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Differential expression of three T-type calcium channels in retinal bipolar cells in rats

Published online by Cambridge University Press:  01 March 2009

CAIPING HU ,
ANDING BI  and
ZHUO-HUA PAN
CAIPING HU
Affiliation:
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan
ANDING BI
Affiliation:
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan
ZHUO-HUA PAN*
Affiliation:
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan
*
†Address correspondence and reprint requests to: Zhuo-Hua Pan, Department of Anatomy and Cell Biology, Wayne State University School of Medicine, 540 E. Canfield Avenue, Detroit, MI 48201. E-mail: zhpan@med.wayne.edu
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Abstract

Retinal bipolar cells convey visual information from photoreceptors to retinal third-order neurons, amacrine and ganglion cells, with graded potentials through diversified cell types. To understand the possible role of voltage-dependent T-type Ca2+ currents in retinal bipolar cells, we investigated the pharmacological and biophysical properties of T-type Ca2+ currents in acutely dissociated retinal cone bipolar cells from rats using whole-cell patch-clamp recordings. We observed a broad group of cone bipolar cells with prominent T-type Ca2+ currents (T-rich) and another group with prominent L-type Ca2+ currents (L-rich). Based on the pharmacological and biophysical properties of the T-type Ca2+ currents, T-rich cone bipolar cells could be divided into three subgroups. Each subgroup appeared to express a single dominant T-type Ca2+ channel subunit. The T-type calcium currents could generate low-threshold regenerative potentials or spikes. Our results suggest that T-type Ca2+ channels may play an active and distinct signaling role in second-order neurons of the visual system, in contrast to the common signaling by L-rich bipolar cells.

Keywords

T-type calcium channelsCone bipolar cellsRetinaRodentsPatch clamp
Type
Research Articles
Information
Visual Neuroscience , Volume 26 , Issue 2 , March 2009 , pp. 177 - 187
Copyright
Copyright © Cambridge University Press 2009

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