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Bioassay of Photosynthetic Inhibitors in Water and Aqueous Soil Extracts with Eurasian Watermilfoil (Myriophyllum spicatum)

Published online by Cambridge University Press:  12 June 2017

Salah A. Selim ,
Steven W. O'Neal ,
Merrill A. Ross  and
Carole A. Lembi
Salah A. Selim
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., W. Lafayette, IN 47907
Steven W. O'Neal
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., W. Lafayette, IN 47907
Merrill A. Ross
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., W. Lafayette, IN 47907
Carole A. Lembi
Affiliation:
Dep. Bot. and Plant Pathol., Purdue Univ., W. Lafayette, IN 47907
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Abstract

Eurasian watermilfoil, an aquatic flowering plant, was found to be a suitable bioassay plant for the detection of photosynthetic inhibitor herbicides in water and aqueous extracts of soil. Stock cultures of Eurasian watermilfoil were maintained in an algal-free medium under constant environmental conditions. Oxygen evolution from three lateral shoots 3 to 5 cm in length was measured before and after herbicide treatment. Inhibition of photosynthesis was detected within 10 min of treatment. Concentrations as low as 5.9 × 10–8M terbacil and 6.4 × 10–8M diuron were detected in water. Simazine, atrazine, and metribuzin were detected in water at a concentration of 10–7M. Eurasian watermilfoil bioassays in lake water spiked with various concentrations of terbacil, metribuzin, atrazine, and simazine indicated that the bioresidual activity of these chemicals can be estimated in samples of natural water without extraction or purification. Using aqueous extracts taken from herbicide-treated soil, the Eurasian watermilfoil bioassay detected minimum levels of 0.047 and 0.07 kg/ha terbacil and atrazine, respectively. Neither soybean nor oat showed visible injury symptoms at these concentrations. The Eurasian watermilfoil bioassay has the advantage of being more rapid and/or more sensitive than methods using algae, oat seedlings, or leaf discs.

Keywords

Atrazine, 6-chloro-N-ethyl-N′-(1-methylethyl)-1,3,5-triazin-2,4-diamine, diuron, N-(3,4-dichlorophenyl)-N,N-dimethylureametribuzin, 4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-onesimazine, 6-chloro-N,N-diethyl-1,3,5-triazine-2,4-diamineterbacil, 5-chloro- 3- (1,1 - dimethylethyl) − 6 - methyl-2,4(1H,3H)-pyrimidinedioneEurasian watermilfoil, Myriophyllum spicatum L. ♯3 MYPSPsoybeans, Glycine max (L.)Merr. ‘Century’oat, Avena sativa L. ‘Noble’Oxygen electrodesoil residueswater qualityatrazinediuronmetribuzinsimazineterbacilGlycine maxAvena sativaMYPSP
Type
Soil, Air, and Water
Information
Weed Science , Volume 37 , Issue 6 , November 1989 , pp. 810 - 814
Copyright
Copyright © 1989 by the Weed Science Society of America 

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References

Literature Cited

1. Bennett, P. H. and deBeer, P. R. 1984. A rapid quantitative bioassay for the determination of biologically available bromacil in soils. Pestic. Sci. 15:425–430.Google Scholar
2. Cho, K. Y., Tchan, Y. T., and Lo, E.H.M. 1972. Resistance of Chlorella to monuron, a herbicide inhibiting photosynthesis. Soil Biol. 16:18–21.Google Scholar
3. Finney, D. I. 1971. Probit Analysis: A Statistical Treatment of the Sigmoid Response Curve. 3rd ed. Cambridge Univ. Press. 318 pp.Google Scholar
4. Good, N. F. 1961. Inhibitors of the Hill reaction. Plant Physiol. 36:788–803.Google Scholar
5. Gramlich, J. V. and Frans, R. E. 1964. Kinetics of Chlorella inhibition by herbicides. Weeds 12:184–189.CrossRefGoogle Scholar
6. Helling, C. S. and Turner, B. C. 1968. Pesticide mobility: determination by soil thin-layer chromatography. Science 162:562–563.Google Scholar
7. Izawa, S. and Good, N. F. 1965. The number of sites sensitive to 3-(3,4-dichlorophenyl)-1,1-dimethyl urea, 3-(4-chlorophenyl)-1,1-dimethylurea, and 2-chloro-4-(2-propylamino)-6-ethylamino-5-triazine in isolated chloroplasts. Biochim. Biophys. Acta 102:20–38.Google Scholar
8. Kratky, B. A. and Warren, G. F. 1971. The use of three simple, rapid bioassays on forty-two herbicides. Weed Res. 11:257–262.Google Scholar
9. Marriage, P. B. 1975. Detection of triazine and urea herbicide residues by various characteristics of oat seedling in bioassays. Weed Res. 15:291–298.CrossRefGoogle Scholar
10. O'Neal, S. W. and Lembi, C. A. 1983. Effect of simazine on photosynthesis and growth of filamentous algae. Weed Sci. 31:899–903.Google Scholar
11. Parker, C. 1965. A rapid bioassay method for the detection of herbicides which inhibit photosynthesis. Weed Res. 5:181–184.Google Scholar
12. Pillay, A. R. and Tchan, Y. T. 1972. Study of soil algae. VII. Adsorption of herbicides in soil and prediction of their rate of application by algal methods. Plant Soil 36:571–594.Google Scholar
13. Schmidt, C. 1983. Actual standard and further development of an algal fluorescence bioassay. Ecotoxicol. Env. Safety 7:276–283.Google Scholar
14. Sculthorpe, C. D. 1971. The Biology of Aquatic Vascular Plants. Edward Arnold Publ., London. 610 pp.Google Scholar
15. Shaw, D. R., Peeper, T. F., and Nafziger, D. L. 1985. Comparison of chlorophyll fluorescence and fresh weight as herbicide bioassay techniques. Weed Sci. 33:29–33.Google Scholar
16. Sheets, T. J., Crafts, A. S., and Drever, H. R. 1962. Influence of soil properties on the phytotoxicity of the s-triazine herbicides. J. Agric. Food Chem. 10:458–462.Google Scholar
17. Stalder, L. and Pestemer, W. 1980. Availability to plants of herbicide residues in soil. I. A rapid method for estimating potentially available residues of herbicides. Weed Res. 20:341–347.Google Scholar
18. Tchan, Y. T., Roseby, E., and Funnel, G. R. 1975. A new rapid specific bioassay method for photosynthetic inhibiting herbicides. Soil Biol. Biochem. 7:39–44.Google Scholar
19. Tischer, W. and Strotmann, H. 1977. Relationship between inhibitor binding by chloroplasts and inhibition of photosynthetic electron transport. Biochim. Biophys. Acta 460:113–125.Google Scholar
20. Truelove, B., Davis, D. E., and Jones, L. R. 1974. A new method for detecting photosynthetic inhibitors. Weed Sci. 22:15–17.Google Scholar