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Identification of a Nosema bombycis (Microsporidia) spore wall protein corresponding to spore phagocytosis

Published online by Cambridge University Press:  15 July 2011

SHUNFENG CAI ,
XINGMENG LU ,
HAIHONG QIU ,
MINGQIAN LI  and
ZHENZHEN FENG
SHUNFENG CAI
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
XINGMENG LU*
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
HAIHONG QIU
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
MINGQIAN LI
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
ZHENZHEN FENG
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author: Fax: +86 571 86971697. E-mail: xmlu@zju.edu.cn
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Summary

Life-cycle stages of the microsporidia Nosema bombycis, the pathogen causing silkworm pebrine, were separated and purified by an improved method of Percoll-gradient centrifugation. Soluble protein fractions of late sporoblasts (spore precursor cells) and mature spores were analysed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Protein spots were recovered from gels and analysed by mass spectrometry. The most abundant differential protein spot was identified by database search to be a hypothetical spore wall protein. Using immunoelectron microscopy, we demonstrated that HSWP5 is localized to the exospore of mature spores and renamed it as spore wall protein 5 (NbSWP5). Further spore phagocytosis assays indicated that NbSWP5 can protect spores from phagocytic uptake by cultured insect cells. This spore wall protein may function both for structural integrity and in modulating host cell invasion.

Keywords

MicrosporidiaNosema bombycis2D-PAGEspore wall proteinsporoblastphagocytosis
Type
Research Article
Information
Parasitology , Volume 138 , Issue 9 , August 2011 , pp. 1102 - 1109
Copyright
Copyright © Cambridge University Press 2011

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