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Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei

Published online by Cambridge University Press:  05 July 2010

GREGORY S. RICHMOND ,
FEDERICA GIBELLINI ,
SIMON A. YOUNG ,
LOUISE MAJOR ,
HELEN DENTON ,
ALISON LILLEY  and
TERRY K. SMITH
FEDERICA GIBELLINI
Affiliation:
Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
SIMON A. YOUNG
Affiliation:
Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
LOUISE MAJOR
Affiliation:
Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
HELEN DENTON
Affiliation:
Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
ALISON LILLEY
Affiliation:
Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
TERRY K. SMITH*
Affiliation:
Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
*
*Corresponding author: Tel: (0)1334-463412; E-mail: tks1@st-andrews.ac.uk
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Summary

The biological membranes of Trypanosoma brucei contain a complex array of phospholipids that are synthesized de novo from precursors obtained either directly from the host, or as catabolised endocytosed lipids. This paper describes the use of nanoflow electrospray tandem mass spectrometry and high resolution mass spectrometry in both positive and negative ion modes, allowing the identification of ~500 individual molecular phospholipids species from total lipid extracts of cultured bloodstream and procyclic form T. brucei. Various molecular species of all of the major subclasses of glycerophospholipids were identified including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol as well as phosphatidic acid, phosphatidylglycerol and cardolipin, and the sphingolipids sphingomyelin, inositol phosphoceramide and ethanolamine phosphoceramide. The lipidomic data obtained in this study will aid future biochemical phenotyping of either genetically or chemically manipulated commonly used bloodstream and procyclic strains of Trypanosoma brucei. Hopefully this will allow a greater understanding of the bizarre world of lipids in this important human pathogen.

Keywords

PhospholipidTrypanosoma bruceimass spectrometrylipidomics
Type
Research Article
Information
Parasitology , Volume 137 , Special Issue 9: Insights into the metabolomes of parasites , August 2010 , pp. 1357 - 1392
Copyright
Copyright © Cambridge University Press 2010

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