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Identification and characterization of a cDNA clone-encoding antigen of Eimeria acervulina

Published online by Cambridge University Press:  21 August 2012

HUILI ZHU ,
LIXIN XU ,
RUOFENG YAN ,
XIAOKAI SONG ,
FANG TANG ,
SONG WANG  and
XIANGRUI LI
HUILI ZHU
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
LIXIN XU
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
RUOFENG YAN
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
XIAOKAI SONG
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
FANG TANG
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
SONG WANG
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
XIANGRUI LI*
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China
*
*Corresponding author: Tel: +86 25 84399000. Fax: +86 25 84399000. E-mail: lixiangrui@njau.edu.cn
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Summary

Eimeria spp. are the causative agents of coccidiosis, a major disease affecting the poultry industry. So far, only a few antigen genes of E. acervulina have been reported. In this study, a clone, named as cSZ-JN2, was identified from a cDNA expression library prepared from E. acervulina sporozoite stage with the ability to stimulate the chicken immune response. The sequence analysis showed that the open reading fragment (ORF) of cSZ-JN2 was 153 bp in size and encoded a predicted protein of 50 amino acids of Mr 5·3 kDa. BLASTN search revealed that cSZ-JN2 had no significant homology with the known genes of E. acervulina or any other organism (GenBank). The recombinant cSZ-JN2 antigen expressed in E. coli was recognized strongly by serum from chickens experimentally infected with E. acervulina. Immunofluorescence analysis using antibody against recombinant cSZ-JN2 indicated that this protein was expressed in sporozoite and merozoite developmental stages. Animal challenge experiments demonstrated that the recombinant protein of cSZ-JN2 and DNA vaccine carrying cSZ-JN2 could significantly increase the average body weight gains, decrease the mean lesion scores and the oocyst outputs of the immunized chickens and presented anti-coccidial indices of more than 165. All the above results suggested that the cSZ-JN2 was a novel E. acervulina antigen and could be an effective candidate for the development of a new vaccine against E. acervulina infection.

Keywords

cSZ-JN2Eimeria acervulinaantigencDNA expression library
Type
Research Article
Information
Parasitology , Volume 139 , Issue 13 , November 2012 , pp. 1711 - 1719
Copyright
Copyright © Cambridge University Press 2012

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