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Intranasal immunization with LACK-DNA promotes protective immunity in hamsters challenged with Leishmania chagasi

Published online by Cambridge University Press:  26 August 2011

DANIEL CLAUDIO DE OLIVEIRA GOMES ,
BEATRIZ LILIAN DA SILVA COSTA SOUZA ,
HERBERT LEONEL DE MATOS GUEDES ,
ULISSES GAZOS LOPES  and
BARTIRA ROSSI-BERGMANN
DANIEL CLAUDIO DE OLIVEIRA GOMES*
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, RJ, Brazil Núcleo de Doenças Infecciosas, Universidade Federal do Espírito Santo, 29040-091, Vitória, ES, Brazil
BEATRIZ LILIAN DA SILVA COSTA SOUZA
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, RJ, Brazil
HERBERT LEONEL DE MATOS GUEDES
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, RJ, Brazil
ULISSES GAZOS LOPES
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, RJ, Brazil
BARTIRA ROSSI-BERGMANN
Affiliation:
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, RJ, Brazil
*
*Corresponding author: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902, Rio de Janeiro, RJ, Brazil. Tel/Fax: 55 (27) 33357210 and 55 (27) 33357207. E-mail: dgomes@ndi.ufes.br
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Summary

LACK (Leishmania analogue of the receptor kinase C) is a conserved protein in protozoans of the genus Leishmania which is associated with the immunopathogenesis and susceptibility of BALB/c mice to L. major infection. Previously, we demonstrated that intranasal immunization with a plasmid carrying the LACK gene of Leishmania infantum (LACK-DNA) promotes protective immunity in BALB/c mice against Leishmania amazonensis and Leishmania chagasi. In the present study, we investigated the protective immunity achieved in hamsters intranasally vaccinated with 2 doses of LACK-DNA (30 μg). Compared with controls (PBS and pCI-neo plasmid), animals vaccinated with LACK-DNA showed significant reduction in parasite loads in the spleen and liver, increased lymphoproliferative response and increased nitric oxide (NO) production by parasite antigen-stimulated splenocytes. Furthermore, hamsters vaccinated with LACK-DNA presented high IgG and IgG2a serum levels when compared to control animals. Our results showed that intranasal vaccination with LACK-DNA promotes protective immune responses in hamsters and demonstrated the broad spectrum of intranasal LACK-DNA efficacy in different host species, confirming previous results in murine cutaneous and visceral leishmaniasis.

Keywords

Leishmania chagasihamsterLACK-DNAmucosal vaccine
Type
Research Article
Information
Parasitology , Volume 138 , Issue 14 , December 2011 , pp. 1892 - 1897
Copyright
Copyright © Cambridge University Press 2011

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