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Epigenetic modifications at DMRs of imprinting genes in sperm of type 2 diabetic men

Published online by Cambridge University Press:  23 May 2022

Maryam Jazayeri Open the ORCID record for Maryam Jazayeri [Opens in a new window] ,
Poopak Eftekhari-Yazdi Open the ORCID record for Poopak Eftekhari-Yazdi [Opens in a new window] ,
Mohammad Ali Sadighi Gilani ,
Mohsen Sharafi  and
Abdolhossein Shahverdi Open the ORCID record for Abdolhossein Shahverdi [Opens in a new window]
Maryam Jazayeri
Affiliation:
Department of Reproductive Biology, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran
Poopak Eftekhari-Yazdi
Affiliation:
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran
Mohammad Ali Sadighi Gilani
Affiliation:
Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Mohsen Sharafi
Affiliation:
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Abdolhossein Shahverdi*
Affiliation:
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran
*
Authors for correspondence: Abdolhossein Shahverdi. P.O. Box: 16635-148, Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. E-mail: shahverdi@royaninstitute.org
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Summary

High rates of infertility in type 2 diabetic (T2DM) men have led to attempts to understand the mechanisms involved in this process. This condition can be investigated from at least two aspects, namely sperm quality indices and epigenetic alterations. Epigenetics science encompasses the phenomena that can lead to inherited changes independently of the genetics. This study has been performed to test the hypothesis of the relationship between T2DM and the epigenetic profile of the sperm, as well as sperm quality indices. This research included 42 individuals referred to the infertility clinic of Royan Institute, Iran in 2019–2021. The study subjects were assigned to three groups: normozoospermic non-diabetic (control), normozoospermic diabetic (DN) and non-normozoospermic diabetic (D.Non-N). Sperm DNA fragmentation was evaluated using the sperm chromatin structure assay technique. The global methylation level was examined using 5-methyl cytosine antibody and the methylation status in differentially methylated regions of H19, MEST, and SNRPN was assessed using the methylation-sensitive high-resolution melting technique. The results showed that the sperm global methylation in spermatozoa of D.Non-N group was significantly reduced compared with the other two groups (P < 0.05). The MEST and H19 genes were hypomethylated in the spermatozoa of D.Non-N individuals, but the difference level was not significant for MEST. The SNRPN gene was significantly hypermethylated in these individuals (P < 0.05). The results of this study suggest that T2DM alters the methylation profile and epigenetic programming in spermatozoa of humans and that these methylation changes may ultimately influence the fertility status of men with diabetes.

Keywords

Diabetes mellitusDNA methylationEpigenetics processesGenomic imprintingSpermatozoa
Type
Research Article
Information
Zygote , Volume 30 , Issue 5 , October 2022 , pp. 638 - 647
Copyright
© Royan Institute for Reproductive Biomedicine, 2022. Published by Cambridge University Press

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