Protecting the reproductive potential of young patients undergoing cancer therapy is increasingly important. With modern treatment protocols, 80% of patients can be expected to survive. It has been estimated that up to one in 250 young adults will be a survivor of childhood cancer in the future; infertility, however, may be a consequence. As a wide range of fertility preservation methods are increasingly offered by clinicians, this systematic and comprehensive textbook dealing with the cryobiology, technology and clinical approach to this therapy will be essential reading to infertility specialists, embryologists, oncologists, cryobiologists, ObGyns, andrologists, and urologists with an interest in fertility preservation. Fertility Cryopreservation reviews all the techniques of this increasingly important field within reproductive medicine. It covers the basic principles of pertinent cryobiology, and contains major sections on the different therapies available, written by international specialists combining experience from both academic centers and commercial industries.
Part I. Cryobiology: 1. Cryobiology: an overview Ri-Cheng Chian; 2. Suppression of ice in aqueous solutions and its application to vitrification in an ART setting Patrick Quinn; 3. Pathway for the movement of water and cryoprotectants in mouse oocytes and embryos at different stages - its relevance to cryopreservation Magosaburo Kasai and Keisuke Edashige; 4. Cryoprotectants Jason E. Swain and Gary D. Smith; Part II. Cryopreservation of Sperm and Testicular Tissue: 5. Cryopreservation of sperm: an overview Fady Shehata and Ri-Cheng Chian; 6. Sperm cryopreservation for donor program Zheng Li and Peng Xu; 7. Cryopreservation of surgically retrieved sperm Peter T. K. Chan; 8. Testicular tissue cryopreservation Ariel Revel and Javier Mejia; Part III. Cryopreservation of Embryos: 9. Cryopreservation of embryos: an overview David H. Edgar and Debra A. Gook; 10. Cryopreservation of pronuclear stage human embryos Barry Behr and Yimin Shu; 11. Cryopreservation of day two and day three embryos Yunxia Cao and Zhiguo Zhang; 12. Cryopreservation of blastocysts Yoshiharu Morimoto; 13. Aseptic vitrification of human blastocysts: protocol development and clinical application P. Vanderzwalmen, L. Grobet, Y. Prapas, P. Frias and N. Zech; Part IV. Cryopreservation of Oocytes: 14. Cryopreservation of human oocytes: an overview Ri-Cheng Chian; 15. Cryopreservation of oocytes by slow cooling Andrea Borini and Giovanni Coticchio; 16. Vitrification of human oocytes with different tools Patrick Quinn; 17. Vitrification of human oocytes using the McGill Cryoleaf Jack Yu Jen Huang, Seang Lin Tan and Ri-Cheng Chian; 18. Cryopreservation of human oocytes and embryos by direct plunging into liquid nitrogen and its aseptic approach Evgenia Isachenko, Vladimir Isachenko and Jurgen M. Weiss; 19. Vitrification of human oocytes for a donor program Elkin Lucena, Carolina Lucena and Sandra Mojica; 20. Obstetric and perinatal outcomes in pregnancies conceived following oocyte cryopreservation William Buckett and Ri-Cheng Chian; Part V. Cryopreservation of Ovarian Tissue: 21. Cryopreservation of ovarian tissue: an overview Jacques Donnez, Pascale Jadoul, Olivier Donnez, Anne-Sophie van Eyck, Jean Squifflet and Marie-Madeleine Dolmans; 22. In vitro culture of human primordial follicles Benoit Schubert and Johan Smitz; 23. Cryobanking of human ovarian tissue: concept of formation and perspectives of development Vladimir Isachenko, Friedrich Gagsteiger, Evgenia Isachenko and Juergen Weiss; 24. Transplantation of cryopreserved ovarian tissues Dror Meirow; 25. Whole ovary cryopreservation Jason G. Bromer and Pasquale Patrizio; 26. Transplantation of whole frozen-thawed ovaries Amir Arav and Yehudit Natan; 27. Ethical considerations in fertility cryopreservation in young cancer patients Edwin C. Hui; Index.