Hostname: page-component-848d4c4894-ttngx Total loading time: 0 Render date: 2024-06-09T01:20:22.039Z Has data issue: false hasContentIssue false
  • English
  • Français

Cloning and characterization of a COBRA-like gene expressed de novo during maize germination

Published online by Cambridge University Press:  22 February 2007

Felipe Cruz-García ,
Alberto Gómez ,
José Juan Zúñiga ,
Javier Plasencia  and
Jorge M. Vázquez-Ramos
Felipe Cruz-García
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
Alberto Gómez
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
José Juan Zúñiga
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
Javier Plasencia
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
Jorge M. Vázquez-Ramos*
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
*
*Correspondence Fax: +52 5622 5284, Email: jorman@servidor.unam.mx
Get access Rights & Permissions [Opens in a new window]

Abstract

The search for germination-specific genes has been a laborious and unrewarding task, since many of the genes expressed during germination are also expressed in embryogenesis or in other developmental stages. By using mRNA differential display of transcript populations from maize (Zea mays L.) embryo axes, germinated for different times with or without a previous osmopriming treatment, a 682 bp cDNA was isolated that was present only after 24 h germination, and absent during osmopriming or during early germination. Screening of a cDNA library using the 682 bp probe yielded a 1554 bp cDNA that contained an open reading frame coding for 436 amino acids. This gene, referred to as ZmAA9-24, was expressed in root tissues, but was not detected in shoot or leaf tissues. Expression of ZmAA9-24 occurred earlier during germination (by 15 h) if embryo axes were imbibed in the presence of cytokinins or if seeds were previously osmoprimed. The predicted protein sequence of ZmAA9-24 is 39.6% identical to the product of the recently identified Arabidopsis gene COBRA (54.5% in the central region), which appears to participate in the regulation of cell expansion, particularly in roots, and belongs to the glycosylphosphatidylinositol (GPI)-anchored protein family. ZmAA9-24 expression might be regulated by both cell expansion and the cell cycle, processes that have a central role during seed germination.

Keywords

ZmAA9-24 geneCOBRA genecell elongationZea mays L.primingbenzyladenine
Type
Research Article
Information
Seed Science Research , Volume 13 , Issue 3 , September 2003 , pp. 209 - 217
Copyright
Copyright © Cambridge University Press 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ausubel, F.M., Brent, R., Kingstone, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1994) Current protocols in molecular biology. New York, John Wiley & Sons.Google Scholar
Baíza, A.M., Vázquez-Ramos, J.M. and Sánchez de Jiménez, E. (1989) DNA synthesis and cell division in embryonic maize tissues during germination. Journal of Plant Physiology 135, 416–421.Google Scholar
Baker, J., Steele, C. and Dure, L. (1988) Sequence and characterization of 6 LEA proteins and their genes from cotton. Plant Molecular Biology 11, 277–291.Google Scholar
Benfey, P.N., Linstead, P.J., Roberts, K., Schiefelbein, J.W., Hauser, M.T. and Aeschbacher, R.A. (1993) Root development in Arabidopsis: four mutants with dramatically altered root morphogenesis. Development 119, 57–70.CrossRefGoogle ScholarPubMed
Bernfield, M., Götte, M., Park, P.W., Reizes, O., Fitzgerald, M.L., Lincecum, J. and Zako, M. (1999) Functions of cell surface heparan sulfate proteoglycans. Annual Review of Biochemistry 68, 729–777.Google Scholar
Bewley, J.D. and Black, M. (1994) Seeds: Physiology of development and germination. (2nd edition). New York, Plenum Press.CrossRefGoogle Scholar
Bradford, K.J. (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience 21, 1105–1112.Google Scholar
Cruz-García, F., Jiménez, L.F. and Vázquez-Ramos, J.M. (1995) Biochemical and cytological studies on osmoprimed maize seeds. Seed Science Research 5, 15–23.CrossRefGoogle Scholar
Galau, G.A., Hughes, D.W. and Dure, L. (1986) Abscisic acid induction of cloned cotton late embryogenesis abundant (LEA) mRNAs. Plant Molecular Biology 7, 155–170.Google Scholar
Heydecker, W. and Coolbear, P. (1977) Seed treatments for improved performance – survey and attempted prognosis. Seed Science and Technology 5, 353–425.Google Scholar
Jendrisak, J. (1980) The use of α-amanitin to inhibit in vivo RNA synthesis and germination in wheat embryos. Journal of Biological Chemistry 255, 8529–8533.Google Scholar
Krogh, A., Larsson, B., von Heijne, G. and Sonnhammer, E.L.L. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. Journal of Molecular Biology 305, 567–580.CrossRefGoogle ScholarPubMed
Leuchter, R., Wolf, K. and Zimmermann, M. (1998) Isolation of an. Arabidopsis thaliana cDNA complementing a Schizosaccharomyces pombe mutant deficient in phytochelatin synthesis (Accession No. AJ006787). Plant Physiology, 117, 1526Google Scholar
Liang, P. and Pardee, A.B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–971.Google Scholar
Reyes, J., Jiménez-García, L.F., González, M.A. and Vázquez-Ramos, J.M. (1991) Benzyladenine-stimulation of nuclear DNA synthesis and cell division in germinating maize. Seed Science Research 1, 113–117.Google Scholar
Sánchez de Jiménez, E. and Aguilar, R. (1984) Protein synthesis patterns. Relevance of old and new messenger RNA in germinating maize embryos. Plant Physiology 75, 231–234.CrossRefGoogle Scholar
Sánchez-de-Jiménez, E., Aguilar, R. and Dinkova, T. (1997) S6 ribosomal protein phosphorylation and translation of stored mRNA in maize. Biochimie 79, 187–194.CrossRefGoogle ScholarPubMed
Schindelman, G., Morikami, A., Jung, J., Baskin, T.I., Carpita, N.C., Derbyshire, P., McCann, M.C. and Benfey, P.N. (2001) COBRA encodes a putative GPI-anchored protein, which is polarly localized and necessary for oriented cell expansion in Arabidopsis. Genes and Development 15, 1115–1127.Google Scholar
Turnbull, J., Powell, A. and Guimond, S. (2001) Heparan sulfate: decoding a dynamic multifunctional cell regulator. Trends in Cell Biology 11, 75–81.Google Scholar
Vatamaniuk, O.K., Mari, S., Lu, Y.P., and Rea, P.A. (1999) AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution. Proceedings of the National Academy of Sciences 96, 7110–7115.Google Scholar
Vázquez-Ramos, J.M. and Reyes-Jiménez, J.R. (1990) Stimulation of DNA synthesis and DNA polymerase activity by benzyladenine during early germination of maize axes. Canadian Journal of Botany 68, 2590–2594.Google Scholar
Zúñiga-Aguilar, J.J., López, I., Gómez, A. and Vázquez-Ramos, J.M. (1995) Does benzyl adenine stimulate DNA metabolism by modifying gene expression during maize germination? Seed Science Research 5, 219–226.Google Scholar