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Essential role of follicle stimulating hormone in the maintenance of caprine preantral follicle viability in vitro

Published online by Cambridge University Press:  01 May 2007

M.H.T. Matos ,
I.B. Lima-Verde ,
M.C.A. Luque ,
J.E. Maia Jr ,
J.R.V. Silva ,
J.J.H. Celestino ,
F.S. Martins ,
S.N. Báo ,
C.M. Lucci  and
J.R. Figueiredo
M.H.T. Matos*
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
I.B. Lima-Verde
Affiliation:
Faculty of Veterinary Medicine, PPGCV, Federal Rural University of Pernambuco, Recife, PE, Brazil.
M.C.A. Luque
Affiliation:
Laboratory of Electron Microscopy, Department of Cell Biology, University of Brasilia, Brasilia, DF, Brazil.
J.E. Maia Jr
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
J.R.V. Silva
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
J.J.H. Celestino
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
F.S. Martins
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
S.N. Báo
Affiliation:
Laboratory of Electron Microscopy, Department of Cell Biology, University of Brasilia, Brasilia, DF, Brazil.
C.M. Lucci
Affiliation:
Faculty of Veterinary Medicine, University of Brasilia, Brasilia, DF, Brazil.
J.R. Figueiredo
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil.
*
All correspondence to: Maria Helena T. Matos, Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza–CE–Brasil. CEP: 60740–000. Tel: +55 85 3101 9852. Fax: +55 85 3101 9840. e-mail: htmatos@yahoo.com
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Summary

The aims of the present study were to investigate the effects of follicle-stimulating hormone (FSH) on survival, activation and growth of caprine primordial follicles using histological and ultrastructural studies. Pieces of caprine ovarian cortex were cultured for 1 or 7 days in minimum essential medium (MEM – control medium) supplemented with different concentrations of FSH (0, 10, 50 or 100 ng/ml). Small fragments from non-cultured ovarian tissue and from those cultured for 1 or 7 days in a specific medium were processed for classical histology and transmission electron microscopy (TEM). Additionally, effects of FSH on oocyte and follicle diameter of cultured follicles were evaluated. The results showed that the lowest percentage of normal follicles was observed after 7 days of culture in control medium. After 1 day of culture, a higher percentage of growing follicles was observed in the medium supplemented with 50 ng/ml of FSH. In the presence of 10 and 50 ng/ml of FSH, an increase in diameter of both oocyte and follicle on day 7 of culture was observed. TEM showed ultrastructural integrity of follicles after 1 day of culture in MEM and after 7 days in MEM plus 50 ng/ml FSH, but did not confirm the integrity of those follicles cultured for 7 days in MEM. In conclusion, this study demonstrated that FSH at concentration of 50 ng/ml not only maintains the morphological integrity of 7 days cultured caprine preantral follicles, but also stimulate the activation of primordial follicles and the growth of activated follicles.

Keywords

ActivationCaprineCultureFSHPrimordial follicles
Type
Research Articles
Information
Zygote , Volume 15 , Issue 2 , May 2007 , pp. 173 - 182
Copyright
Copyright © Cambridge University Press 2007

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