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Identification of Leishmania infantum in blood donors from endemic regions for visceral leishmaniasis

Published online by Cambridge University Press:  04 November 2020

Loren Queli Pereira Open the ORCID record for Loren Queli Pereira [Opens in a new window] ,
Márcia Maria Ferreira-Silva Open the ORCID record for Márcia Maria Ferreira-Silva [Opens in a new window] ,
Cristhianne Molinero Andrade Ratkevicius Open the ORCID record for Cristhianne Molinero Andrade Ratkevicius [Opens in a new window] ,
César Gómez-Hérnandez Open the ORCID record for César Gómez-Hérnandez [Opens in a new window] ,
Fernanda Bernadelli De Vito Open the ORCID record for Fernanda Bernadelli De Vito [Opens in a new window] ,
Sarah Cristina Sato Vaz Tanaka Open the ORCID record for Sarah Cristina Sato Vaz Tanaka [Opens in a new window] ,
Virmondes Rodrigues Júnior Open the ORCID record for Virmondes Rodrigues Júnior [Opens in a new window]  and
Helio Moraes-Souza Open the ORCID record for Helio Moraes-Souza [Opens in a new window]
Loren Queli Pereira*
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Márcia Maria Ferreira-Silva
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Cristhianne Molinero Andrade Ratkevicius
Affiliation:
Laboratory of Immunology, Triângulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil
César Gómez-Hérnandez
Affiliation:
Laboratory of Immunology, Triângulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil
Fernanda Bernadelli De Vito
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Sarah Cristina Sato Vaz Tanaka
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
Virmondes Rodrigues Júnior
Affiliation:
Laboratory of Immunology, Triângulo Mineiro Federal University, Uberaba, Minas Gerais, Brazil
Helio Moraes-Souza
Affiliation:
Laboratory of Hematological Research of the Triângulo Mineiro Federal University and Uberaba Regional Blood Center – Hemominas Fundation, Uberaba, Minas Gerais, Brazil
*
Author for correspondence: Helio Moraes-Souza, E-mail: helio.moraes@uftm.edu.br
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Abstract

Visceral leishmaniasis is an endemic protozoonosis observed in over 60 countries, with over 500 000 new cases recorded annually. Although the diagnostic procedure of its symptomatic forms is well established, for asymptomatic patients, who represent about 85% of those infected, there is no consensus on the best method for its identification. Recent studies have presented molecular techniques as viable identification methods, with good sensitivity and specificity indices in asymptomatic individuals. Therefore, we aimed to use molecular methods to assess their effectiveness in identifying the presence of asymptomatic infection by Leishmania infantum (L. infantum) individuals from endemic regions of Brazil. Screening was performed by real-time polymerase chain reaction (qPCR) and confirmed by sequencing the cytochrome B gene. Of the 127 samples [from 608 blood donors who had participated in a previous study, of which 34 were positive by the enzyme-linked immunosorbent assay (ELISA) rK39] tested by qPCR, 31 (24.4%) were positive. In the sequencing of 10 qPCR-positive samples, five were identified as L. infantum. Complimentary samples of the ELISA rK39 and conventional PCR showed only reasonable and low agreement with qPCR, respectively. The qPCR confirmed the presence of infection in five of the 10 sequenced samples, ELISA confirmed three, and the conventional PCR confirmed none.

Keywords

Asymptomatic visceral leishmaniasisblood donorsCytBLeishmania infantumqPCR
Type
Research Article
Information
Parasitology , Volume 148 , Issue 1 , January 2021 , pp. 110 - 114
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Alvar, J, Vélez, ID, Bern, C, Herrero, M, Desjeux, P, Cano, J, Jannin, J and Boer, MD (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7, 1–12.CrossRefGoogle ScholarPubMed
Alvar, J, Alves, F, Bucheton, B, Burrows, L, Büscher, F, Carrillo, E, Felger, I, Hübner, MP, Moreno, J, Pinazo, MJ, Ribeiro, I, Sosa-Estani, S, Specht, S, Tarral, A, Wourgaft, NS and Bilbe, G (2020) Implications of asymptomatic infection for the natural history of selected parasitic tropical diseases. Seminars in Immunopathology 42, 231–246.CrossRefGoogle ScholarPubMed
Asato, Y, Oshiro, M, Myint, CK, Yamamoto, Y, Kato, H, Marco, JD, Mimori, T, Gomez, EAL, Hashiguchi, Y and Uezato, H (2009) Phylogenic analysis of the genus Leishmania by cytochrome b gene sequencing. Experimental Parasitology 121, 352–361.CrossRefGoogle ScholarPubMed
Cummins, D, Amin, S, Halil, O, Chiodini, PL, Hewitt, PE and Radley-Smith, R (1995) Visceral leishmaniasis after cardiac surgery. Archives of Disease in Childhood 72, 235–236.CrossRefGoogle ScholarPubMed
Ferreira-Silva, MM, Teixeira, LAS, Tibúrcio, MS, Pereira, GA, Santana, MP, Afonso, P, Alves, M, Feitosa, JM, Urias, E, Santos, EM, Carvalho, SFG and Moraes-Souza, H (2018) Socio-epidemiological characterization of blood donors with asymptomatic Leishmania infantum infection from three Brazilian endemic regions and analysis of the transfusional transmission risk of visceral leishmaniasis. Transfusion Medicine 28, 433–439.CrossRefGoogle Scholar
Foulet, F, Botterel, F, Buffet, P, Morizot, G, Rivollet, D, Deniau, M, Pratlong, F, Costa, J-M and Bretagne, S (2007) Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome b gene. Journal of Clinical Microbiology 45, 2110–2115.CrossRefGoogle ScholarPubMed
França, OA, De Castro, VL, Pontes, ER and Dorval, ME (2013) Anti Leishmania antibodies in blood donors from the Midwest region of Brazil. Transfusion Apheresis 46, 627–630.CrossRefGoogle Scholar
Galluzzi, L, Ceccarelli, M, Diotallevi, A, Menotta, M and Magnani, M (2018) Real-time PCR applications for diagnosis of leishmaniasis. Parasites & Vectors 11, 1–13.CrossRefGoogle ScholarPubMed
Gomes, CM, Cesetti, MV, Paula, NA, Vernal, S, Gupta, G, Sampaio, RNR and Roselino, AM (2007) Field validation of SYBR Green – and TaqMan-based real-time PCR using biopsy and swab samples to diagnose American tegumentary leishmaniasis in an area where Leishmania (Viannia) braziliensis is endemic. Journal of Clinical Microbiology 55, 526–534.CrossRefGoogle Scholar
Gómez-Hernárdez, C, Bento, EC, Rezende-Oliveira, K, Nascentes, GAN, Barbosa, CG, Batista, LR, Tiburcio, M, Pedrosa, AL, Lages-Silva, E, Ramirez, JD and Ramirez, LE (2017) Leishmania infection in bats from a non-endemic region of leishmaniasis in Brazil. Parasitology 144, 1–7.Google Scholar
Grogl, M, Daugirda, JL, Hoover, DL, Magill, AJ and Berman, JD (1993) Survivability and infectivity of viscerotropic Leishmania tropica from Operation Desert Storm participants in human blood products maintained under blood bank conditions. The America Journal of Tropical Medicine and Hygiene 49, 308–315.CrossRefGoogle ScholarPubMed
Jimenez-Marco, T, Riera, C, Fisa, R, Girona-Llobera, E, Sedeño, M, Goodrich, RP, Pujol, A, Guillen, C and Muncunill, J (2012) The utility of pathogen inactivation technology: a real life example of Leishmania infantum inactivation in platelets from a donor with asymptomatic infection. Blood Transfusion 10, 536–541.Google ScholarPubMed
Kaushal, H, Bhattacharya, SK, Verma, S and Salotra, P (2017) Serological and molecular analysis of Leishmania infection in healthy individuals from two districts of West Bengal, India, endemic for visceral leishmaniasis. The American Journal of Tropical Medicine and Hygiene 96, 1448–1455.CrossRefGoogle ScholarPubMed
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.CrossRefGoogle ScholarPubMed
Luyo-Acero, GE, Uezato, H, Oshiro, M, Takei, K, Karika, K, Katakura, K, Gomez-Landires, E, Hashiguchi, Y and Nokaka, S (2009) Sequence variation of the cytochrome b gene of various human infecting members of the genus Leishmania and their phylogeny. Cambridge Journal 128, 483–491.Google Scholar
Luz, KG, Silva, VO, Gomes, EM, Machado, FCS, Araújo, MAF, Fonseca, HEM, Freire, TC, D'almeida, JB, Palatinik, M and Palatinik-De-Sousa, CB (1997) Prevalence of anti-Leishmania donovani antibody among Brazilian blood donors and multiply transfused hemodialysis patients. The American Journal of Tropical Medicine and Hygiene 57, 168–171.CrossRefGoogle ScholarPubMed
Mauricio, IL, Gaunt, MW, Stothard, JR and Miles, MA (2001) Genetic typing and phylogeny of the Leishmania donovani complex by restriction analysis of PCR amplified gp63 intergenic regions. Parasitology 122, 393–403.CrossRefGoogle ScholarPubMed
Medeiros, FAC, Gomes, LI, Oliveira, E, Souza, CSA, Mourão, MV, Cota, GF, Marques, LHS, Carneiro, M and Rabello, A (2017) Development and validation of a PCR-ELISA for the diagnosis of symptomatic and asymptomatic infection by Leishmania (Leishmania) infantum. Journal of Tropical Medicine, 1–10. doi: 10.1155/2017/7364854.CrossRefGoogle Scholar
Menezes, JA, Luz, TCB, Sousa, FFS, Verne, RN, Lima, FP and Margonari, C (2016) Peridomiciliary risk factors and knowledge concerning visceral leishmaniasis in the population of Formiga, Minas Gerais, Brazil. Revista Brasileira de Epidemiologia 19, 362–374.CrossRefGoogle ScholarPubMed
Michel, G, Pomares, C, Ferrua, B and Marty, P (2011) Importance of worldwide asymptomatic carriers of Leishmania infantum (L. chagasi) in human. Acta Tropica 119, 69–75.CrossRefGoogle ScholarPubMed
Miot, HA (2016) Análise de concordância em estudos clínicos e experimentais. Jornal Vascular Brasileiro 15, 89–92.CrossRefGoogle Scholar
Mpaka, MA, Daniil, Z, Kyriakou, DS and Zakynthinos, E (2009) Septic shock due to visceral leishmaniasis, probably transmitted from blood transfusion. Journal of Infection in Developing Countries 3, 479–483.CrossRefGoogle ScholarPubMed
Nicolas, L, Prina, E, Lang, T and Milon, G (2002) Real-time PCR for detection and quantitation of Leishmania in mouse tissues. Journal of Clinical Microbiology 40, 1666–1669.CrossRefGoogle ScholarPubMed
Nicolas, L, Prina, E, Lang, T and Milon, G ( (2002) Real-time PCR for detection and quantitation of leishmania in mouse tissues. Journal of Clinical Microbiology 40, 1666–1669.CrossRefGoogle ScholarPubMed
Pereira, MR, Rocha-Silva, F, Graciele-Melo, C, Lafuente, CR, Magalhães, T and Caligiorne, RB (2014) Comparison between conventional and real-time PCR assays for diagnosis of visceral leishmaniasis. BioMed Research International 6, 1–4.Google Scholar
Pessoa-E-Silva, R, Vaitkevicius-Antão, V, Andrade, TAS, Silva, ACO, Oliveira, GA, Trajano-Silva, LAM, Nakasone, EKN and Paiva-Cavalcanti, M (2019) The diagnosis of canine visceral leishmaniasis in Brazil: confronting old problems. Experimental Parasitology 199, 9–16.CrossRefGoogle Scholar
Ramírez, JD, Hernández, C, Léon, CM, Ayala, MS, Flórez, C and González, C (2016) Taxonomy, diversity, temporal and geographical distribution of cutaneous leishmaniasis in Colombia: a retrospective study. Scientific Reports 6, 1–10.CrossRefGoogle ScholarPubMed
Reithinger, R and Dujardin, J-C (2007) Molecular diagnosis of leishmaniasis: current status and future applications. Journal of Clinical Microbiology 45, 21–25.CrossRefGoogle ScholarPubMed
Rodríguez-Cortés, A, Ojeda, A, López-Fuertes, L, Timón, M, Altet, L, Solano-Gallego, L, Sánchez-Robert, E, Francino, O and Alberola, J (2007) A long term experimental study of canine visceral leishmaniasis. International Journal for Parasitology 37, 683–693.CrossRefGoogle ScholarPubMed
Romero, HD, Silva, LAA, Silva-Vergara, ML, Rodrigues, V, Costa, RT, Guimarães, SF, Alecrim, W, Moraes-Souza, H and Prata, A (2009) Comparative study of serologic tests for the diagnosis of asymptomatic visceral leishmaniasis in an endemic area. The American Journal of Tropical Medicine and Hygiene 81, 27–33.CrossRefGoogle Scholar
Ruiter, CM, Veer, CVD, Leeflang, MMG, Deborggraeve, S, Lucas, C and Adams, ER (2014) Molecular tools for diagnosis of visceral leishmaniasis: systematic review and meta-analysis of diagnostic test accuracy. Journal of Clinical Microbiology 52, 3147–3155.CrossRefGoogle ScholarPubMed
Silva, LA, Romero, HD, Nogueira Nascentes, GA, Costa, RT, Rodrigues, V and Prata, A (2011) Antileishmania immunological tests for asymptomatic subjects living in a visceral leishmaniasis-endemic area in Brazil (2011). The American Journal of Tropical Medicine and Hygiene 84, 261–266.CrossRefGoogle Scholar
Silva, LA, Homero, HD, Fagundes, A, Nehme, N, Fernandes, O, Rodrigues, V, Costa, RT and Prata, A (2013) Use of the polymerase chain reaction for the diagnosis of asymptomatic Leishmania infection in a visceral leishmaniasis-endemic area. Revista do Instituto de Medicina Tropical de São Paulo 55, 1–4.CrossRefGoogle Scholar
Sudarshan, M and Sundar, S (2014) Parasite load estimation by qPCR differentiates between asymptomatic and symptomatic infection in Indian visceral leishmaniasis. Diagnostic Microbiology and Infectious Disease 80, 40–42.CrossRefGoogle ScholarPubMed
Sudarshan, M, Singh, T, Singh, A and Singh, A (2014) Quantitative PCR in Epidemiology for Early Detection of Visceral Leishmaniasis Cases in India. PLOS Neglected Tropical Diseases, 8, 1–6. doi: 10.1371/journal.pntd.0003366.CrossRefGoogle ScholarPubMed
Sudarshan, M, Singh, T, Singh, AK, Chourasia, A, Singh, B, Wilson, ME, Chakravarty, J and Sundar, S (2014) Quantitative PCR in epidemiology for early detection of visceral leishmaniasis cases in India. PLOS Neglected Tropical Diseases 8, 1–6.CrossRefGoogle ScholarPubMed
Uliana, SRB, Affonso, MHT, Camargo, EP and Floeter-Winter, LM (1991) Leishmania: genus identification based on a specific sequence of the 18S ribosomal RNA sequence. Experimental Parasitology 72, 157–163.CrossRefGoogle ScholarPubMed
Urias, EVR, Carvalho, SFG, Oliveira, CL, Carvalho, MLM, Teles, LF, Rodrigues, MC and Maia, CN (2009) Prevalência de adultos infectados por Leishmania Leishmania chagasi entre doadores de sangue do Hemocentro Regional de Montes Claros, Minas Gerais, Brasil. Revista Brasileira de Hematologia e Hemoterapia 31, 348–354.CrossRefGoogle Scholar
Varani, S, Ortalli, M, Attard, L, Vanino, E, Gaibani, P, Vocale, C, Rossini, G, Cagarelli, R, Pierros, A, Billi, P, Mastroianni, A, Di, Cesare, S, Codeluppi, M, Franceschini, E, Melchiondas, F, Gramiccias, M, Scalone, A, Gentilomi, GA and Landin, MP (2017) Serological and molecular tools to diagnose visceral leishmaniasis: 2-years’ experience of a single center in Northern Italy. PLoS ONE 12, 1–10.CrossRefGoogle ScholarPubMed
WHO. World Health Organization (2020) Leishmaniasis: visceral leishmaniasis. Available at https://www.who.int/news-room/fact-sheets/detail/leishmaniasis (Accessed 27 August 2020).Google Scholar