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Ultrastructural localization of pancreatic polypeptide- and FMRFamide immunoreactivities within the central nervous system of the nematode, Ascaris suum (Nematoda: Ascaroidea)

Published online by Cambridge University Press:  06 April 2009

D. J. A. Brownlee ,
G. P. Brennan ,
D. W. Halton ,
I. Fairweather  and
C. Shaw
D. J. A. Brownlee
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
G. P. Brennan
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
D. W. Halton
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
I. Fairweather
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
C. Shaw
Affiliation:
School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
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Summary

A post-embedding immunogold technique has been used to examine the subcellular distribution of immunoreactivities to vertebrate pancreatic polypeptide (PP) and to the invertebrate peptide, FMRFamide within the central nervous system (CNS) of the nematode, Ascaris suum. Gold labelling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the main ganglia and nerve cords in the CNS. Double-labelling of peptides demonstrated an apparent co-localization of PP and FMRFamide immunoreactivities in the same dense-cored vesicles, although populations of dense-cored vesicles that labelled solely for FMRFamide were also evident. Antigen preabsorption studies indicated little or no cross-reactivity between the two antisera.

Keywords

Ascaris suumNematodaneuropeptidespancreatic polypeptideFMRFamideimmunogold labellingultrastructurenervous system
Type
Research Article
Information
Parasitology , Volume 108 , Issue 5 , June 1994 , pp. 587 - 593
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Atkinson, H. J., Isaac, R. E., Harris, P. D. & Sharpe, C. M. (1988). FMRFamide-like immunoreactivity within the nervous system of the nematodes Panagrellus redivivus, Caenorhabditis elegans and Heterodera glycines. Journal of Zoology 216, 663–71.CrossRefGoogle Scholar
Brennan, G. P., Halton, D. W., Maule, A. G., Shaw, C., Johnston, C. F., Moore, S. & Fairweather, I. (1993 a). Immunoelectron microscopical studies of regulatory peptides in the nervous system of the monogenean parasite, Diclidophora merlangi. Parasitology 106, 171–6.CrossRefGoogle Scholar
Brennan, G. P., Halton, D. W., Maule, A. G. & Shaw, C. (1993 b). Electron immunogold-labelling of regulatory peptide immunoreactivity in the nervous system of Moniezia expansa (Cestoda: Cyclophyllidea). Parasitology Research 79, 409–15.CrossRefGoogle Scholar
Brownlee, D. J. A., Fairweather, I., Johnston, C. F., Smart, D., Shaw, C. & Halton, D. W. (1993 a). Immunocytochemical demonstration of neuropeptides in the central nervous system of the roundworm, Ascaris suum (Nematoda, Ascaroidea). Parasitology 106, 305–16.CrossRefGoogle ScholarPubMed
Brownlee, D. J. A., Fairweather, I. & Johnston, C. F. (1993 b). Immunocytochemical demonstration of neuropeptides in the peripheral nervous system of the roundworm, Ascaris suum (Nematoda, Ascaroidea). Parasitology Research 79, 302–8.CrossRefGoogle ScholarPubMed
Brownlee, D. J. A., Fairweather, I., Johnston, C. F. & Shaw, C. (1993 c). Immunocytochemical demonstration of peptidergic and serotoninergic components in the enteric nervous system of the roundworm, Ascaris suum (Nematoda, Ascaroidea). Parasitology 108, 89–103.CrossRefGoogle Scholar
Callaway, J. C., Masinovsky, B. & Craubard, K. (1987). Co-localization of SCPB-like and FMRFamide-like immunoreactivities in crustacean nervous systems. Brain Research 405, 295–304.CrossRefGoogle ScholarPubMed
Cowden, C., Sithigorngul, P., Brackley, P., Guastella, J. & Stretton, A. O. W. (1993). Localization and differential expression of FMRFamide-like immunoreactivity in the nematode Ascaris suum. Journal of Comparative Neurology 333, 455–68.CrossRefGoogle ScholarPubMed
Cowden, C., Sithigorncul, P., Guastella, J. & Stretton, A. O. W. (1987). FMRF-amide-like peptides in Ascaris suum. American Zoologist 27, 127A.Google Scholar
Cowden, C. & Stretton, A. O. W. (1990). AF2, a nematode neuropeptide. Neuroscience 16, 305.Google Scholar
Cowden, C. & Stretton, A. O. W. (1993). AF2, an Ascaris neuropeptide: isolation, sequence, and bioactivity. Peptides 14, 423–30.CrossRefGoogle ScholarPubMed
Cowden, C., Stretton, A. O. W. & Davis, R. E. (1989). AF1, a sequenced bioactive neuropeptide isolated from the nematode Ascaris suum. Neuron 2, 1465–73.CrossRefGoogle ScholarPubMed
Davenport, T. R. B., Lee, D. L. & Issac, R. E. (1988). Immunocytochemical demonstration of a neuropeptide in Ascaris suum (Nematoda) using an antiserum to FMRFamide. Parasitology 97, 81–8.CrossRefGoogle ScholarPubMed
Evans, B. D. & Calabrese, R. L. (1989). Small cardioactive peptide-like immunoreactivity and its colocalization with FMRFamide-like immunoreactivity in the central nervous system of the leech Hirudo medicinalis. Cell and Tissue Research 257, 187–99.CrossRefGoogle ScholarPubMed
Geary, T. G., Price, D. A., Bowman, J. W., Winterrowd, C. A., Mackenzie, C. D., Garrison, R. D., Williams, J. F. & Friedman, A. R. (1992). Two FMRFamide-like peptides from the free-living nematode Panagrellus redivivus. Peptides 13, 209–14.CrossRefGoogle ScholarPubMed
Grimmelikhuijzen, C. J. P. (1983). Coexistence of neuropeptides in Hydra. Neuroscience 9, 837–45.CrossRefGoogle ScholarPubMed
Gustafsson, M. K. S. & Wikgren, M. C. (1989). Development of immunoreactivity to the invertebrate neuropeptide small cardiac peptide B in the tapeworm Diphyllobothrium dendriticum. Parasitology Research 75, 396–400.CrossRefGoogle Scholar
Hökfelt, I., Holets, V. R., Staines, W., Meister, B., Melander, T., Schalling, M., Schultzberg, M., Freedman, J., Björklund, H., Olson, L., Lindh, B., Elevin, L.-G., Lundberg, J. M., Lindgren, J. Å., Samuelsson, B., Pernow, B., Terenius, L., Post, C., Everitt, B. & Goldstein, M. (1986). Coexistence of neuronal messengers – an overview. Progress in Brain Research 68, 33–70.CrossRefGoogle ScholarPubMed
Jung, L. J. & Scheller, R. H. (1991). Peptide processing and targeting in the neuronal secretory pathway. Science 25, 1330–5.CrossRefGoogle Scholar
Maule, A. G., Brennan, G. P., Halton, D. W., Shaw, C., Johnston, C. F. & Moore, S. (1992). Neuropeptide Fimmunoreactivity in the monogenean parasite Diclidophora merlangi. Parasitology Research 78, 655–60.CrossRefGoogle ScholarPubMed
Reuter, M., Gustafsson, M. K. S., Lang, J. & Grimmelikhuijzen, C. J. P. (1990). The release sites and targets of nerve cells immunoreactive to RFamide – an ultrastructural study of Microstomum lineare and Diphyllobothrium dendriticum (Plathelminthes). Zoomorphology 109, 303–8.CrossRefGoogle Scholar
Rosoff, M. L., Doble, K. E., Price, D. A. & Li, C. (1993). The flp-1 propeptide is processed into multiple, highly similar FMRFamide-like peptides in Caenorhabditis. Peptides 14, 331–8.CrossRefGoogle ScholarPubMed
Roth, J. (1982). The protein A-gold (pAg) technique. Qualitative and quantitative approach for antigen localisation on thin sections. In Techniques in Immunocytochemistry, Vol. 1, (ed. Bullock, G. R. & Petrusz, P.), pp. 104–37. London: Academic Press.Google ScholarPubMed
Schinkmann, K. & Li, C. (1992). Localization of FMRFamide-like peptides in Caenorhabditis elegans. Journal of Comparative Neurology 316, 251–60.CrossRefGoogle ScholarPubMed
Sithigorngul, P., Stretton, A. O. W. & Cowden, C. (1990). Neuropeptide diversity in Ascaris: an immunocytochemical study. Journal of Comparative Neurology 294, 362–76.CrossRefGoogle ScholarPubMed
Stretton, A. O. W., Cowden, C., Sithigorngul, P. & Davis, R. E. (1991). Neuropeptides in the nematode Ascaris suum. Parasitology 102, S107–16.CrossRefGoogle ScholarPubMed
Stretton, A., Donmoyer, J., Davis, R., Meade, J., Cowden, C. & Sithigorngul, P. (1992). Motor behavior and motor nervous system function in the nematode Ascaris suum. Journal of Parasitology 78, 206–14.CrossRefGoogle ScholarPubMed
Takayanagi, H. & Takeda, N. (1988). Coexistence of FMRFamide, met-enkephalin and serotonin in molluscan neurons. Comparative Biochemistry and Physiology 91A, 613–20.Google ScholarPubMed