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Immunocytochemical demonstration of a SALMFamide-like neuropeptide in the nervous system of adult and larval stages of the human blood fluke, Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

D. J. A. Brownlee ,
I. Fairweather ,
C. F. Johnston ,
M. C. Thorndyke  and
P. J. Skuce
D. J. A. Brownlee
Affiliation:
Department of Physiology and Pharmacology, School of Biomedical Sciences, University of Southampton, Southampton SO16 7PX, England School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 1NN, NorthernIreland
I. Fairweather
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 1NN, NorthernIreland
C. F. Johnston
Affiliation:
School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 1NN, NorthernIreland
M. C. Thorndyke
Affiliation:
Royal Holloway and Bedford New College, University of London, Egham, Surrey TW20 0EX, England
P. J. Skuce
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 1NN, NorthernIreland
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Summary

The localization and distribution of SALMFamide immunoreactivity (IR), SI(GFNSALMFamide), in the nervous system of both the adult and larval stages of the trematode Schistosoma mansoni has been determined by an indirect immunofluorescent technique in conjunction with confocal scanning laser microscopy (CSLM). Immunostaining was widespread in the nervous system of adult male and female S. mansoni. In the central nervous system (CNS), IR was evident in nerve cells and fibres in the anterior ganglia, cerebral commissure and dorsal and ventral nerve cords. In the peripheral nervous system (PNS), IR was apparent in nerve plexuses associated with the subtegmental musculature, oral and ventral suckers, the lining of the gynaecophoric canal, and in fine nerve fibres innervating the dorsal tubercles of the male worm. In the reproductive system of male and female worms, Sl-IR was only observed around the ootype/Mehlis' gland complex in the female. Immunostaining was also evident in the nervous system of both miracidium and cercarial larval stages. A post-embedding, IgG-conjugated colloidal gold immunostaining technique was employed to examine the subcellular distribution of SALMFamide-IR in the CNS of S. mansoni. Gold labelling of peptide was localized over dense-cored vesicles within nerve cell bodies and fibres constituting the neuropile of the anterior ganglia, cerebral commissure and nerve cords of the CNS. Antigen pre-absorption studies indicated that the results obtained do suggest S1-like immunostaining and not cross-reactivity with other peptides, in particular FMRFamide.

Keywords

Schistosoma mansoniplatyhelminthTrematodamiracidiumcercariaadultneuropeptide SALMFamideFMRFamideconfocal scanning laser microscopy (CSLM)immunogold labelling
Type
Research Article
Information
Parasitology , Volume 110 , Issue 2 , February 1995 , pp. 143 - 153
Copyright
Copyright © Cambridge University Press 1995

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