Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-06-02T21:50:16.924Z Has data issue: false hasContentIssue false
  • English
  • Français

Confirmation of Glyphosate-Resistant Kochia (Kochia scoparia) from Sugar Beet Fields in Idaho and Oregon

Published online by Cambridge University Press:  02 November 2017

Vipan Kumar ,
Joel Felix ,
Don Morishita  and
Prashant Jha
Vipan Kumar
Affiliation:
Postdoctoral Research Associate, Montana State University, Southern Agricultural Research Center, 748 Railroad Highway, Huntley, MT, USA
Joel Felix
Affiliation:
Associate Professor, Oregon State University, Malheur Agricultural Experiment Station, Ontario, OR, USA
Don Morishita
Affiliation:
Professor, University of Idaho, Kimberly Research and Extension Center, Kimberly, ID, USA
Prashant Jha*
Affiliation:
Associate Professor, Montana State University, Southern Agricultural Research Center, 748 Railroad Highway, Huntley, MT, USA.
*
Author for correspondence: Prashant Jha, Associate Professor, Montana State University, Southern Agricultural Research Center, 748 Railroad Highway, Huntley, MT, 59037. (E-mail: pjha@montana.edu)
Get access Rights & Permissions [Opens in a new window]

Abstract

Glyphosate-resistant (GR) kochia is an increasing management concern in major cropping systems of the northwestern US. In 2014, we investigated four putative GR kochia accessions (designated as ALA, VAL, WIL, DB) collected from sugar beet fields in eastern Oregon and southwestern Idaho to characterize the level of evolved glyphosate resistance and determine the relationship between the 5-enol-pyruvylshikimate-3-phospate synthase (EPSPS) gene copy number and level of glyphosate resistance. The EPSPS gene copy number was used as a molecular marker to detect GR kochia in subsequent surveys in 2015 and 2016. Based on LD50 values from a whole-plant dose-response study, the four putative GR kochia populations were 2.0- to 9.6-fold more resistant to glyphosate than the glyphosate-susceptible (GS) accession. In an in vivo leaf-disk shikimate assay, leaf disks of GS kochia plants treated with 100-μM glyphosate accumulated 2.4- to 4.0-fold higher amounts of shikimate than the GR plants. The four GR accessions had 2.7 to 9.1 relative EPSPS gene copies compared with the GS accession (<1 EPSPS gene copies), and there was a linear relationship between EPSPS gene copy number and glyphosate resistance level (LD50 values). The 2015 and 2016 GR kochia survey results indicated that about half of the collected populations from sugar beet fields in eastern Oregon had developed resistance to glyphosate whereas only one population from the Idaho collection was confirmed glyphosate resistant. This is the first confirmation of GR kochia in sugar beet fields in eastern Oregon and southwestern Idaho. Diversified weed control programs will be required to prevent further development and spread of GR kochia in sugar beet-based rotations in this region.

Keywords

Andrew Kniss, University of WyomingGlyphosatekochia, Kochia scoparia (L.) Schrad.sugar beet, Beta vulgaris L.EPSPS gene copy numberglyphosate-resistant kochiaglyphosate resistance
Type
Weed Management-Major Crops
Information
Weed Technology , Volume 32 , Issue 1 , February 2018 , pp. 27 - 33
Copyright
© Weed Science Society of America, 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baker, DV, Withrow, JR, Brown, CS Beck, KG (2010) Tumbling: use of diffuse knapweed (Centaurea diffusa) to examine an understudied dispersal mechanism. Invasive Plant Sci Manage 3:301–309 CrossRefGoogle Scholar
Beckie, H, Blackshaw, R, Hall, L Johnson, E (2016) Pollen- and seed-mediated gene flow in kochia (Kochia scoparia). Weed Sci 64:624–633 CrossRefGoogle Scholar
Beckie, HJ, Blackshaw, RE, Low, R, Hall, LM, Sauder, CA, Martin, S, Brandt, EN Shirriff, SW (2013) Glyphosate- and acetolactate synthase inhibitor–resistant kochia (Kochia scoparia) in western Canada. Weed Sci 61:310–318 Google Scholar
Carlson, AL, Luecke, JL, Khan, MFR Dexter, AG (2007) Survey of weed control and production practices on sugar beet in Minnesota and eastern North Dakota. Sugarbeet Res Ext Reports NDSU Ext Serv 38:40–64 Google Scholar
Christoffoleti, PJ, Westra, PB Moore, F (1997) Growth analysis of sulfonylurea-resistant and -susceptible kochia (Kochia scoparia). Weed Sci 45:691–695 Google Scholar
Eberlin, CV Fore, ZA (1984) Kochia biology. Weeds Today 15:5–6 Google Scholar
Forcella, F (1985) Spread of kochia in the northwestern United States. Weeds Today 16:4–6 Google Scholar
Friesen, LF, Beckie, HJ, Warwick, SI Van Acker, RC (2009) The biology of Canadian weeds. 138. Kochia scoparia (L.) Schrad. Can J Plant Sci 89:141–167 CrossRefGoogle Scholar
Gaines, TA, Barker, AL, Patterson, EL, Westra, P, Westra, EP, Wilson, RG, Jha, P, Kumar, V Kniss, AR (2016) EPSPS gene copy number and whole-plant glyphosate resistance level in Kochia scoparia . PLoS ONE 11(12):e0168295 CrossRefGoogle ScholarPubMed
Godar, AS, Stahlman, PW, Jugulam, M Dille, JA (2015) Glyphosate-resistant kochia (Kochia scoparia) in Kansas: EPSPS gene copy number in relation to resistance levels. Weed Sci 63:587–595 Google Scholar
Heap, I (2017) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed May 15, 2017Google Scholar
Jugulam, M, Niehues, K, Godar, AS, Koo, DH, Danilova, T, Friebe, B, Sehgal, S, Varanasi, VK, Wiersma, A, Westra, P, Stahlman, PW Gill, BS (2014) Tandem amplification of a chromosomal segment harboring 5-enolpyruvylshikimate-3-phosphate synthase locus confers glyphosate resistance in Kochia scoparia . Plant Physiol 166:1200–1207 CrossRefGoogle ScholarPubMed
Kemp, NJ, Taylor, EC Renner, KA (2009) Weed management in glyphosate- and glufosinate-resistant sugar beet. Weed Technol 23:416–424 Google Scholar
Kniss, AR (2010) Comparison of conventional and glyphosate-resistant sugar beet the year of commercial introduction in Wyoming. J Sugarbeet Res 47:127–134 CrossRefGoogle Scholar
Kumar, V Jha, P (2015a) Growth and reproduction of glyphosate-resistant and susceptible populations of Kochia scoparia . PLoS One 11:e0147779 Google Scholar
Kumar, V Jha, P (2015b) Influence of glyphosate timing on Kochia scoparia demographics in glyphosate-resistant sugar beet. Crop Prot 76:39–45 CrossRefGoogle Scholar
Kumar, V, Jha, P, Giacomini, D, Westra, E Westra, P (2015) Molecular basis of evolved resistance to glyphosate and acetolactate synthase-inhibitor herbicides in kochia (Kochia scoparia) accessions from Montana. Weed Sci 63:758–769 CrossRefGoogle Scholar
Kumar, V, Jha, P Reichard, N (2014) Occurrence and characterization of kochia (Kochia scoparia) accessions with resistance to glyphosate in Montana. Weed Technol 28:122–130 CrossRefGoogle Scholar
Mengistu, LW Messersmith, CG (2002) Genetic diversity of kochia. Weed Sci 50:498–503 CrossRefGoogle Scholar
Ritz, C, Baty, F, Streibig, JC Gerhard, D (2015) Dose-response analysis using R. PLoS One 10:e0146021 CrossRefGoogle ScholarPubMed
Schweizer, EE (1973) Predicting sugarbeet root losses based on kochia densities. Weed Sci 21:565–567 CrossRefGoogle Scholar
Schwinghamer, TD Van Acker, RC (2008) Emergence timing and persistence of kochia (Kochia scoparia). Weed Sci 56:37–41 CrossRefGoogle Scholar
Seefeldt, SS, Jensen, JE Fuerst, EP (1995) Log-logistic analysis of herbicide dose–response relationships. Weed Technol 9:218–227 CrossRefGoogle Scholar
Shaner, DL, Nadler-Hassar, T, Henry, WB Koger, CH (2005) A rapid in vivo shikimate accumulation assay with excised leaf discs. Weed Sci 53:769–774 CrossRefGoogle Scholar
Stallings, GP, Thill, DC, Mallory-Smith, CA Shafii, B (1995) Pollen-mediated gene flow of sulfonylurea-resistant kochia (Kochia scoparia). Weed Sci 43:95–102 CrossRefGoogle Scholar
Varanasi, VK, Godar, AS, Currie, RS, Dille, AJ, Thompson, CR, Stahlman, PW Jugulam, M (2015) Field-evolved resistance to four modes of action of herbicides in a single kochia (Kochia scoparia L. Schrad.) population. Pest Manage Sci 71:1207–1212 CrossRefGoogle Scholar
Weatherspoon, DM Schweizer, EE (1969) Competition between kochia and sugarbeets. Weed Sci 17:464–467 CrossRefGoogle Scholar
Wiersma, AT, Gaines, TA, Preston, C, Hamilton, JP, Giacomini, D, Buell, CR, Leach, JE Westra, P (2015) Gene amplification of 5-enol-pyruvylshikimate-3-phosphate synthase in glyphosate-resistant Kochia scoparia . Planta 241:463–474 CrossRefGoogle ScholarPubMed
Wilson, RG, Yonts, CD Smith, JA (2002) Influence of glyphosate and glufosinate on weed control and sugarbeet (Beta vulgaris) yield in herbicide-tolerant sugarbeet. Weed Technol 16:66–73 CrossRefGoogle Scholar