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Hymenolepis diminuta: changes in intestinal morphology and the enterochromaffin cell population associated with infection in male C57 mice

Published online by Cambridge University Press:  06 April 2009

D. M. Mckay ,
D. W. Halton ,
C. F. Johnston ,
I. Fairweather  and
C. Shaw
D. M. Mckay
Affiliation:
School of Biology and Biochemistry, The Queen's University, Belfast BT7 1NN, U.K.
D. W. Halton
Affiliation:
School of Biology and Biochemistry, The Queen's University, Belfast BT7 1NN, U.K.
C. F. Johnston
Affiliation:
Department of Medicine, The Queen's University, Belfast BT7 1NN, U.K.
I. Fairweather
Affiliation:
School of Biology and Biochemistry, The Queen's University, Belfast BT7 1NN, U.K.
C. Shaw
Affiliation:
Department of Medicine, The Queen's University, Belfast BT7 1NN, U.K.
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Summary

Mean villus height, crypt depth and the number of 5-HT-positive enterochromaffin (EC) cells have been examined in two regions of the small intestine (20–30° and 60–70° distance from the pylorus) of male, 6 to 8-week-old, C57 mice following a 5-cysticercoid infection of the rat tapeworm, Hymenolepis diminuta. Test mice and sham-infected controls were autopsied 0, 4, 8, 10, 14 and 28 days post-primary infection (p-1°-i) and 2, 4, 5, 7 and 14 days post-secondary infection (p-2°-i), administered 28 days p-1°-i. Morphometric analysis revealed a statistically significant increase in crypt depth in the 60–70°o intestine region in infected mice during both primary and secondary infections; no significant deviation from the control was observed for villus height in infected mice. Statistical analysis showed a significant increase in the number of 5-HT-positive EC cells in infected mice. This response occurred in the lower portion of the intestine on days 10-p-1°-i and 5-p-2°-i, and was not due to increased mucosal surface area in this region. Results are discussed with reference to murine cestode rejection and the possible involvement therein of the neuroendocrine system.

Keywords

Hymenolepis diminutacestodeC57 mouseintestinal morphometricsvillus height/crypt depthenterochromaffin cell5-hydroxytryptamine
Type
Research Article
Information
Parasitology , Volume 101 , Issue 1 , August 1990 , pp. 107 - 113
Copyright
Copyright © Cambridge University Press 1990

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