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Genetic diversity and evolution of Pneumocystis fungi infecting wild Southeast Asian murid rodents

Published online by Cambridge University Press:  09 November 2017

Alice Latinne ,
François Bezé ,
Laurence Delhaes ,
Muriel Pottier ,
Nausicaa Gantois ,
Julien Nguyen ,
Kim Blasdell ,
Eduardo Dei-Cas ,
Serge Morand  and
Magali Chabé
Alice Latinne*
Affiliation:
Institut des Sciences de l'Evolution (ISEM), UMR 5554 CNRS-IRD-UM2, CC65, Université de Montpellier 2, Montpellier, France Departement of Life Sciences-Conservation Genetics, University of Liège, Liège, Belgium EcoHealth Alliance, New York, USA
François Bezé
Affiliation:
Medical Laboratory, Dunkerque Hospital, Dunkerque, France Univ. Lille, CNRS, Inserm, CHU de Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Center for Infection and Immunity of Lille, Lille, France
Laurence Delhaes
Affiliation:
Univ. Lille, CNRS, Inserm, CHU de Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Center for Infection and Immunity of Lille, Lille, France Service de Parasitologie-Mycologie – ISERM U1045, CHU de Bordeaux, Université de Bordeaux, France
Muriel Pottier
Affiliation:
Univ. Lille, CNRS, Inserm, CHU de Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Center for Infection and Immunity of Lille, Lille, France
Nausicaa Gantois
Affiliation:
Univ. Lille, CNRS, Inserm, CHU de Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Center for Infection and Immunity of Lille, Lille, France
Julien Nguyen
Affiliation:
Univ. Lille, CNRS, Inserm, CHU de Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Center for Infection and Immunity of Lille, Lille, France
Kim Blasdell
Affiliation:
CSIRO Health and Biosecurity Business Unit, Australian Animal Health Laboratory, Geelong, Australia
Eduardo Dei-Cas
Affiliation:
Univ. Lille, CNRS, Inserm, CHU de Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Center for Infection and Immunity of Lille, Lille, France
Serge Morand
Affiliation:
Institut des Sciences de l'Evolution (ISEM), UMR 5554 CNRS-IRD-UM2, CC65, Université de Montpellier 2, Montpellier, France CNRS-CIRAD, Centre Infectiologie Christophe Mérieux du Laos, Vientiane, LAOS PDR
Magali Chabé
Affiliation:
Univ. Lille, CNRS, Inserm, CHU de Lille, Institut Pasteur de Lille, U1019 – UMR 8204 – CIIL – Center for Infection and Immunity of Lille, Lille, France
*
Author for correspondence: Alice Latinne, E-mail: latinne@ecohealthalliance.org
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Abstract

Pneumocystis organisms are airborne-transmitted fungal parasites that infect the lungs of numerous mammalian species with strong host specificity. In this study, we investigated the genetic diversity and host specificity of Pneumocystis organisms infecting Southeast Asian murid rodents through PCR amplification of two mitochondrial genes and tested the co-phylogeny hypothesis among these fungi and their rodent hosts. Pneumocystis DNA was detected in 215 of 445 wild rodents belonging to 18 Southeast Asian murid species. Three of the Pneumocystis lineages retrieved in our phylogenetic trees correspond to known Pneumocystis species, but some of the remaining lineages may correspond to new undescribed species. Most of these Pneumocystis species infect several rodent species or genera and some sequence types are shared among several host species and genera. These results indicated a weaker host specificity of Pneumocystis species infecting rodents than previously thought. Our co-phylogenetic analyses revealed a complex evolutionary history among Pneumocystis and their rodent hosts. Even if a significant global signal of co-speciation has been detected, co-speciation alone is not sufficient to explain the observed co-phylogenetic pattern and several host switches are inferred. These findings conflict with the traditional view of a prolonged process of co-evolution and co-speciation of Pneumocystis and their hosts.

Keywords

PneumocystisMurid rodentsRattinico-phylogenyco-speciationSoutheast Asia
Type
Research Article
Information
Parasitology , Volume 145 , Issue 7 , June 2018 , pp. 885 - 900
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Copyright
Copyright © Cambridge University Press 2017 

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