By comparing the radiocarbon age of the soils under burial mounds of known archaeological age with the surface-exposed (background) soils of the surrounding landscapes, we may evaluate the rates of humus renewal in these soils. In the cold climate of the mideastern areas of the Russian plains, the value of humus rejuvenation coefficient decreases. This shows that humus renewal is 5–10 times slower than in the warmer climate of the southern regions. Using the obtained data on the rejuvenation rate of humus substances, we can determine the age of paleosols and study the dynamics of the carbon exchange processes in the biosphere.