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Effect of nucleocytoplasmic ratio on the in vitro porcine embryo development after in vitro fertilization or parthenogenetic activation

Published online by Cambridge University Press:  06 October 2021

Nguyen Thi Men ,
Thanh Quang Dang-Nguyen Open the ORCID record for Thanh Quang Dang-Nguyen [Opens in a new window] ,
Tamas Somfai ,
Hiep Thi Nguyen ,
Junko Noguchi ,
Hiroyuki Kaneko  and
Kazuhiro Kikuchi
Nguyen Thi Men
Affiliation:
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki305-8602, Japan
Thanh Quang Dang-Nguyen*
Affiliation:
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki305-8602, Japan
Tamas Somfai
Affiliation:
Institute of Livestock and Grassland Science, NARO, Ibaraki305-0901, Japan
Hiep Thi Nguyen
Affiliation:
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki305-8602, Japan Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi753-8515Japan
Junko Noguchi
Affiliation:
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki305-8602, Japan
Hiroyuki Kaneko
Affiliation:
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki305-8602, Japan
Kazuhiro Kikuchi
Affiliation:
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki305-8602, Japan The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi753-8515Japan
*
Author for correspondence: T. Q. Dang-Nguyen. Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Ibaraki305–8602, Japan. E-mail: adipy36@affrc.go.jp
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Summary

This study was conducted to examine whether the nuclear to cytoplasmic (N/C) ratio had any influence on the timing of embryo compaction and blastocoel formation, as well as formation rate and quality of blastocyst. First, we produced embryos with increased N/C ratio by removal of approximately one-third of the cytoplasm and with decreased N/C ratio by doubling the oocyte cytoplasm with an enucleated oocyte. The initiation of compaction and cavitation in reduced cytoplasm group was significantly earlier (P < 0.05) compared with the control and doubled cytoplasm groups. The rate of blastocysts in the reduced cytoplasm and doubled cytoplasm groups was significantly lower (P < 0.05) compared with the control group. Blastocyst quality in terms of total cell number in the reduced cytoplasm group was significantly lower (P < 0.05) compared with the doubled cytoplasm group, but not different from the control group. Next, we produced embryos with various N/C ratios by oocyte fusion combined with cytochalasin D treatment. The onset of compaction and cavitation in the 2N/2C group (decreased N/C ratio) was significantly delayed (P < 0.05) or had the tendency to be delayed (P = 0.064), respectively, compared with the control group (2N/1C). A significantly higher rate of blastocyst was observed in the 4N/2C group compared with the 1N/1C group (P < 0.05) but not different from the remaining groups. These results demonstrated that an increase in N/C ratio caused an earlier occurrence of morula compaction and blastocyst formation in both in vitro fertilization (IVF) and parthenogenetically activated pig embryos.

Keywords

Doubled cytoplasmNucleocytoplasmic ratioPig oocyteReduced cytoplasmTime lapse
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 298 - 304
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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