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Morpho-molecular exploration and selection of elite genotypes from indigenous Syzygium cumini L. Skeels (jamun) diversity of North-Western Indian Himalayas

Published online by Cambridge University Press:  19 October 2023

Vikas Kumar Sharma ,
Dheeraj Sharma ,
Rahul Sharma Open the ORCID record for Rahul Sharma [Opens in a new window] ,
Som Dev Sharma ,
Karuna Dhiman  and
Ashutosh A. Murkute
Vikas Kumar Sharma
Affiliation:
Department of Fruit Science, College of Horticulture and Forestry, Dr YSP University of Horticulture and Forestry, Neri, Hamirpur (HP) 177001, India
Dheeraj Sharma
Affiliation:
Department of Fruit Science, College of Horticulture and Forestry, Dr YSP University of Horticulture and Forestry, Neri, Hamirpur (HP) 177001, India
Rahul Sharma*
Affiliation:
Department of Fruit Science, College of Horticulture, Dr YSP University of Horticulture and Forestry, Nauni, Solan (HP) 173230, India
Som Dev Sharma
Affiliation:
Department of Fruit Science, College of Horticulture and Forestry, Dr YSP University of Horticulture and Forestry, Neri, Hamirpur (HP) 177001, India
Karuna Dhiman
Affiliation:
Department of Biotechnology, College of Horticulture and Forestry, YSP University of Horticulture and Forestry, Neri, Hamirpur (HP) 177001, India
Ashutosh A. Murkute
Affiliation:
Indian Council of Agricultural Research – Central Citrus Research Institute, Nagpur 440009, India
*
Corresponding author: Rahul Sharma; Email: himrahul50@gmail.com
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Abstract

Efficiently distinguishing various Syzygium cumini L. Skeels (jamun) accessions holds practical significance for selection purposes. This study concentrated on 15 superior genotypes of jamun from the North Western Indian Himalayas, selected for their pivotal horticultural traits. Drawn from a pool of 82 collected genotypes and assessed across two consecutive years (2019 and 2020), these genotypes underwent morphological evaluations utilizing a randomized block design replicated thrice. Concurrently, random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were employed for molecular analysis. Substantial variations surfaced among genotypes, both in morphological traits and fruit biochemistry. Notably, tree 43 exhibited promise across multiple horticultural facets, encompassing fruit weight, length, pulp weight, pulp-to-seed ratio and pulp percentage. Conversely, tree 49 excelled in elevated levels of total soluble solids, total sugar and reducing sugar. While principal component analysis and cluster analysis unveiled modest genetic variability, RAPD and ISSR markers unveiled pronounced molecular-level polymorphism. Agglomerative hierarchical clustering delineated the genotypes into five distinct clusters. Cluster I encompassed two genotypes, cluster II embraced five while the largest group, cluster III, included six genotypes. Clusters IV and V highlighted individual genotypes, trees 43 and 54 respectively. In the molecular analysis, UPGMA clustering yielded two primary clusters, spotlighting the noteworthy similarity between genotypes trees 49 and 52 whereas, trees 40, 43, 44 and 48 stood distinct. The observed genetic diversity stands as a valuable resource with substantial potential to enrich diverse breeding initiatives. These salient genetic variations underscore the richness within the studied population, offering a valuable asset for focused future pursuits.

Keywords

characterizationcluster analysisDNA markershorticultural traitsprincipal component analysis
Type
Research Article
Information
Plant Genetic Resources , Volume 21 , Issue 4 , August 2023 , pp. 312 - 322
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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