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Use of fluorescent probes as a useful tool to identify resistant Schistosoma mansoni isolates to praziquantel

Published online by Cambridge University Press:  21 June 2010

F. F. B. COUTO ,
P. M. Z. COELHO ,
N. ARAÚJO ,
J. R. KUSEL ,
N. KATZ  and
A. C. A. MATTOS
F. F. B. COUTO
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
P. M. Z. COELHO*
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
N. ARAÚJO
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
J. R. KUSEL
Affiliation:
Division of Infection and Immunity, IBLS, University of Glasgow, Scotland, UK
N. KATZ
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
A. C. A. MATTOS*
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
*
*Corresponding authors: Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil. Tel: +55 31 3349 7740/ +55 31 3349 7759. Fax: +5531 3295 3115. E-mail: coelhopm@cpqrr.fiocruz.br/anademattos@cpqrr.fiocruz.br
*Corresponding authors: Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil. Tel: +55 31 3349 7740/ +55 31 3349 7759. Fax: +5531 3295 3115. E-mail: coelhopm@cpqrr.fiocruz.br/anademattos@cpqrr.fiocruz.br
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Summary

The use of chemotherapy on a mass scale in endemic areas may lead to the appearance of resistant isolates through the mechanism of selective drug pressure. Studies have demonstrated that praziquantel (PZQ) is able to inhibit the excretory activity and to cause tegumental damage in Schistosoma mansoni adult worms. The use of the probe resorufin to evaluate excretory activity, as well as the probe Hoechst 33258 to detect tegumental damage in adult worms, may represent a method to identify resistant (or less susceptible) isolates. The purpose of the present work was to compare the changes caused by PZQ in the function of the excretory system and in the integrity of the tegument of adult worms from the LE isolate (susceptible to PZQ) and the LE-PZQ isolate (less susceptible to PZQ). Worms from the isolate LE-PZQ showed less severe tegumental lesions, in both in vitro and in vivo experiments, detected by labelling with Hoechst 33258 and continued to have a functional excretory system as shown by labelling with resorufin in vitro.

Keywords

Schistosoma mansonidrug resistancepraziquantelfluorescent probes
Type
Research Article
Information
Parasitology , Volume 137 , Issue 12 , October 2010 , pp. 1791 - 1797
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

Becker, B., Mehlhorn, H., Andrews, P., Thomas, H. and Eckert, J. (1980). Light and electron microscopic studies on the effect of praziquantel on Schistosoma mansoni, Dicrocoelium dendriticum, and Fasciola hepatica (Trematoda) in vitro. Zeitschrift für Parasitenkunde 63, 113–128.CrossRefGoogle ScholarPubMed
Bosch, I. B., Wang, Z. X., Tao, L. C. S. and Schoemaker, C. B. (1994). Two Schistosoma mansoni cDNA encoding ATP-binding cassette (ABC) family proteins. Molecular and Biochemical Parasitology 65, 351–356.Google Scholar
Cioli, D. and Pica-Mattoccia, L. (2003). Praziquantel. Parasitology Research 90, (Suppl.) S3–S9.Google Scholar
Coelho, P. M. Z., Lima, F. C. S. and Nogueira, J. A. M. (1997). Resistance to oxamniquine of a Schistosoma mansoni strain isolate from a patient submitted to repeated treatments. Revista do Instituto de Medicina Tropical de São Paulo 39, 101–106.CrossRefGoogle ScholarPubMed
Coles, G. C. and Kinoti, G. K. (1997). Defining resistance in Schistosoma. Parasitology Today 13, 157–158.Google Scholar
Doenhoff, M. J., Kusel, J. R., Coles, G. C. and Cioli, D. (2002). Resistance of Schistosoma mansoni to praziquantel: is there a problem? Transactions of the Royal Society of Tropical Medicine and Hygiene 96, 465–469.CrossRefGoogle ScholarPubMed
Doenhoff, M. J., Sabah, A. A., Fletcher, C., Webbe, G. and Bain, J. (1987). Evidence for an immune-dependent action of praziquantel on Schistosoma mansoni in mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 947–951.CrossRefGoogle ScholarPubMed
Fetterer, R. H., Pax, R. A. and Bennett, J. L. (1980). Praziquantel, potassium and 2,4-dinitrophenol: analysis of their action on the musculature of Schistosoma mansoni. European Journal of Pharmacology 64, 31–38.CrossRefGoogle ScholarPubMed
Harnett, W. and Kusel, J. R. (1986). Increased exposure of parasite antigens at the surface of adult male Schistosoma mansoni. Parasite Imunology 7, 417–428.Google Scholar
Ismail, M., Metwally, A., Farghaly, A., Bruce, J., Tao, L. F. and Bennett, J. L. (1996). Characterization of isolates of Schistosoma mansoni from Egyptian villagers that tolerate high doses of praziquantel. The American Journal of Tropical Medicine and Hygiene 55, 214–218.CrossRefGoogle ScholarPubMed
Kusel, J. K., Al-Adhami, B. H. and Doenhoff, M. J. (2007). The schistosome in the mammalian host: understanding the mechanism of adaptation. Parasitology 134, 1477–1526.CrossRefGoogle ScholarPubMed
Kusel, J. R., Oliveira, F. A., Todd, M., Ronketti, F., Lima, S. F., Mattos, A. C. A., Reis, K. T., Coelho, P. M. Z., Thornhill, J. A. and Ribeiro, F. (2006). The effects of drugs, ions, and poly-l-lysine on the excretory system of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz 1, (Suppl.) S293–S298.CrossRefGoogle Scholar
Lima, S. F., Vieira, L. Q., Harder, A. and Kusel, J. R. (1994). Altered behavior of carbohydrate-bound molecules and lipids in areas of the tegument of adult Schistosoma mansoni worms damaged by praziquantel. Parasitology 109, 469–477.CrossRefGoogle Scholar
Mattos, A. C., Kusel, J. R., Pimenta, P. F. and Coelho, P. M. Z. (2006). Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz 1, (Suppl.) S283–S287.CrossRefGoogle Scholar
Mehlhorn, H., Becker, B., Andrews, P., Thomas, H. and Frenkel, J. K. (1981). In vivo and in vitro experiments on the effects of praziquantel on Schistosoma mansoni. Arzneimittelforschung 31, 544–554.Google ScholarPubMed
Messerli, S. M., Kasinathan, R. S., Morgan, W., Spranger, S. and Greenberg, R. M. (2009). Schistosoma mansoni P-glycoprotein levels increase in response to praziquantel exposure and correlate with reduced praziquantel susceptibility. Molecular and Biochemical Parasitology 167, 54–59.CrossRefGoogle ScholarPubMed
Modha, J., Lambertucci, J. R., Doenhoff, M. J. and McLaren, D. J. (1990). Immune dependence of schistosomicidal chemoterapy: an ultraestructural study of Schistosoma mansoni adult worms exposed to praziquantel and immune serum in vivo. Parasite Immunology 12, 321–334.CrossRefGoogle Scholar
Oliveira, F. A., Kusel, J. R., Ribeiro, F. and Coelho, P. M. Z. (2006). Responses of the surface membrane and excretory system of Schistosoma mansoni to damage and to treatment with praziquantel and other biomolecules. Parasitology 132, 321–330.CrossRefGoogle ScholarPubMed
Pax, R. A., Bennett, J. L. and Fetterer, R. (1978). A benzodiazepine derivative and praziquantel: effects on musculature of Schistosoma mansoni and Schistosoma japonicum. Naunyn-Schmiedeberg's Archives of Pharmacology 304, 309–315.Google Scholar
Pellegrino, J. and Katz, N. (1968). Experimental chemotherapy of Schistosoma mansoni. Advances in Parasitology 6, 233–291.CrossRefGoogle Scholar
Ribeiro, F., Coelho, P. M. Z., Vieira, L. Q., Watson, D. G. and Kusel, J. R. (1998). The effect of praziquantel treatment on glutathione concentration in Schistosoma mansoni. Parasitology 116, 229–236.CrossRefGoogle ScholarPubMed
Sato, H., Kusel, J. R. and Thornhill, J. A. (2002). Functional visualization of the excretory system of adult Schistosoma mansoni by the fluorescent marker resorufin. Parasitology 125, 527–535.CrossRefGoogle ScholarPubMed
Sato, H., Kusel, J. R. and Thornhill, J. A. (2004). Excretion of fluorescent substrates of mammalian multidrug resistance associated protein (MRP) in the Schistosoma mansoni excretory system. Parasitology 128, 43–52.Google Scholar
Schinkel, A. H. and Jonker, J. W. (2003). Mammalian drug efflux transporters of the ATP binding cassette (ABC) family: an overview. Advanced Drug Delivery Reviews 55, 3–29.Google Scholar
Smithers, S. R. and Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology 55, 695–700.Google Scholar
Thornhill, J. R., Kusel, J. R., Oliveira, F. A., Ribeiro, F., Lima, S. F., Coelho, P. M. Z., Meveigh, P. and Mattos, A. C. A. (2009). The uptake of macromolecules by cercariae during skin penetration and during in vitro transformation to schistosomula (Schistosoma mansoni). Memórias do Instituto Oswaldo Cruz 105 (in the Press).Google Scholar
William, S., Botros, S., Ismail, M., Farghally, A., Day, T. A. and Bennett, J. L. (2001). Praziquantel-induced tegumental damage in vitro is diminished in schistosomes derived from praziquantel-resistant infections. Parasitology 122, 63–66.CrossRefGoogle ScholarPubMed
World Health Organization. (2002). TDR Strategic Direction for Research: Schistosomiasis, World Health Organization, Geneva, Switzerland.Google Scholar