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Return temperature after heat shock affects the production of tetraploids in the yellowtail tetra Astyanax altiparanae

Published online by Cambridge University Press:  24 September 2020

Leandro Freitas Martins ,
Cleonice Cristina Hilbig ,
George Shigueki Yasui ,
Paulo Sérgio Monzani ,
José Augusto Senhorini Open the ORCID record for José Augusto Senhorini [Opens in a new window] ,
Laura Satiko Okada Nakaghi  and
Nivaldo Ferreira do Nascimento Open the ORCID record for Nivaldo Ferreira do Nascimento [Opens in a new window]
Leandro Freitas Martins
Affiliation:
Federal University of Grande Dourados (UFGD), Dourados79825-070, Brazil
Cleonice Cristina Hilbig
Affiliation:
Federal University of Grande Dourados (UFGD), Dourados79825-070, Brazil
George Shigueki Yasui
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil
Paulo Sérgio Monzani
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil
José Augusto Senhorini
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP14884-900, Brazil
Laura Satiko Okada Nakaghi
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP14884-900, Brazil
Nivaldo Ferreira do Nascimento*
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP14884-900, Brazil Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil
*
Author for correspondence: Nivaldo Ferreira do Nascimento. Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil. E-mail: nivaldotec@yahoo.com.br
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Summary

The aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40°C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22°C, 26°C and 28°C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock temperature affects the parameters analyzed and, therefore, must be considered for optimization of the production of tetraploid in A. altiparanae. Those data are innovative and could be used in future studies of basic biology in this species.

Keywords

AquacultureChromosome manipulationFlow cytometryPolyploidTriploid
Type
Short Communication
Information
Zygote , Volume 29 , Issue 1 , February 2021 , pp. 82 - 86
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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