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Commercialization of plant-based vaccines from research and development to manufacturing

  • John A. Howard (a1)

The benefits of using plant-based oral vaccines are discussed. Transgenic maize expressing an antigen of transmissible gastroenteritis virus (TGEV) is reported as a model to demonstrate efficacy. Young pigs that were fed the TGEV corn orally were protected against challenge with virulent TGEV. Additional parameters important in providing a reliable and consistent supply of plant-based vaccines are discussed. Finally, vaccines developed in maize are evaluated for their potential to contaminate either the food supply or the environment.

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Hood, EE, Witcher, DR, Maddock, S, Meyer, T, Baszczynski, C, Bailey, M, Flynn, P, Register, J, Marshall, L, Bond, D, Kulisek, E, Kusnadi, A, Evangelista, R, Nikolov, Z, Wooge, C, Mehigh, RJ, Herman, R, Kappel, WK, Ritland, D, Li, CP and Howard, JA (1997). Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Molecular Breeding 3: 291306.
Jilka, JM, Hood, EE, Dose, R and Howard, J (1999). The benefits of proteins produced in transgenic plants. [Review]. AgBiotechNet 1: ABN 027.
Kusnadi, AR, Nikolov, ZL and Howard, JA (1997). Production of recombinant proteins in transgenic plants: practical considerations. Biotechnology and Bioengineering 56: 473484.
Lamphear, BJ, Streatfield, SJ, Jilka, JM, Brooks Barker, DK, Turner, DD, Delaney, E, Garcia, M, Wiggins, B, Woodard, SL, Hood, EE, Tizard, IR, Lawhorn, B and Howard, JA (2002). Delivery of subunit vaccines in maize seed. Journal of Controlled Release 85: 169180.
Laude, H, Rasschaert, D, Delmas, B, Godet, M, Gelfi, J and Charley, B (1990). Molecular biology of transmissible gastroenteritis virus. Veterinary Microbiology 23: 147154.
Ma, JK-C, Drake, PMW and Christou, P (2003). The production of recombinant pharmaceutical proteins in plants. Nature Reviews, Genetics 4: 794805.
Streatfield, SJ and Howard, JA (2003). Plant-based vaccines. International Journal for Parasitology 33: 479493.
Streatfield, SJ, Jilka, JM, Hood, EE, Turner, DD, Bailey, MR, Mayor, JM, Woodard, SL, Beifuss, KK, Horn, ME, Delaney, DE, Tizard, IR and Howard, JA (2001). Plant-based vaccines: unique advantages. Vaccine 19: 27422748.
Streatfield, SJ, Mayor, JM, Barker, DK, Brooks, C, Lamphear, BJ, Woodard, SL, Beifuss, KK, Vicuna, DV, Massey, LA, Horn, ME, Delaney, DE, Nikolov, ZL, Hood, EE, Jilka, JM and Howard, JA (2002). 2000 Congress Symposium on Molecular Farming: Development of an edible subunit vaccine in corn against enterotoxigenic strains of Escherichia coli. In Vitro Cellular Developmental Biology Plant 38: 1117.
Streatfield, S, Lane, JR, Brooks, CA, Barker, DK, Poage, ML, Mayor, JM, Lamphear, BJ, Drees, CF, Jilka, JM, Hood, EE and Howard, JA (2003). Corn as a production system for human and animal vaccines. Vaccine 21: 812815.
Witcher, DR, Hood, EE, Peterson, D, Bailey, M, Bond, D, Kusnadi, A, Evangelista, R, Nikolov, Z, Wooge, C, Mehigh, R, Kappel, W, Register, J and Howard, JA (1998). Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants. Molecular Breeding 4: 301312.
Woodard, SL, Mayor, JM, Bailey, MR, Barker, DK, Love, RT, Lane, JR, Delaney, DE, McComas-Wagner, JM, Mallubhotla, HD, Hood, EE, Gangott, LJ, Tichy, SE and Howard, JA (2003). Maize (Zea mays) -derived bovine trypsin: characterization of the first large-scale, commercial product from transgenic plants. Biotechnology and Applied Biochemistry 38: 123130.
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Animal Health Research Reviews
  • ISSN: 1466-2523
  • EISSN: 1475-2654
  • URL: /core/journals/animal-health-research-reviews
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