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Genome Editing and Engineering
From TALENs, ZFNs and CRISPRs to Molecular Surgery

CAD$195.95 (P)

Krishnarao Appasani, Raghu Kiran Appasani, Ben Farboud, Te-Wen Lo, Youjun Wu, Jiing-Kuan Yee, Andrew R. Bassett, Qianxin Wu, Quentin R. V. Ferry, Tudor Fulga, Jia Jia Chan, ZhiHao Kwok, Yvonne Tay, Yasuhiko Kato, Takashi Nakanishi, Hajime Watanabe, Ira L. Blitz, Chunxin Wang, Yingjie Wu, Martin F. Berthelsen, Martin K. Thomsen, Yonglun Luo, Bethany Redel, Chad O'Gorman, Randall S. Prather, Kristin M. Whitworth, Francesco Niola, Morten Frödin, Ella Paulina Thomson, Ruby Emily Moffat, James Nicholas Hughes, Paul Quinton Thomas, Eric P. Bennett, Ashley M. Jacobi, Garrett R. Rettig, Mark A. Behlke, Toshitsugu Fujita, Hodaka Fujii, Trinna L. Cuellar, Marinella Callow, Ted Lau, Colin Watanabe, Russell Bainer, Mike Costa, Benjamin Haley, Megan L. Ludwig, Nicole M. Michmerhuizen, Rebecca C. Hoesli, Jaqueline E. Mann, Samantha N. Devenport, Aditi S. Kulkarni, Andrew C. Birkeland, J. Chad Brenner, Michael Gapinske, Laurent Grant, Alexander Brown, Pablo Perez-Pinera, Shreevathsa S. Chilkunda, Vaishnavi Raghunathan, Suneel A. Narayanavari, Zoltán Ivics, Zsuzsanna Izsvák, Zhili Rong, Yang Xu, James L. Sherley, Rasmus Otkjaer Bak, Daniel Patrick Dever, Matthew H. Porteus, V. Lalgudi, Monte M. Winslow, Ian Paul Winters, Nalani Sachan, Madison Miller, Nitin H. Shirole, Serif Senturk, Vincenzo Corbo, Justin B. Kinney, Raffaella Sordella, Vinod Jaskula-Ranga, Stefan Haemmig, Mark W. Feinberg, Paige Charlins, Ramesh Akkina, Amanda Freire Assis, Cesar Speck Hernandez, Larissa Cotrim-Sousa, Rafaela Martins Felicio, Ernna Hérida Oliveira, Lorena Alves Teixeira, Valéria Valente, Wilson Araújo Silva, Jr, Geraldo A. Passos, Wendy J. M. Huang, Philip Webber, Rodney L. Sparks, Tetsuya Ishii, Sarah Chan
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  • Publication planned for: September 2018
  • availability: Not yet published - available from October 2018
  • format: Hardback
  • isbn: 9781107170377

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About the Authors
  • Recent advances in genome editing tools using endonucleases such as TALENs, ZFNs, and CRISPRs, combined with genomic engineering technologies, have opened up a wide range of opportunities from applications in the basic sciences and disease biology research, to the potential for clinical applications and the development of new diagnostic tools. This complete guide to endonuclease-based genomic engineering gives readers a thorough understanding of this rapidly expanding field. Chapters cover the discovery, basic science, and application of these techniques, focusing particularly on their potential relevance to the treatment of cancer, and cardiovascular and immunological disease. The final section discusses the legal and ethical issues which accompany the technology. Providing authoritative coverage of the potential that genome editing and engineering have, this is an ideal reference for researchers and graduate students and those working in the biotechnology and pharmaceutical industries, as well as in a clinical setting.

    • The first complete resource on the subject, including a dedicated section on the tools and technology platforms needed to make effective use of the techniques
    • The first volume to provide a bridge between the biology of endonucleases and technological developments and their implications in stem cell biology, synthetic biology and transcriptional regulation
    • Covers the applications of genome editing specifically in cancer, HIV, cardiovascular, retinal and infectious diseases
    • Discusses the legal and bioethical issues of the field, encouraging discussion about the application of these techniques in a clinical setting
    Read more

    Reviews & endorsements

    'This comprehensive book is welcome at this point of rapid growth in new technologies and discoveries such that keeping up with applications of genome engineering is challenging. Here we have both a general introduction to the field and to deep mechanisms and glimpses of future applications and ethical considerations - with gems for newcomers and experts alike.' George M. Church, Harvard University, Massachusetts and Massachusetts Institute of Technology

    'Krishnarao Appasani has assembled a comprehensive series of up-to-date chapters on endonuclease-based genomic engineering by experts and practitioners. The book presents a wide range of topics from basic science, applications to genetic screens and diseases, and legal and ethical issues. The many potential applications across a wide range of organisms are well illustrated. The book will become a must have for any researchers in academia and industry trying to keep up with this fast-moving field.' Norbert Perrimon, Harvard Medical School, Boston

    '… a remarkable book, which very successfully manages to provide deep insights into the various and latest aspects of gene editing and engineering. It is so far probably the best gene editing text collection available and highly suited to get a good understanding of the breadth and impact of this cutting-edge field of science.' Rodger Novak, President, Director and Chair of the Board CRISPR Therapeutics AG, Switzerland

    '… offers a widespread analysis of the state of the art concerning all the main tools available for modification of eukaryotic DNA in vivo, their application in metazoans and the societal issues involved. I thoroughly recommend it to anyone interested in gaining insight into this newest genetic revolution.' Francisco Juan Martinez Mojica, Universidad de Alicante, Spain

    'The field of precise genome engineering has been compiled into an excellent and essential guide through its landscape and it imparts state-of-the-art knowledge of its tools, its multiple research and clinical applications and related legal and ethical implications.' Shamim Herbert Rahman, Senior Research Scientist, Novo Nordisk A/S, Måløv, Denmark

    '… broadly covers recent development of genome editing from principle, history and usage to patent wars.' Keiji Nishida, Kobe University, Japan

    '… providing significant and useful information on current knowledge in the field and promoting the interaction between basic and pre-clinical/clinical research … of potential interest to a broad readership interested in basic and translational research.' Antonio Russo, 'P. Giaccone' University Hospital, University of Palermo, Italy

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    Product details

    • Publication planned for: September 2018
    • format: Hardback
    • isbn: 9781107170377
    • length: 498 pages
    • dimensions: 252 x 177 x 30 mm
    • weight: 1.19kg
    • contains: 122 b/w illus. 32 colour illus. 9 tables
    • availability: Not yet published - available from October 2018
  • Table of Contents

    List of contributors
    Forewords
    Preface
    List of abbreviations
    Part I. Biology of Endonucleases (Zinc-Finger Nuclease, TALENs and CRISPRs) and Regulatory Networks:
    1. Introduction to genome editing and engineering: from Talens, ZFNs and CRISPRs to molecular surgery
    2. Targeted genome editing techniques in C. elegans and other nematode species
    3. Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
    4. In vivo studies of miRNA target interactions using site-specific genome engineering
    5. Don't kill the messenger: employing genome editing to study regulatory RNA interactions
    Part II. Genome Editing in Model Organisms:
    6. Genome editing in the crustacean Daphnia magna using CRISPR/Cas and TALEN systems
    7. Leapfrogging: a method for targeting genome editing to the germline
    8. Genome editing with desired mutations (knock-in) by CRISPR in model organisms
    9. Genetically engineered pig models for human diseases using ZFNs, TALENs and CRISPR-Cas9
    10. Gene editing to create agricultural and biomedical swine models
    11. Generation of new model cell lines using ssODN knock-in donors and FACS-based genome editing
    Part III. Technology Development and Screening:
    12. CRISPR genome editing in mice
    13. Detection of insertion/deletion (indel) events after genome targeting: pros and cons of the available methods
    14. Application of TAL proteins and the CRISPR system to purification of specific genomic regions for locus-specific identification of chromatin-associated molecules
    15. Application of CRISPR for pooled, vector-based functional genomic screening in mammalian cell lines
    16. Generation and utilization of CRISPR/Cas9 screening libraries in mammalian cells
    Part IV. Genome Editing in Stem Cells and Regenerative Biology:
    17. Targeted genome editing using nuclease assisted vector integration
    18. Genome engineering using Sleeping Beauty transposition in vertebrates
    19. Genome editing of pluripotent stem cells
    20. Principles for targeting adult tissue stem cells to achieve durable gene and gene-editing therapies
    21. Therapeutic genome editing in human hematopoietic stem and progenitor cells
    Part V. Genome Editing in Disease Biology:
    22. CRISPR/Cas9-Based in vivo models of cancer
    23. Inducible CRISPR-based genome editing for the characterization of cancer genes
    24. Genome editing for retinal diseases
    25. Manipulation of long non-coding RNAs in cardiovascular disease using genome-editing technology
    26. Gene silencing, disruption and latency reactivation with RNA-based and gene editing CRISPR/Cas, ZFN and TALEN technologies for HIV-1/AIDS therapies
    27. Use of the CRISPR-Cas9 system for genome editing of immune system cells, defense against HIV-1 and cancer therapies
    28. Harnessing therapeutic potential of long non-coding RNAs in immunity
    Part VI. Legal (Intelluctual Property) and Bioethical Issues of Genome Editing:
    29. Patening of CRISPR-based inventions
    30. Patent, ownership, and licensing issues of CRISPR-based genome editing: impact on universities and their licensees
    31. The ethics of human genome editing
    32. Regulating human genome editing: negotiating ethical concerns through science and policy
    Index.

  • Editor

    Krishnarao Appasani, GeneExpression Systems, Inc.
    Krishnarao Appasani is the Founder and Chief Executive Officer of GeneExpression Systems. He is an award winning scientist, and has edited several books, including: Optogenetics: From neuronal function to mapping and disease biology (Cambridge, 2017); Genome-Wide Association Studies: From Polymorphism to Personalized Medicine (Cambridge, 2016); Epigenomics: From Chromatin Biology to Therapeutics (Cambridge, 2012); MicroRNAs: From Basic Science to Disease Biology (Cambridge, 2007); and RNA Interference: From Basic Science to Drug Development (Cambridge, 2005).

    Foreword

    George M. Church

    Contributors

    Krishnarao Appasani, Raghu Kiran Appasani, Ben Farboud, Te-Wen Lo, Youjun Wu, Jiing-Kuan Yee, Andrew R. Bassett, Qianxin Wu, Quentin R. V. Ferry, Tudor Fulga, Jia Jia Chan, ZhiHao Kwok, Yvonne Tay, Yasuhiko Kato, Takashi Nakanishi, Hajime Watanabe, Ira L. Blitz, Chunxin Wang, Yingjie Wu, Martin F. Berthelsen, Martin K. Thomsen, Yonglun Luo, Bethany Redel, Chad O'Gorman, Randall S. Prather, Kristin M. Whitworth, Francesco Niola, Morten Frödin, Ella Paulina Thomson, Ruby Emily Moffat, James Nicholas Hughes, Paul Quinton Thomas, Eric P. Bennett, Ashley M. Jacobi, Garrett R. Rettig, Mark A. Behlke, Toshitsugu Fujita, Hodaka Fujii, Trinna L. Cuellar, Marinella Callow, Ted Lau, Colin Watanabe, Russell Bainer, Mike Costa, Benjamin Haley, Megan L. Ludwig, Nicole M. Michmerhuizen, Rebecca C. Hoesli, Jaqueline E. Mann, Samantha N. Devenport, Aditi S. Kulkarni, Andrew C. Birkeland, J. Chad Brenner, Michael Gapinske, Laurent Grant, Alexander Brown, Pablo Perez-Pinera, Shreevathsa S. Chilkunda, Vaishnavi Raghunathan, Suneel A. Narayanavari, Zoltán Ivics, Zsuzsanna Izsvák, Zhili Rong, Yang Xu, James L. Sherley, Rasmus Otkjaer Bak, Daniel Patrick Dever, Matthew H. Porteus, V. Lalgudi, Monte M. Winslow, Ian Paul Winters, Nalani Sachan, Madison Miller, Nitin H. Shirole, Serif Senturk, Vincenzo Corbo, Justin B. Kinney, Raffaella Sordella, Vinod Jaskula-Ranga, Stefan Haemmig, Mark W. Feinberg, Paige Charlins, Ramesh Akkina, Amanda Freire Assis, Cesar Speck Hernandez, Larissa Cotrim-Sousa, Rafaela Martins Felicio, Ernna Hérida Oliveira, Lorena Alves Teixeira, Valéria Valente, Wilson Araújo Silva, Jr, Geraldo A. Passos, Wendy J. M. Huang, Philip Webber, Rodney L. Sparks, Tetsuya Ishii, Sarah Chan

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