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Pum3 is dispensable for mouse oocyte maturation and embryo development in vitro

Published online by Cambridge University Press:  17 May 2023

TingTing Zhao ,
Wei Huang  and
Kaibo Lin Open the ORCID record for Kaibo Lin [Opens in a new window]
TingTing Zhao
Affiliation:
State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, China
Wei Huang*
Affiliation:
Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Hainan, 570102, China
Kaibo Lin*
Affiliation:
Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200021, China
*
Corresponding author: Kaibo Lin. Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200021, China. Email: linkb@126.com. Wei Huang. Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Hainan, 570102, China. Email: huang1213@hainmc.edu.cn
Corresponding author: Kaibo Lin. Department of Assisted Reproduction, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200021, China. Email: linkb@126.com. Wei Huang. Department of Reproductive Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Hainan, 570102, China. Email: huang1213@hainmc.edu.cn
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Summary

Pumilio3 (Pum3), an evolutionarily distant homologue of the classical RNA-binding protein PUF (PUMILIO and FBF) family member, is also involved in the process of RNA metabolism through post-transcriptional regulation. However, the functions of Pum3 in mouse oocyte maturation and preimplantation embryonic development have not been elucidated. By comparing RNA levels in different tissues, we found that Pum3 was widely expressed in multiple tissues, but moderately predominant in the ovary. Histochemical staining suggested that the PUM3 protein exhibits positive signals in oocytes, granulosa cells and theca cells of different follicle stages. Oocyte immunofluorescence results showed a slightly higher level of PUM3 protein in metaphase II compared with the germinal vesicle (GV) stage. After knockdown of Pum3 in GV oocytes using siRNA injection (siPUM3), no obvious defect was observed in the processes of GV breakdown and polar body extrusion during in vitro maturation (IVM) for the siPum3 oocytes. Compared with the control group, the siPUM3 group displayed no significant abnormality in the cleavage and blastocyst formation rate of these fertilized oocytes. Therefore, we can conclude that depletion of Pum3 does not affect mouse oocyte maturation and early embryonic development in vitro.

Keywords

Embryo developmentOocyte maturationPUF familyPum3RNA-binding protein
Type
Research Article
Information
Zygote , Volume 31 , Issue 4 , August 2023 , pp. 359 - 365
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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