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Effect of the addition of antifreeze protein type I on the quality of post-thawed domestic cat epididymal sperm

Published online by Cambridge University Press:  15 March 2023

L.P. Alcaráz ,
P.V.S. Pereira ,
T.A. Oliveira ,
L.F.L. Correia ,
E.M. Vasconcelos ,
F.Z. Brandão  and
J.M.G. Souza-Fabjan Open the ORCID record for J.M.G. Souza-Fabjan [Opens in a new window]
L.P. Alcaráz*
Affiliation:
Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil
P.V.S. Pereira
Affiliation:
Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil
T.A. Oliveira
Affiliation:
Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil
L.F.L. Correia
Affiliation:
Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil
E.M. Vasconcelos
Affiliation:
Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil
F.Z. Brandão
Affiliation:
Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil
J.M.G. Souza-Fabjan*
Affiliation:
Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil
*
Author for correspondence: L.P. Alcaráz. Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil. E-mail: leticiaaalcaraz@gmail.com; J.M.G. Souza-Fabjan. Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil. E-mail: joannavet@gmail.com
Author for correspondence: L.P. Alcaráz. Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil. E-mail: leticiaaalcaraz@gmail.com; J.M.G. Souza-Fabjan. Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, CEP 24230-340, Niterói, RJ, Brazil. E-mail: joannavet@gmail.com
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Summary

Cryopreservation of domestic cat semen is mainly performed as a model for the establishment of endangered wild feline protocols. The supplementation of antifreeze protein type I (AFP I) to cryopreservation medium has shown improvement in frozen–thawed sperm quality in other species, but its effect on cat semen has not yet been tested. This study aimed to assess the addition of AFP I to cryopreservation medium in domestic cats. Sperm was obtained from the cauda epididymis of orchiectomized cats; sperm was then pooled in Tris buffer and allocated into three treatments, according to AFP I final concentration: 0 (control), 0.1, and 0.5 µg/ml. Nine replicates were cryopreserved in a two-step protocol and subsequently thawed at 37°C for 30 s. There was no difference (P > 0.05) among the control, 0.1 and 0.5 µg/ml groups for parameters such as motility, vitality, functional membrane integrity, mature chromatin, normal morphology, and sperm binding to egg perivitelline membrane. In the 0.5 μg/ml group only, percentages of live sperm with intact acrosome and of sperm with most inactive mitochondria (DAB III) showed a significant reduction, along with a tendency (P = 0.053) to an increase in the percentage of sperm with most active mitochondria (DAB II). In conclusion, the supplementation of 0.1 and 0.5 µg/ml of AFP I did not promote consistent beneficial effects on the overall sperm cryotolerance in domestic cats.

Keywords

AFPCryoprotectantFelineSlow freezingSperm
Type
Research Article
Information
Zygote , Volume 31 , Issue 3 , June 2023 , pp. 240 - 245
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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