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Principles and Techniques of Biochemistry and Molecular Biology

6th Edition


Keith Wilson, John Fyffe, Anwar R. Baydoun, Kay Ohlendieck, Stephen W. Paddock, Ralph Rapley, Robin Thorpe, Susan Thorpe, John Walker, Alistair Aitken, Derek B. Gordon, Robert J. Slater
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  • Date Published: March 2005
  • availability: Replaced by 9780521731676
  • format: Paperback
  • isbn: 9780521535816


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About the Authors
  • This new and expanded edition of the bestselling textbook has now been fully revised to include techniques from across the biosciences. It focuses particularly on those techniques that have led to recent advances in molecular biology and is designed to help students understand the theory and practical principles behind those methods. The book will be essential reading for all bioscience undergraduate students and pre-clinical medical students and will complement traditional undergraduate texts in biochemistry and molecular biology.

    • Discusses the principles and practical applications of all commonly used analytical techniques in the biosciences
    • Aimed specifically at undergraduates in the life sciences, and includes all techniques that they are likely to meet in their practical classes
    • Includes increased coverage of molecular biology, including bioinformatics and proteomics techniques
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    Reviews & endorsements

    "It is necessary to have a practical, up to date guide to experimental techniques at hand in a laboratory. This book belongs to such kind of texts. It covers all branches of basic as well as advanced techniques. The book represents a fundamental guidebook for work in a laboratory." --Photosynthetica

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    Product details

    • Edition: 6th Edition
    • Date Published: March 2005
    • format: Paperback
    • isbn: 9780521535816
    • length: 802 pages
    • dimensions: 248 x 175 x 36 mm
    • weight: 1.605kg
    • contains: 321 b/w illus. 5 colour illus. 66 tables
    • availability: Replaced by 9780521731676
  • Table of Contents

    List of contributors
    List of abbreviations
    1. Basic priniciples Keith Wilson and John Fyffe
    2. Cell culture techniques Anwar R. Baydoun
    3. Centrifugation Kay Ohlendieck
    4. Microscopy Stephen W. Paddock
    5. Molecular biology, bioinformatics and basic techniques Ralph Rapley
    6. Recombinant DNA and genetic analysis Ralph Rapley
    7. Immunochemical techniques Robin Thorpe and Susan Thorpe
    8. Protein structure, purification, characterisation and functional analysis John Walker
    9. Mass spectrometric techniques Alistair Aitken
    10. Electrophoretic techniques John Walker
    11. Chromatographic techniques Keith Wilson
    12. Spectroscopic techniques I: atomic and molecular electronic spectroscopy Derek B. Gordon
    13. Spectroscopic techniques II: vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields
    14. Radioisotope techniques Robert J. Slater
    15. Enzymes Keith Wilson
    16. Cell membrane receptors and cell signalling Keith Wilson.

  • Editors

    Keith Wilson, University of Hertfordshire
    Keith Wilson is Professor Emeritus of Pharmacological Biochemistry and former Head of the Department of Biosciences, Dean of the Faculty of Natural Sciences, and Director of Research at the University of Hertfordshire.

    John Walker, University of Hertfordshire
    John Walker is Professor and Head of the School of Life Sciences and also Head of the Science Training Centre at the University of Hertfordshire.


    Keith Wilson, John Fyffe, Anwar R. Baydoun, Kay Ohlendieck, Stephen W. Paddock, Ralph Rapley, Robin Thorpe, Susan Thorpe, John Walker, Alistair Aitken, Derek B. Gordon, Robert J. Slater

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