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Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica

Published online by Cambridge University Press:  29 August 2006

K. CHAITHIRAYANON ,
R. GRAMS ,
S. VICHASRI-GRAMS ,
A. HOFMANN ,
G. KORGE ,
V. VIYANANT ,
E. S. UPATHAM  and
P. SOBHON
K. CHAITHIRAYANON
Affiliation:
Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand
R. GRAMS
Affiliation:
Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Phahonyothin Rd., Klongluang, Pathumthani, 12120, Thailand
S. VICHASRI-GRAMS
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand
A. HOFMANN
Affiliation:
Institut für Biologie-Genetik, Freie Universität Berlin, Arnimallee 7, Berlin 14195, Germany
G. KORGE
Affiliation:
Institut für Biologie-Genetik, Freie Universität Berlin, Arnimallee 7, Berlin 14195, Germany
V. VIYANANT
Affiliation:
Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Phahonyothin Rd., Klongluang, Pathumthani, 12120, Thailand
E. S. UPATHAM
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand
P. SOBHON
Affiliation:
Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand
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Abstract

A cDNA encoding Fg14-3-3 protein 1 was cloned by immunoscreening of an adult-stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental antigens of the parasite. The protein has a deduced amino acid sequence of 252 residues and a calculated molecular weight of 28·7 kDa. It shows sequence identity values between 57·6 and 58·1% to the human 14-3-3 beta, zeta, theta, and eta proteins and is in a phylogenetic cluster with the 14-3-3 protein 1 of Schistosoma spp. Nucleic acid analyses indicate that the Fg14-3-3 protein 1 is encoded by a single copy gene and that this gene is expressed as a transcript of 1250 nucleotides. In adult and 4-week-old parasites the gene's transcriptional and translational products were localized in the gut epithelium, parenchyma, tegument cells, and in the reproductive organs. An antiserum against recombinant Fg14-3-3 protein 1 detected a slightly smaller 14-3-3 protein in the parasite's excretion/secretion material and showed cross-reactivity with 14-3-3 proteins in extracts of other trematodes and mouse. Antibodies against Fg14-3-3 protein were detected in the sera of rabbits as early as 2 weeks after infection with metacercariae of F. gigantica and the antibody titre increased continuously over a 10-week observation period.

Keywords

Fasciola gigantica14-3-3 proteintegumental antigensexpressionlocalization
Type
Research Article
Information
Parasitology , Volume 133 , Issue 6 , December 2006 , pp. 763 - 775
Copyright
2006 Cambridge University Press

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