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Physiological characteristics of glufosinate resistance in rice

Published online by Cambridge University Press:  20 January 2017

Chin-Ju Tsai ,
Chang-Sheng Wang  and
Ching-Yuh Wang
Chin-Ju Tsai
Affiliation:
Department of Agronomy, National Chung-Hsing University, 250 Kuokuang Road, Taichung, Taiwan
Chang-Sheng Wang
Affiliation:
Department of Agronomy, National Chung-Hsing University, 250 Kuokuang Road, Taichung, Taiwan
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Abstract

The physiological basis of glufosinate resistance for two resistant (R) rice mutants, lines ‘R11-2’ and ‘R11-3’, was studied. Seven days after the application of 0.54 mM glufosinate, two susceptible (S) lines, i.e., variety (var.) ‘FSK’ and its inbred line ‘FSK-3’, and a reference var. Tainung 67 (TNG 67) suffered severe injury, whereas the two R lines exhibited resistance. Dose–response analysis and survival rate 14 d after treatment with 1.5 mM glufosinate also supported this observation. A 14C-glufosinate experiment showed that more labeled herbicide was absorbed by leaves of R11-2 than S lines 48 h after treatment (HAT), but the partitioning of absorbed glufosinate to each part of the shoot did not differ between R and S lines. Although a higher degradation of glufosinate in R line R11-2 was found as compared with the two S lines, i.e., 46% vs. 38 to 40%, the actual concentration of glufosinate in R line was still higher than that in S lines. Foliar application of glufosinate resulted in less inhibition of in vivo activity of glutamine synthetase (GS; EC 6.3.1.2) as well as a lower accumulation of ammonium 24 HAT in R line than in S lines. Further kinetic study of GS showed that cytosolic GS in line R11-2, with a higher enzyme-inhibition constant (Ki) value to glufosinate, was less sensitive to the toxic action of this herbicide. Therefore, a higher metabolism of, and more important, a lower susceptibility of, the target protein GS to this herbicide are suggested to contribute significantly to glufosinate resistance in these rice lines.

Keywords

Glufosinaterice, Oryza sativa L.GS activitymetabolismtranslocationresistance mechanism
Type
Research Article
Information
Weed Science , Volume 54 , Issue 4 , August 2006 , pp. 634 - 640
Copyright
Copyright © Weed Science Society of America 

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