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Genetic structure of Anisakis physeteris, and its differentiation from the Anisakis simplex complex (Ascaridida: Anisakidae)

Published online by Cambridge University Press:  06 April 2009

S. Mattiucci ,
G. Nascetti ,
L. Bullini ,
P. Orecchia  and
L. Paggi
S. Mattiucci
Affiliation:
Institute of Parasitology, University of Rome ‘La Sapienza’, P. le Aldo Moro 5, 00185 Rome, Italy
G. Nascetti
Affiliation:
Department of Genetics and Molecular Biology, University of Rome ‘La Sapienza’, Via Lancisi 29, 00161 Rome, Italy
L. Bullini
Affiliation:
Department of Genetics and Molecular Biology, University of Rome ‘La Sapienza’, Via Lancisi 29, 00161 Rome, Italy
P. Orecchia
Affiliation:
Institute of Parasitology, University of Rome ‘La Sapienza’, P. le Aldo Moro 5, 00185 Rome, Italy
L. Paggi
Affiliation:
Institute of Parasitology, University of Rome ‘La Sapienza’, P. le Aldo Moro 5, 00185 Rome, Italy
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Summary

The genetic structure of Anisakis physeteris from the Mediterranean Sea has been analysed electrophoretically at 22 enzyme loci. The samples studied, although differing in the life-stage (larvae and adults), and in the host (the fishes Micromesistius poutassou and Trachurus trachurus, and the sperm whale Physeter macrocephalus) were genetically homogeneous. Of these loci 11 (Ldh, Sod, Np, Adk-2, Pgm-1, Est-1, Est-2, Acph-1, Acph-2, Lap-2 and Ca) were found to be monomorphic, while the other 11 (Sdh, Mdh, ldh, 6-Pgdh, G3pdh, Got, Adk-1, Pgm-2, Lap-1, Mpi and Gpi) showed from 2 to 7 alleles. The following values of genetic variability were estimated: He = 0·ll, P = 0·50, A = 1·95. Distinct alleles were found between A. physeteris and the A. simplex complex at 19 out of the 22 loci studied, and only few rare alleles were shared at the remaining 3 loci. The genetic divergence between A. physeteris and A. simplex A and B is therefore very high, the values of Nei’s index D being 7·384 and 6·443 respectively (I = 0·001 and 0·002). The assignation of A. physeteris and the A. simplex complex to two distinct subgenera, Skrjabinisakis and Anisakis, as proposed by Mosgovoy on a morphological basis, appears to be fully justified according to our genetic data.

Type
Research Article
Information
Parasitology , Volume 93 , Issue 2 , October 1986 , pp. 383 - 387
Copyright
Copyright © Cambridge University Press 1986

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