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Mouse embryonic stem cells maintain differentiation potency into somatic lineage despite alternation of ploidy

Published online by Cambridge University Press:  31 March 2022

Hiroyuki Imai ,
Wataru Fujii ,
Ken Takeshi Kusakabe ,
Yasuo Kiso  and
Kiyoshi Kano Open the ORCID record for Kiyoshi Kano [Opens in a new window]
Hiroyuki Imai
Affiliation:
Laboratory of Veterinary Anatomy, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan Laboratory of Veterinary Developmental Biology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan
Wataru Fujii
Affiliation:
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Science, The University of Tokyo, Tokyo, Japan
Ken Takeshi Kusakabe
Affiliation:
Laboratory of Veterinary Anatomy, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan
Yasuo Kiso
Affiliation:
Laboratory of Veterinary Anatomy, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan
Kiyoshi Kano*
Affiliation:
Laboratory of Veterinary Developmental Biology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan
*
Author for correspondence: Kiyoshi Kano. Laboratory of Veterinary Developmental biology, Joint faculty of Veterinary Medicine, Yamaguchi University. 1677-1, Yoshida, Yamaguchi, 753-0841, Japan. Tel: +81 83 933 5883. E-mail: kanokiyo@yamaguchi-u.ac.jp
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Summary

Vertebrates, including mammals, are considered to have evolved by whole genome duplications. Although some fish have been reported to be polyploids that have undergone additional genome duplication, there have been no reports of polyploid mammals due to abnormal development after implantation. Furthermore, as the number of physiologically existing tetraploid somatic cells is small, details of the functions of these ploidy-altered cells are not fully understood. In this present study, we aimed to clarify the details of the differentiation potency of tetraploids using tetraploid embryonic stem cells. To clarify the differentiation potency, we used mouse tetraploid embryonic stem cells derived from tetraploid embryos. We presented tetraploid embryonic stem cells differentiated into neural and osteocyte lineage in vitro and tetraploid cells that contributed to various tissues of chimeric embryos ubiquitously in vivo. These results revealed that mouse embryonic stem cells maintain differentiation potency after altering the ploidy. Our results provide an important basis for the differentiation dynamics of germ layers in mammalian polyploid embryogenesis.

Keywords

DifferentiationMouse embryonic stem cellsPloidyTetraploid
Type
Research Article
Information
Zygote , Volume 30 , Issue 4 , August 2022 , pp. 480 - 486
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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