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Molecular cytology identification of 22 sugarcane germplasm clones from Sri Lanka

Published online by Cambridge University Press:  15 May 2023

Xujuan Li Open the ORCID record for Xujuan Li [Opens in a new window] ,
Jun Mao ,
Xuekuan Chen ,
Xiuqin Lin ,
Xiaoyan Wang ,
Chaohua Xu ,
Xinlong Liu  and
Xin Lu
Xujuan Li
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
Jun Mao
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
Xuekuan Chen
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
Xiuqin Lin
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
Xiaoyan Wang
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
Chaohua Xu
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
Xinlong Liu
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
Xin Lu*
Affiliation:
Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
*
Corresponding author: Xin Lu; Email: xinlu_ky@126.com
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Abstract

Germplasm innovation can provide materials for breeding sugarcane cultivars. Saccharum officinarum is the main source of high-sugar and high-yield genes in sugarcane breeding. ‘Nobilization’ is the theoretical basis for exploiting S. officinarum, and S. officinarum authenticity directly affects sugarcane nobility breeding efficiency. Herein, the authenticity of 22 SLC-series S. officinarum clones imported from Sri Lanka and preserved in the China National Germplasm Repository of Sugarcane (NGRS) was explored by four-primer amplification-arrested mutation PCR (ARMS PCR) and somatic chromosome number counting. The amplified bands from SLC 08 120 and SLC 08 131 were the same with those from S. officinarum clone Badila, i.e. a common band of 428 bp and a S. officinarum-specific band of 278 bp, hence they were tentatively assigned as S. officinarum clones. The other 20 SLC clones had both 278 bp (S. officinarum-specific) and 203 bp (S. spontaneum-specific) bands, which are hybrid characteristics. In addition, the chromosome numbers of SLC 08 120 and SLC 08 131 are both 80, belong to typical S. officinarum. While the chromosome numbers of the other 20 materials are ranging from 101 to 129, consistent with hybrids of S. officinarum and S. spontaneum. This molecular cytological characterization indicates that among the 22 introduced SLC-series clones, only two, SLC 08 120 and SLC 08 131, were S. officinarum. Future agronomic trait and resistance analyses could facilitate their use as crossing parents in sugarcane breeding.

Keywords

ARMS PCRchromosome number countSaccharum officinarum LSaccharum spontaneum Lsugarcane breedingsugarcane germplasm
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 6 , December 2022 , pp. 377 - 382
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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Footnotes

*

These authors contributed equally to this work.

References

Amaresh, C, Grisham, MP and Pan, YB (2014) Allelic divergence and cultivar-specific SSR alleles revealed by capillary electrophoresis using fluorescence-labeled SSR markers in sugarcane. Genome 57, 1–10.Google Scholar
Bakker, H (1999) Sugar Cane Cultivation and Management. New York: Springer, p. 1.CrossRefGoogle Scholar
Brookes, AJ (1999) The essence of SNPs. Gene 234, 177–186.CrossRefGoogle ScholarPubMed
Chai, J, Yu, F, Xie, SW, Huang, F, Deng, ZH and Yang, YQ (2019) Advances in molecular cytogenetics on the nobilization of sugarcane Saccharum spontaneum. Acta Agriculture Boreali Sinica 34, 386–393.Google Scholar
Chen, RK (2003) Theory and Practice of Modern Sugarcane Breeding. Beijing: China Agricultural Press, pp. 392–394.Google Scholar
D'Hont, A, Rao, PS, Feldmann, P, Grivet, L and Glaszmann, JC (1995) Identification and characterisation of sugarcane intergeneric hybrids, Saccharum officinarum x Erianthus arundinaceus, with molecular markers and DNA in situ hybridisation. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 91, 320–326.CrossRefGoogle ScholarPubMed
D'Hont, A, Grivet, L, Feldmann, P, Glaszmann, JC, Rao, S and Berding, N (1996) Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp) by molecular cytogenetics. Molecular and General Genetics Mgg 250, 405–413.CrossRefGoogle ScholarPubMed
Edmé, SJ, Glynn, NG and Comstock, JC (2006) Genetic segregation of microsatellite markers in Saccharum officinarum and S. spontaneum. Heredity 97, 366–375.CrossRefGoogle ScholarPubMed
Fischer, RA, Byerlee, D and Edmeades, G (2014) Crop yields and global food security: will yield increase continue to feed the world? Australian Centre for International Agricultural Research 6, 903–904.Google Scholar
Hermann, S, Aitken, KS, Jackson, PA, George, AW, Piperidis, N, Wei, X, Kilian, A and Detering, F (2012) Evidence for second division restitution as the basis for 2n+n maternal chromosome transmission in a sugarcane cross. Euphytica 187, 359–368.CrossRefGoogle Scholar
Hou, YP, Luo, QQ, Chen, CJ and Zhou, MG (2013) Application of tetra primer ARMS-PCR approach for detection of Fusarium gramine arum genotypes with resistance to carbendazim. Australasian Plant Pathology 42, 73–78.CrossRefGoogle Scholar
Irvine, JE (1999) Saccharum species as horticultural classes. Theoretical and Applied Genetics 98, 186–194.CrossRefGoogle Scholar
Jackson, PA (2005) Breeding for improved sugar content in sugarcane. Field Crops Research 92, 277–290.CrossRefGoogle Scholar
Liang, YL, Yu, SF, Zhen, Z, Zhao, YJ, Deng, JM and Jiang, WG (2020) Climatic change impacts on Chinese sugarcane planting: benefits and risks. Physics and Chemistry of the Earth Parts A/B/C 116, 1–8.CrossRefGoogle Scholar
Lin, XQ, Lu, X, Cai, Q, Zhou, JF, Mao, J, Wu, ZD, Liu, HB, Liu, XL, Li, XJ, Zi, QY, Li, CJ and Xu, CH (2020) An efficient method for stem tip chromosome production of Saccharum or Saccharum relatives, CN111238888A.Google Scholar
Liu, JL, Bai, CJ, Yan, LL, Jia, QL, Luo, C and Zhang, Y (2015) Genetic diversity assessment of 43 Saccharum spontaneum L. varieties with agronomic traits. Chinese Journal of Tropical Crops 36, 229–236.Google Scholar
Liu, JY, Deng, ZH, Wu, CW, Tao, LA, Lu, X, Zhao, PF and Zhang, YB (2021) Progress and discussion of sugarcane breeding using Saccharum spontaneum L. Journal of Plant Genetic Resources 22, 1491–1497.Google Scholar
Mary, S, Nair, NV, Chaturvedi, PK and Selvi, A (2006) Analysis of genetic diversity among Saccharum spontaneum L. from four geographical regions of India, using molecular marker. Genetic Resources & Crop Evolution 53, 1221–1231.CrossRefGoogle Scholar
Park, H, Kim, S, Nie, H, Kim, J, Lee, J and Kim, S (2020) Molecular identification of sweet potato accessions using ARMS-PCR based on SNPs. Journal of Plant Biotechnology 47, 124–130.CrossRefGoogle Scholar
Piperidis, G and D'Hont, A (2001) Chromosome composition analysis of various Saccharum interspecific hybrids by genomic in situ hybridisation (GISH). Australian Society of Sugar Cane Technologists 9, 565–566.Google Scholar
Piperidis, G, Piperidis, N and D'Hont, A (2010) Molecular cytogenetic investigation of chromosome composition and transmission in sugarcane. Molecular Genetics and Genomics 284, 65–73.CrossRefGoogle ScholarPubMed
Shah, SS, Jagathesan, D and Venkataraman, MR (1970) Introgression in Saccharum officinarum and Saccharum robustum. Indian Journal of Genetics and Plant Breeding 30, 668–672.Google Scholar
Sobhakumari, VP (2013) New determinations of somatic chromosome number in cultivated and wild species of Saccharum. Caryologia -Firenze 66, 268–274.CrossRefGoogle Scholar
Wang, P, Fan, Y, Huang, YJ, Chen, SY, Jing, YF and Deng, ZH (2015) Determination of authenticity of Saccharum officinarum clones by chromosome numbers. Subtropical Agriculture Research 11, 160–163.Google Scholar
Wang, HB, Xiao, NY, Zhu, ZW, Liu, CC, Alam, I, Chen, PH and Lu, H (2018) Development and characterization of SSR markers from the whole genome sequences of Saccharum officinarum (LA-purple). Acta Agronomica Sinica 44, 1400–1410.CrossRefGoogle Scholar
Yang, S, Li, XT, Huang, F, Huang, YJ, Liu, XL, Wu, JY, Wang, QN, Deng, ZH, Chen, RK and Zhang, MQ (2018) A new method based on SNP of nrDNA-ITS to identify Saccharum spontaneum and its progeny in the genus Saccharum. PLoS ONE 13, 1–12.Google ScholarPubMed
Ye, S, Dhillon, S, Ke, XY, Collins, AR and Inm, D (2001) An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Research 29, 4–8.CrossRefGoogle ScholarPubMed
Yu, F, Wang, P, Li, XT, Huang, YJ, Wang, QN, Luo, L, Jing, YF, Liu, XL, Deng, ZH, Wu, JY, Yang, YQ, Chen, RK, Zhang, MQ and Xu, LN (2018) Characterization of chromosome composition of sugarcane in nobilization by using genomic in situ hybridization. Molecular Cytogenetics 11, 35.CrossRefGoogle ScholarPubMed
Yu, JJ, Chi, ML, Jia, YY, Liu, SL, Zhu, JQ and Gu, ZM (2020) Tetra-primer amplification refractory mutation system PCR and its application in fauna and flora genetics and breeding research. Biotechnology Bulletin 36, 32–38.Google Scholar
Zhang, C, Lv, J, Han, Y, Chen, YJ, Shen, F, Wang, XJ and Shao, GJ (2015) Tetra-primer ARMS PCR and its application in rice genetics and breeding research. Liaoning Agricultural Sciences 1, 47–51.Google Scholar
Zhang, YB, Wang, LW, Lu, WX, Wu, CW, Liu, JY, Zhao, PF, Liu, XL and Huang, YK (2020) Modern Sugarcane Breeding Theory and Variety Selection-Heterogeneous Complex Resistant High Yield and High Sugar Breeding and Practice. Beijing: Science Press, p. 7.Google Scholar
Zhong, HQ, Li, FS and Yang, QH (2005) A summary on sugarcane hybrid reliability identification. Chinese Agriculture Science Bulletin 21, 390–394.Google Scholar