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WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction

WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction

4th Edition

Out of Print

    • Date Published: May 1999
    • availability: Unavailable - out of print November 2008
    • format: Paperback
    • isbn: 9780521645997

    Out of Print

    Unavailable - out of print November 2008
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    About the Authors
    • This definitive and essential source of reference has been thoroughly updated and revised to meet the requirements of all laboratories involved in the analysis of human semen. The book sets out the fundamental laboratory techniques that should be employed in the diagnosis of male infertility. The text includes descriptions of how to construct a conventional semen profile and provides standardized protocols for performing several optional diagnostic procedures. Previous editions of this volume have established themselves as the gold standard in the area of fertility investigation and treatment and this new edition will set new standards for the new millennium.

      • The gold standard for the examination of human semen
      • Essential reference book for investigating infertility
      • Provides full practical details, along with illustrated examples
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      Reviews & endorsements

      "This manual would be a valuable companion to both small and large medical laboratories, as well as to andrology labs...This is a good manual and I would personally recommend it to any laboratory that performs semen analysis. It would also be a good reference tool in forensic and artificial insemination laboratories." Canadian Journal of Medical Laboratory Science

      "The WHO Manual should have a prominent place on the benches of all laboratories dealing with human semen and on the shelves of clinicians dealing with subfertile couples... The new fourth edition or the 'gold book,' as I am sure it will be called, will then continue to be the benchmark for setting more rigorous standards in the future." Human Fertility

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      Product details

      • Edition: 4th Edition
      • Date Published: May 1999
      • format: Paperback
      • isbn: 9780521645997
      • length: 138 pages
      • copublisher: The World Health Organization
      • dimensions: 254 x 193 x 14 mm
      • weight: 0.428kg
      • contains: 11 b/w illus. 4 colour illus. 12 tables
      • availability: Unavailable - out of print November 2008
    • Table of Contents

      1. Introduction
      2. Collection and examination of human semen
      2A. Standard procedures
      2B. Optional tests
      2C. Research tests
      3. Sperm preparation techniques
      4. Quality control in the andrology laboratory
      5. Sperm-cervical mucus interactions
      Appendices. IA. Reference values of semen variables
      IB. Nomenclature for some semen variables
      II. Safety guidelines for the andrology laboratory
      III. Methods for detecting leucocytes
      IV. Sperm vitality techniques
      V. Papanicolau staining procedures modified for spermatozoa
      VI. Shorr staining procedure for sperm morphology
      VII. Rapid staining procedure for sperm morphology
      VIII. Immunobead test
      IX. Mixed antiglobulin reaction test (MAR test)
      X. Measurement of zinc in seminal plasma
      XI. Measurement of fructose in seminal plasma
      XII. Measurement of neutral a-glucosidase in seminal plasma
      XIII. Sample instructions and record form for semen analysis
      XIV. Computer-aided sperm analysis (CASA)
      XV. Protocols for the zona-free hamster test
      XVI. Measurement of reactive oxygen species generated by sperm suspensions
      XVII. Induced acrosome reaction assay
      XVIII. Sperm preparation techniques
      XIX. Quality control (QC) procedures
      XX. Sample record form for cervical mucus scoring and postcoital test
      XXI. Sperm-cervical mucus interaction: the capillary tube test
      XXII. Basic requirements for an andrology laboratory

    • Corporation

      World Health Organisation

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