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Schistosoma mansoni: ability of Con A to protect in vitro mechanically transformed schistosomula against the lethal effect of immune serum plus complement

Published online by Cambridge University Press:  06 April 2009

Thea A. van Pijkeren ,
C. A. P. Tavares  and
G. Gazzinelli
Thea A. van Pijkeren
Affiliation:
Departamento de Bioquimica-Imunologia, Institute de Ciências Biológicas, UFMG
C. A. P. Tavares
Affiliation:
Departamento de Bioquimica-Imunologia, Institute de Ciências Biológicas, UFMG
G. Gazzinelli
Affiliation:
Centro de Pesquisas René Rachou, C.P. 1743, Belo Horizonte, Brasil
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Summary

Concanavalin A added to a defined culture medium renders schistosomula more resistant to the lethal effect of antibody plus complement. The Con A dose-dependent acquisition of protection reached a plateau at a concentration of 4 μg Con A/ml. α-Methylmannoside was not able to reverse the Con A-induced protection but excess methylmannoside, added to the incubation medium together with Con A partially prevented the effect of the lectin. Binding of anti-parasite antibody to the schistosomula surface did not change after pre-incubation with Con A. In the presence of puromycin (100 μg/ml) Con A is still able to confer protection. Incorporation of labelled amino acid into the tegumental proteins was not affected by Con A. Furthermore, a progressive reduction of protection was observed as the time of culture preceding Con A addition increased.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 2 , April 1982 , pp. 239 - 252
Copyright
Copyright © Cambridge University Press 1982

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