwin
Affiliation:
Vector and Waterborne Pathogens Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth6150, Australia
Diana Prada
Affiliation:
School of Veterinary Medicine, Murdoch University, Perth, WA6150, Australia
*
Author for correspondence: Jill M. Austen, E-mail: J.Austen@murdoch.edu.au
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Abstract

Trypanosomes are blood-borne parasites that can infect a variety of different vertebrates, including animals and humans. This study aims to broaden scientific knowledge about the presence and biodiversity of trypanosomes in Australian bats. Molecular and morphological analysis was performed on 86 blood samples collected from seven different species of microbats in Western Australia. Phylogenetic analysis on 18S rDNA and glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) sequences identified Trypanosoma dionisii in five different Australian native species of microbats; Chalinolobus gouldii, Chalinolobus morio, Nyctophilus geoffroyi, Nyctophilus major and Scotorepens balstoni. In addition, two novels, genetically distinct T. dionisii genotypes were detected and named T. dionisii genotype Aus 1 and T. dionisii genotype Aus 2. Genotype Aus 2 was the most prevalent and infected 20.9% (18/86) of bats in the present study, while genotype Aus 1 was less prevalent and was identified in 5.8% (5/86) of Australian bats. Morphological analysis was conducted on trypomastigotes identified in blood films, with morphological parameters consistent with trypanosome species in the subgenus Schizotrypanum. This is the first report of T. dionisii in Australia and in Australian native bats, which further contributes to the global distribution of this cosmopolitan bat trypanosome.

Keywords

AustraliaChalinolobus gouldiiChalinolobus moriomicrobatsNyctophilus geoffroyiNyctophilus majorScotorepens balstoniTrypanosoma dionisii
Type
Research Article
Information
Parasitology , Volume 147 , Issue 14 , December 2020 , pp. 1801 - 1809
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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