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Some factors affecting the cultivation in vitro of the erythrocytic stages of Plasmodium knowlesi

Published online by Cambridge University Press:  06 April 2009

P. I. Trigg
P. I. Trigg
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London, N. W. 7
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Extract

A study was made of some of the factors which affect the growth of Plasmodium knowlesi in blood diluted in the Harvard medium. Some slight enhancement of growth and re-invasion of new host cells was obtained when P. knowlesi was incubated in low oxygen tensions. Some growth but no re-invasion was obtained under anaerobic conditions. High oxygen tensions were also inhibitory.

The lipids present in the plasma are important for the growth of P. knowlesi in vitro. Stearic acid and cholesterol are required by the parasite but do not account for all the growth promoting properties of plasma. A requirement for vitamin B12 was also demonstrated.

I should like to thank Dr F. Hawking and Dr J. Williamson for their advice and for reading through the manuscript, Mr T. J. Scott-Finnigan for technical assistance, Dr K. Mervyn (Glaxo Research Ltd.) for samples of vitamin B12 analogues and the Hachmeister Company (Pittsburgh) for a sample of TEM 4T. This work received financial assistance from the World Health Organization.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 4 , November 1969 , pp. 915 - 924
Copyright
Copyright © Cambridge University Press 1969

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References

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