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Mitochondrial DNA sequence variation among geographical isolates of Opisthorchis viverrini in Thailand and Lao PDR, and phylogenetic relationships with other trematodes

Published online by Cambridge University Press:  21 October 2008

W. SAIJUNTHA ,
P. SITHITHAWORN ,
S. WONGKHAM ,
T. LAHA ,
N. B. CHILTON ,
T. N. PETNEY ,
M. BARTON  and
R. H. ANDREWS
W. SAIJUNTHA
Affiliation:
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
P. SITHITHAWORN*
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
S. WONGKHAM
Affiliation:
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
T. LAHA
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
N. B. CHILTON
Affiliation:
Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
T. N. PETNEY
Affiliation:
Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany
M. BARTON
Affiliation:
School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia
R. H. ANDREWS
Affiliation:
School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia
*
*Corresponding author: Department of Parasitology and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Tel: +66 43348387. Fax: +66 43202475. E-mail: paib_sit@kku.ac.th
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Summary

The present study compared the genetic variation among 14 different geographical isolates of Opisthorchis viverrini sensu lato from Thailand and Lao PDR using sequence data for 2 mitochondrial DNA genes, the subunit 1 of NADH dehydrogenase gene (nad1) and cytochrome c oxidase gene (cox1). Four different nad1 haplotypes were detected among isolates, all of which were identical at the amino acid sequence level. Nucleotide sequence variation among 14 isolates ranged from 0 to 0·3% for nad1. Two different cox1 haplotypes were detected among isolates. These two haplotypes differed at 2 nucleotide positions, one of which resulted in a change in the amino acid sequence. Nucleotide sequence variation among isolates for cox1 ranged from 0 to 0·5%. Comparison of cox1 sequences of O. viverrini to those of other trematodes revealed nucleotide differences of 13–31%. A phylogenetic analysis of the cox1 sequence data revealed strong statistical support for a clade containing O. viverrini and 2 other species of opisthorchid trematodes; O. felineus and Clonorchis sinsensis.

Keywords

Opisthorchis viverrinitrematodesgenetic variationmitochondrial DNANADH dehydrogenase subunit 1 genecytochrome c oxidase subunit 1 genephylogenetic relationships
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 12 , October 2008 , pp. 1479 - 1486
Copyright
Copyright © 2008 Cambridge University Press

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