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Histological aspects of avocado embryo development and effect of developmental stages on germination

Published online by Cambridge University Press:  22 February 2007

R. Perán-Quesada ,
C. Sánchez-Romero ,
F. Pliego-Alfaro  and
A. Barceló-Muñoz
R. Perán-Quesada
Affiliation:
IFAPA, CIFA Churriana-Málaga, Cortijo de la Cruz s/n. 29 140 Churriana, Málaga, Spain
C. Sánchez-Romero
Affiliation:
IFAPA, CIFA Churriana-Málaga, Cortijo de la Cruz s/n. 29 140 Churriana, Málaga, Spain
F. Pliego-Alfaro*
Affiliation:
Dpto. Biología Vegetal, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, 29071, Málaga, Spain
A. Barceló-Muñoz
Affiliation:
IFAPA, CIFA Churriana-Málaga, Cortijo de la Cruz s/n. 29 140 Churriana, Málaga, Spain
*
*Correspondence: Fax: 34-952-131944 Email: ferpliego@uma.es
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Abstract

Anatomical differentiation and storage product accumulation were studied during avocado (Persea americana Mill.) zygotic embryo development using histological techniques. The results were correlated with the germination capacity under in vitro conditions. The histodifferentiation phase, characterized by active cell division and differentiation, was accomplished by 100 d after pollination (DAP), when embryos reached 16–18 mm in length. After a phase in which there were no morphological changes, the maturation phase commenced at 125 DAP in embryos 24–26 mm in length. Accumulation of starch granules and the appearance of protein bodies occurred at this time. Germination was significantly influenced by the embryo developmental stage. Different parameters used as indicators of germination quality (germination percentage, germination speed and recovery of complete plantlets) showed a positive correlation with the age of the embryos. However, germination in vitro was not achieved fully until the end of zygotic embryo development, when the embryo reached physiological maturity.

Keywords

germinationPersea americana Millprotein bodiesreserve productsstarch granuleszygotic embryogenesis
Type
Research Article
Information
Seed Science Research , Volume 15 , Issue 2 , June 2005 , pp. 125 - 132
Copyright
Copyright © Cambridge University Press 2005

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References

Alemanno, L., Berthouly, M., Michaux-Ferriere, N. (1997) A comparison between Theobroma cacao L. zygotic embryogenesis and somatic embryogenesis from floral explants. In Vitro Cellular and Developmental Biology – Plant 33, 163–172.CrossRefGoogle Scholar
Arbeloa, A. (1986) Estudio de la biología floral y fructificación en melocotonero (Prunus persica L. Batsch.). PhD Thesis, University of Navarra.Google Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1987) The development of desiccation tolerance and maximum seed quality during seed maturation in six grain legumes. Annals of Botany 59, 23–29.CrossRefGoogle Scholar
Fisher, D.B. (1968) Protein staining of ribboned epon sections for light microscopy. Histochemie 16, 92–96.CrossRefGoogle ScholarPubMed
Gerlach, D. (1969) A rapid safranin–crystal violet–light green staining sequence for paraffin sections of plant materials. Stain Technology 44, 210–211.Google ScholarPubMed
Harada, J.J. (1997) Seed maturation and control of dormancy. pp. 545–592. in Larkins, B.A.;, Vasil, I.K. (Eds) Cellular and molecular biology of plant seed development. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Heo, K., Van der Werff, H. and Tobe, H. (1998) Embryology and relationships of Lauraceae (Laurales). Botanical Journal of the Linnean Society 126, 295–322.CrossRefGoogle Scholar
Herrero, M. (1979) Cytophysiology of pollen–pistil intraspecific incompatibility in Petunia hybrida. PhD Thesis, University of Reading.Google Scholar
Jensen, W.A. (1962) Botanical histochemistry. Principles and practice. San Francisco, W.H. Freeman.Google Scholar
Johansen, D.A. (1940) Plant microtechnique, New York, McGraw-Hill.Google Scholar
Krasowski, M.J. and Owens, J.N. (1993) Ultrastructural and histochemical postfertilization megagametophyte and zygotic embryo development of white spruce (Picea glauca) emphasizing the deposition of seed storage products. Canadian Journal of Botany 71, 98–112.CrossRefGoogle Scholar
Merkle, S.A., Parrott, W.A. and Flinn, B.S. (1995) Morphogenic aspects of somatic embryogenesis. pp. 155–203. in Thorpe, T.A. (Ed.) In vitro embryogenesis in plants. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Motto, M., Thompson, R. and Salamini, F. (1997) Genetic regulation of carbohydrate and protein accumulation in seeds. pp. 479–522. in Larkins, B.A.;, Vasil, I.K. (Ed.) Cellular and molecular biology of plant seed development. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Müntz, K. (1996) Proteases and proteolytic cleavage of storage proteins in developing and germinating dicotyledonous seeds. Journal of Experimental Botany 47, 605–622.CrossRefGoogle Scholar
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.CrossRefGoogle Scholar
Pence, V.C. (1992) Abscisic acid and the maturation of cacao embryos in vitro. Plant Physiology 98, 1391–1395.CrossRefGoogle ScholarPubMed
Perán-Quesada, R. (2001) Embriogenesis in vitro de aguacate ( Persea americana Mill.). PhD Thesis, University of Málaga.Google Scholar
Pliego-Alfaro, F., Litz, R.E., Moon, P.A. and Gray, D.J. (1996) Effect of abscisic acid, osmolarity and temperature on in vitro development of recalcitrant mango nucellar embryos. Plant Cell, Tissue and Organ Culture 44, 53–61.CrossRefGoogle Scholar
Ross, J.H.E. and Murphy, D.J. (1992) Biosynthesis and localization of storage proteins, oleosins and lipids during seed development in Coriandrum sativum and other Umbelliferae. Plant Science 86, 59–70.CrossRefGoogle Scholar
Sánchez-Romero, C., Perán-Quesada, R., Barceló-Muñoz, A., Pliego-Alfaro, F. (2002) Variations in storage protein and carbohydrate levels during development of avocado zygotic embryos. Plant Physiology and Biochemistry 40, 1043–1049.CrossRefGoogle Scholar
Schroeder, C.A. (1952) Floral development, sporogenesis, and embryology in the avocado, Persea americana. Botanical Gazette 113, 270–278.CrossRefGoogle Scholar
Skene, K.G.M. and Barlass, M. (1983) In vitro culture of abscissed immature avocado embryos. Annals of Botany 52, 667–672.CrossRefGoogle Scholar
Sokal, R.R. and Rohlf, F.J. (2003) Biometry, New York, W.H. Freeman.Google Scholar
Tewes, A., Manteuffel, R., Adler, K., Weber, E. and Wobus, U. (1991) Long-term cultures of barley synthesize and correctly deposit seed storage proteins. Plant Cell Reports 10, 467–470.CrossRefGoogle ScholarPubMed
Thorpe, T.A. (1993) In vitro organogenesis and somatic embryogenesis: Physiological and biochemical aspects. pp. 19–38. in Roubelakis-Angelakis, K.A.;, Tran Thanh Van, K. (Eds) Morphogenesis in plants. New York, Plenum.CrossRefGoogle Scholar
Tomer, E. and Gazit, S. (1979) Early stages in avocado (Persea americana Mill.) fruit development: Anatomical aspects. Botanical Gazette 140, 304–309.CrossRefGoogle Scholar
Tukey, H.B. (1938) Growth patterns of plants developed from immature embryos in artificial culture. Botanical Gazette 99, 630–665.CrossRefGoogle Scholar
Whiley, A.W. (1992) Persea americana Miller. pp. 249–254. in Verheij, E.W.M.;, Coronel, R.E.Plant resources of South-East Asia: 2. Edible fruits and nuts. Wageningen, Pudoc DLO.Google Scholar