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Electrical impedance spectroscopy in relation to seed viability and moisture content in snap bean (Phaseolus vulgaris L.)

Published online by Cambridge University Press:  22 February 2007

T. Repo ,
D.H. Paine  and
A.G. Taylor
T. Repo
Affiliation:
Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA
D.H. Paine
Affiliation:
Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA
A.G. Taylor*
Affiliation:
Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA
*
*Correspondence Fax: +13157872320 Email: agt1@cornell.edu
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Abstract

A method, electrical impedance spectroscopy (EIS), is introduced to study seed viability non-destructively. Snap bean (Phaseolus vulgaris L.) seeds were studied by EIS to determine the most sensitive EIS parameter(s) and the optimal range of moisture content (MC) for separation of viable and non-viable seeds. Hydrated seeds exhibited two impedance arcs in the complex plane at the frequency range from 60 Hz to 8 MHz, and impedance spectra of viable and non-viable seeds differed. The hydrated seeds were best-modelled by an equivalent electrical circuit with two distributed circuit elements in series with a resistor (Voigt model). Moisture content and seed viability had strong effects on the EIS parameters. The most sensitive EIS parameters for detecting the differences between viable and non-viable seeds were the capacitance log(C2), the resistance R2, the resistance ratio R2/R1 and the apex ratio, which all represent specific features of the impedance spectrum. The highest differentiation in the EIS parameters between the viable and non-viable seeds occurred in partially imbibed seeds between MC of 40 and 45% (fresh weight basis).

Keywords

bioimpedanceelectrical impedance spectroscopyseed ageingseed qualityseed testingseed viability
Type
Research Article
Information
Seed Science Research , Volume 12 , Issue 1 , March 2002 , pp. 17 - 29
Copyright
Copyright © Cambridge University Press 2002

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References

Ackmann, J.J. and Seitz, M.A. (1984) Methods of complex impedance measurements in biologic tissue. CRC Critical Review in Biomedical Engineering 11, 281–311.Google ScholarPubMed
AOSA(1983) Seed vigor testing handbook. Association of Official Seed Analysts.Google Scholar
Cox, M.A., Zhang, M.I.N. and Willison, J.H.M. (1993) Apple bruise assessment through electrical impedance measurements. Journal of Horticultural Science 68, 393–398.CrossRefGoogle Scholar
Forney, C.F. and Brandl, D.G. (1992) Control of humidity in small controlled-environment chambers using glycerol–water solutions. HortTechnology 2, 52–54.CrossRefGoogle Scholar
Glerum, C. (1980) Electrical impedance techniques in physiological studies. New Zealand Journal of Forestry Science 10, 196–207.Google Scholar
Grimnes, S. and Martinsen, Ø.G. (2000) Bioimpedance and bioelectricity basics. San Diego, Academic Press.Google Scholar
Harker, F.R. and Maindonald, J.H. (1994) Ripening of nectarine fruit. Changes in the cell wall, vacuole, and membranes detected using electrical impedance spectroscopy. Plant Physiology 106, 165–171.CrossRefGoogle Scholar
ISTA(1996) International rules for seed testing. International Seed Testing Association. Seed Science and Technology 24, supplement.Google Scholar
Jossinet, J., McAdams, E.T. and Risacher, F. (1995) The biophysical interpretation of tissue multi-frequency loci. Innovations and Technology in Biology and Medicine 16, 706–717.Google Scholar
Kučera, L.J. (1986) Kernspintomographie und elektrische Widerstandsmessung als Diagnosemethoden der Vitalität erkrankter Bäume. Schweizerische Zeitschrift für Forstwesen 137, 673–690.Google Scholar
Macdonald, J.R. (1987) Impedance spectroscopy: Emphasizing solid materials and systems. New York, John Wiley and Sons.Google Scholar
Obroucheva, N.V. and Antipova, O.V. (1997) Physiology of the initiation of seed germination. Russian Journal of Plant Physiology 44, 250–264.Google Scholar
Palta, J.P. and Weiss, L.S. (1993) Ice nucleation and freezing injury: an overview on the survival mechanisms and molecular aspects of injury and cold acclimation in herbaceous plants. pp. 143–176in Li, P.H.;, Christersson, L. (Eds) Advances in plant cold hardiness. Boca Raton, FL, CRC Press.Google Scholar
Pethig, R. and Kell, D.B. (1987) The passive electrical properties of biological systems: their significance in physiology, biophysics and biotechnology. Physics in Medicine and Biology 32, 933–970.CrossRefGoogle ScholarPubMed
Priestley, D.A. (1986) Seed aging. New York, Cornell University Press.Google Scholar
Pukacki, P. (1982) Influence of freezing damage on impedance parameters in Magnolia shoots. Arboretum Kornickie 27, 219–234.Google Scholar
Repo, T. (1994) Influence of different electrodes and tissues on the impedance spectra of Scots pine shoots. Electro- and Magnetobiology 13, 1–14.CrossRefGoogle Scholar
Repo, T. and Pulli, S. (1996) Application of impedance spectroscopy for selecting frost hardy varieties of English ryegrass. Annals of Botany 78, 605–609.CrossRefGoogle Scholar
Repo, T. and Zhang, M.I.N. (1993) Modelling woody plant tissues using a distributed electrical circuit. Journal of Experimental Botany 44, 977–982.CrossRefGoogle Scholar
Repo, T., Zhang, M.I.N., Ryyppö, A., Vapaavuori, E. and Sutinen, S. (1994) Effects of freeze–thaw injury on parameters of distributed electrical circuits of stems and needles of Scots pine seedlings at different stages of acclimation. Journal of Experimental Botany 45, 823–833.Google Scholar
Repo, T., Zhang, G., Ryyppö, A. and Rikala, R. (2000) The electrical impedance spectroscopy of Scots pine (Pinus sylvestris L.) shoots in relation to cold acclimation. Journal of Experimental Botany 51, 2095–2107.CrossRefGoogle ScholarPubMed
Ryyppö, A., Repo, T. and Vapaavuori, E. (1998) Development of freezing tolerance in roots and shoots of Scots pine seedlings at nonfreezing temperatures. Canadian Journal of Forest Research 28, 557–565.CrossRefGoogle Scholar
Smith, M.T. and Berjak, P. (1995) Deteriorative changes associated with the loss of viability of stored desiccation-tolerant and desiccation-sensitive seeds. pp. 701–746in Kigel, J.;Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Stout, D.G. (1988) Effect of cold acclimation on bulk tissue electrical impedance. II. Measurements with alfalfa and birdsfoot trefoil at nonfreezing temperatures. Plant Physiology 86, 283–287.CrossRefGoogle ScholarPubMed
Tattar, T.A., Shigo, A.L. and Chase, T. (1972) Relationship between the degree of resistance to a pulsed electric current and wood in progressive stages of discoloration and decay in living trees. Canadian Journal of Forest Research 2, 236–243.CrossRefGoogle Scholar
Taylor, A.G. (1997) Seed storage, germination and quality. pp. 1–36in Wien, H.C. (Ed.) The physiology of vegetable crops. Wallingford, CAB International.Google Scholar
Taylor, A.G., Beresniewicz, M. M. and Goffinet, M. C. (1997) Semipermeable layer in seeds. pp. 429–436in Ellis, R.H.;, Black, M.;, Murdoch, A.J.;, Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Varlan, A.R. and Sansen, W. (1996) Nondestructive electrical impedance analysis in fruit: Normal ripening and injuries characterization. Electro- and Magnetobiology 15, 213–227.CrossRefGoogle Scholar
Vertucci, C.W. and Farrant, J.M. (1995) Acquisition and loss of desiccation tolerance. pp. 237–271in Kigel, J.;, Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Zhang, M.I.N. and Willison, J.H.M. (1991) Electrical impedance analysis in plant tissues: A double shell model. Journal of Experimental Botany 42, 1465–1475.CrossRefGoogle Scholar
Zhang, M.I.N. and Willison, J.H.M. (1992) Electrical impedance analysis in plant tissues: The effect of freeze–thaw injury on the electrical properties of potato tuber and carrot root tissues. Canadian Journal of Plant Science 72, 545–553.CrossRefGoogle Scholar
Zhang, M.I.N., Willison, J.H.M., Cox, M.A. and Hall, S.A. (1993) Measurement of heat injury in plant tissue by using electrical impedance analysis. Canadian Journal of Botany 71, 1605–1611.CrossRefGoogle Scholar