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Confirmation and characterization of cyhalofop-butyl–resistant Chinese sprangletop (Leptochloa chinensis) populations from China

Published online by Cambridge University Press:  14 February 2020

Yajun Peng ,
Lang Pan ,
Ducai Liu ,
Xiaomei Cheng ,
Guolan Ma ,
Sifu Li ,
Xueyuan Liu ,
Lifeng Wang Open the ORCID record for Lifeng Wang [Opens in a new window]  and
Lianyang Bai
Yajun Peng
Affiliation:
Graduate Student, College of Plant Protection, Hunan Agricultural University, Changsha, China Research Associate, Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, China
Lang Pan
Affiliation:
Associate Professor, College of Plant Protection, Hunan Agricultural University, Changsha, China
Ducai Liu
Affiliation:
Professor, Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, China
Xiaomei Cheng
Affiliation:
Research Associate, Institute of Horticulture, Hunan Academy of Agricultural Sciences, Changsha, China
Guolan Ma
Affiliation:
Associate Professor, Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, China
Sifu Li
Affiliation:
Research Associate, Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, China
Xueyuan Liu
Affiliation:
Professor, Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, China
Lifeng Wang*
Affiliation:
Associate Professor, Agricultural Biotechnology Research Institute, Hunan Academy of Agricultural Sciences, Changsha, China
Lianyang Bai*
Affiliation:
Professor, Hunan Academy of Agricultural Sciences, Changsha, China
*
Authors for correspondence: Lifeng Wang, Agricultural Biotechnology Research Institute, Hunan Academy of Agricultural Sciences, No. 2, Yuanda Road, Furong District, Changsha, China Email: ifwang@hunaas.cn; Lianyang Bai, Hunan Academy of Agricultural Sciences, No. 2, Yuanda Road, Furong District, Changsha, China. Email: bailianyang2005@aliyun.com
Authors for correspondence: Lifeng Wang, Agricultural Biotechnology Research Institute, Hunan Academy of Agricultural Sciences, No. 2, Yuanda Road, Furong District, Changsha, China Email: ifwang@hunaas.cn; Lianyang Bai, Hunan Academy of Agricultural Sciences, No. 2, Yuanda Road, Furong District, Changsha, China. Email: bailianyang2005@aliyun.com
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Abstract

Chinese sprangletop [Leptochloa chinensis (L.) Nees] is one grass weed severely affecting rice (Oryza sativa L.) growth in paddies in China. Cyhalofop-butyl is the main herbicide used to control grass weeds in Chinese paddy fields, especially for controlling L. chinensis; however, L. chinensis has evolved resistance to cyhalofop-butyl due to continuous and extensive application. To investigate cyhalofop-butyl resistance levels and mechanisms in L. chinensis in some of the Chinese rice areas, 66 field populations were collected and treated with cyhalofop-butyl. Of these tested populations, 10 showed a high level of resistance to cyhalofop-butyl; the 50% effective dose ranged within 108.4 to 1,443.5 g ai ha−1 with resistance index values of 9.1 to 121.8 when compared with the susceptible population. Acetyl-coenzyme A carboxylase genes (ACCase) of susceptible and all 10 resistant populations were amplified and sequenced. Among them, Ile-1781-Leu, Trp-2027-Cys, Trp-2027-Ser, and Ile-2041-Asn mutations were found in five resistant populations. No known resistance-related mutations were found in the other five resistant populations, indicating that resistance to cyhalofop-butyl in these populations was likely to be endowed by non–target site resistance mechanisms. Notably, the Ile-1781-Leu and Trp-2027-Cys substitutions have previously been reported, but this is the first report of Trp-2027-Ser and Ile-2041-Asn mutations in L. chinensis. Furthermore, three derived cleaved amplified polymorphic sequence methods were developed to rapidly detect these mutations in L. chinensis.

Keywords

ACCaseamino acid substitutiondCAPSresistance mechanism
Type
Research Article
Information
Weed Science , Volume 68 , Issue 3 , May 2020 , pp. 253 - 259
Copyright
© Weed Science Society of America, 2020

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Footnotes

Associate Editor: Vijay Nandula, USDA–ARS

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