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RNA Interference Technology
From Basic Science to Drug Development

$84.00 (0) USD

Andrew Fire, Alla Grishok, Jason W. Myers, James E. Ferrell, Nathaniel R. Dudley, Ahmad Z. Amin, Bob Goldstein, Amy E. Pasquinelli, Anna M. Krichevsky, Shih-Chu Kao, Li-Huei Tsai, Kenneth S. Kosik, Queta Boese, William S. Marshall, Anastasia Khvorova, Yerramilli V. B. K. Subrahmanyam, Eric Lader, Ye Ding, Charles E. Lawrence, M. Sohail, G. Doran, Kathy Latham, Vince Pallotta, Lance Ford, Mike Byrom, Mehdi Banan, Po-Tsan Ku, David Brown, Ying Mao, Chris Mello, Laurence Lamarcq, Brad Scherer, Thomas Quinn, Robert Larsen, Marcia Tan, Patty Wong, Andrew Farmer, Oded Singer, Gustavo Tiscronia, Inder M. Verma, M. Sioud, Dag R. Sørrensen, Philipp Hadwiger, Hans-Peter Vornlocher, Frank Buchholz, Federico Calegari, Ralf Kittler, Wieland B. Huttner, Dimitris Bourikas, Thomas Baeriswyl, Rejina Sadhu, Esther T. Stoeckli, Karen E. Stephens, Olivier Zugasti, Nigel J. O'Neil, Patricia Kuwabara, Mark E. Drew, Shawn A. Motyka, James C. Morris, Zefeng Wang, Paul T. Englund, Greg Arndt, Rosa M. Ruiz-Vazquez, Ramachandran Vanitharani, Padmanabhan Chellappan, Claude M. Fauquet, Patrick Lu, Martin C. Woodle, Antje Ostareck-Lederer, Sandra Clauder-Münster, Rolf Thermann, Maria Polycarpou-Schwarz, Marc Gentzel, Matthias Wilm, Joe D. Lewis, Steven A. Haney, Peter Lapan, Jeff Aalfs, Chris Childs, Paul Yaworsky, Chris Miller, Neil J. Clarke, John E. Bisi, Caretha L. Creasy, Michael K. Dush, Kris J. Fisher, John M. Johnson III, Christopher J. Ring, Mark R. Edbrooke, Derek M. Dykxhoorn, Dmitry Samarsky, Margaret Taylor, Mark A. Kay, Anton P. McCaffrey, Lars Zender, Michael Peter Manns, Stefan Kubicka, L. Scherer, John Rossi, Asako Sugimoto, Michael Boutros, Marc Hild, Maria E. Ramos-Nino, Brooke T. Mossman, Howard Y. Chang, Nancy N. Wang, Jen-Tsan Chi, Makoto Miyagishi, Sahohime Matsumoto, Takashi Futami, Hideo Akashi, Krishnarao Appasani, Yasuomi Takagi, Shizuyo Sutou, Takashi Kadowaki, Ryozo Nagai, Kazunari Taira
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  • RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this 2005 overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.

    Reviews & endorsements

    "This book provides a window to the exciting new RNA interference revoluton with special emphasis on applications for biotechnology. It will provide up-to-date background for academic and industry researchers seeking to make use of this powerful research tool." Judy Lieberman, Havard Medical School

    "A comprehensive treatise on RNA intereference has long been needed. That need has now been filled with this book, which placed RNAi and its potential squarely in the context of the post-human genome era. The successful juxtaposition of the many aspects of RNAi biology and technology should serve to stimulate creative though across the many diverse disciplines affected by the RNAi revolution." Ronald K. Scheule

    "Only six years ago Fire and Mello discovered RNAi, as a funny effect in little worms. Now the whole world wants to know about it. In this book Krishnarao Appasani gets an all-star crowd of specialists to introduce RNAi: its history, biology, application in genomics research, and possible therapeutic use. Warmly recommended." Ronald Plasterk, Hubrecht Laboratories

    "This extensive collection of timely articles brings us up to date on the current research on RNAi, its science and applications. It is a must-read to learn about current status of the art. This is a rapidly advancing field and it is useful for the newcomer both in academia and in industry to obtain a panoramic view and put the current literature in perspective." Robert Weinmann, Bristol-Myers Squibb

    "This new book is very comprehensive, in many ways picking up where Hannon's RNAi manual leaves off. A good companion to Hannon." Frank Slack, Yale University

    "This book is very timely. The chapters on the basics of RNAi, siRNA and miRNAs are an excellent summary of the recent origins of this topic, and the various sections dealing with RNAi technology provide comprehensive background material. I am sure that, even with the rapid progress in RNAi, this book will be useful for both newcomers to the field wishing to get an overview and experienced researchers looking for a summary of published information." David Baulcombe, The Sainsbury Laboratory

    "The book...is an extraordinary effort to draw together the current knowledge in this field...Each section stands on its own merit and all chapters within each section are authored by leading authorities providing well written and excellent information on all topics...This book is an excellent snap-shot of the technology and its applications at this point in time and, as such, is highly recommended." - Canadian Journal of Medical Laboratory Science, Alan McNabb, BSc, ART

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    Product details

    • Date Published: May 2005
    • format: Adobe eBook Reader
    • isbn: 9780511081316
    • availability: Adobe Reader ebooks available from eBooks.com
  • Table of Contents

    Part I. Basic RNAi, siRNA and Gene Silencing Mechanisms:
    1. RNAi beginnings: overview of the pathway in C. elegans
    2. Dicer in RNAi: its roles in vivo and utility in vitro
    3. Genes required for RNAi
    4. MicroRNAs: a small contribution from worms
    5. miRNAs in the brain and the application of RNAi to neurons
    Part II. Design and Synthesis of siRNAs:
    6. Design and synthesis of small interfering RNA (siRNA)
    7. Automated design and high throughput synthesis of siRNA
    8. Rational design of siRNAs with the Sfold software
    9. Enzymatic production of siRNAS
    Part III. Vector Development and In Vivo, In Vitro and In Ovo Delivery Methods:
    10. Six methods of inducing RNAi in mammalian cells
    11. Viral delivery of siRNA
    12. siRNA delivery by lentiviral vectors: design and applications
    13. Liposomal delivery of siRNAs in mice
    14. Chemical modifications to achieve increased stability and sensitive detection of siRNA
    15. RNAi in postimplantation mouse embryos
    16. In ovo RNAi opens new possibilities for functional genomics in vertebrates
    Part IV. Gene Silencing in Model Organisms:
    17. Practical applications of RNAi in C. elegans
    18. Inducible RNAi as a forward genetic tool in Trypanosoma brucei
    19. RNAi gene silencing in yeast
    20. RNA silencing in filamentous fungi: Mucor circinelloides as a model organism
    21. RNAi and gene silencing phenomenon mediated by viral suppressors in plants
    Part V. Drug Target Validation:
    22. Delivering siRNA in vivo for functional genomics and novel therapeutics
    23. The role of RNAi in drug target validation: application to Hepatitis C
    24. RNAi in the drug discovery process
    25. RNAi: use in the drug discovery process
    Part VI. Therapeutic and Drug Development:
    26. RNAi-mediated silencing of viral gene expression and replication
    27. RNAi in drug development: practical considerations
    28. RNAi for the treatment of liver diseases
    29. RNAi applications in animal systems
    Part VII. Genome Wide RNAi and High Through-Put Analysis:
    30. High through-put RNAi-by-soaking in Caenorhabditis elegans
    31. Tools for integrative genomics: genome-Wide RNAi and expression profiling in Drosophila
    32. Microarray analysis and RNA silencing to determine genes functionally important in Mesothelioma
    33. High through-put RNAi
    34. Generation of highly specific vector-based shRNAi libraries directed against the entire human genome.

  • general resources

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    General Resources9780521205177 Colour Platespdf10877KB0 general resources general resources general resourcesgeneral resources

    These resources are provided free of charge by Cambridge University Press with permission of the author of the corresponding work, but are subject to copyright. You are permitted to view, print and download these resources for your own personal use only, provided any copyright lines on the resources are not removed or altered in any way. Any other use, including but not limited to distribution of the resources in modified form, or via electronic or other media, is strictly prohibited unless you have permission from the author of the corresponding work and provided you give appropriate acknowledgement of the source.

    If you are having problems accessing these resources please email cflack@cambridge.org

  • Editor

    Krishnarao Appasani, GeneExpression Systems, Inc., Massachusetts

    Foreword

    Andrew Fire, Stanford University, California

    Marshall Nirenberg

    Contributors

    Andrew Fire, Alla Grishok, Jason W. Myers, James E. Ferrell, Nathaniel R. Dudley, Ahmad Z. Amin, Bob Goldstein, Amy E. Pasquinelli, Anna M. Krichevsky, Shih-Chu Kao, Li-Huei Tsai, Kenneth S. Kosik, Queta Boese, William S. Marshall, Anastasia Khvorova, Yerramilli V. B. K. Subrahmanyam, Eric Lader, Ye Ding, Charles E. Lawrence, M. Sohail, G. Doran, Kathy Latham, Vince Pallotta, Lance Ford, Mike Byrom, Mehdi Banan, Po-Tsan Ku, David Brown, Ying Mao, Chris Mello, Laurence Lamarcq, Brad Scherer, Thomas Quinn, Robert Larsen, Marcia Tan, Patty Wong, Andrew Farmer, Oded Singer, Gustavo Tiscronia, Inder M. Verma, M. Sioud, Dag R. Sørrensen, Philipp Hadwiger, Hans-Peter Vornlocher, Frank Buchholz, Federico Calegari, Ralf Kittler, Wieland B. Huttner, Dimitris Bourikas, Thomas Baeriswyl, Rejina Sadhu, Esther T. Stoeckli, Karen E. Stephens, Olivier Zugasti, Nigel J. O'Neil, Patricia Kuwabara, Mark E. Drew, Shawn A. Motyka, James C. Morris, Zefeng Wang, Paul T. Englund, Greg Arndt, Rosa M. Ruiz-Vazquez, Ramachandran Vanitharani, Padmanabhan Chellappan, Claude M. Fauquet, Patrick Lu, Martin C. Woodle, Antje Ostareck-Lederer, Sandra Clauder-Münster, Rolf Thermann, Maria Polycarpou-Schwarz, Marc Gentzel, Matthias Wilm, Joe D. Lewis, Steven A. Haney, Peter Lapan, Jeff Aalfs, Chris Childs, Paul Yaworsky, Chris Miller, Neil J. Clarke, John E. Bisi, Caretha L. Creasy, Michael K. Dush, Kris J. Fisher, John M. Johnson III, Christopher J. Ring, Mark R. Edbrooke, Derek M. Dykxhoorn, Dmitry Samarsky, Margaret Taylor, Mark A. Kay, Anton P. McCaffrey, Lars Zender, Michael Peter Manns, Stefan Kubicka, L. Scherer, John Rossi, Asako Sugimoto, Michael Boutros, Marc Hild, Maria E. Ramos-Nino, Brooke T. Mossman, Howard Y. Chang, Nancy N. Wang, Jen-Tsan Chi, Makoto Miyagishi, Sahohime Matsumoto, Takashi Futami, Hideo Akashi, Krishnarao Appasani, Yasuomi Takagi, Shizuyo Sutou, Takashi Kadowaki, Ryozo Nagai, Kazunari Taira

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