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A review of porcine tonsils in immunity and disease

  • Dennis C. Horter (a1), Kyoung-Jin Yoon (a1) and Jeffrey J. Zimmerman (a1)

The porcine tonsils are a group of lymphoepithelial tissues located at the common openings of the gastrointestinal and respiratory tracts. The tonsils participate in a variety of functions involving innate, cellular and humoral immunity at the local and systemic levels. Among these immunological functions is the continuous surveillance for the presence of foreign antigens at the openings of the gastrointestinal and respiratory tracts. Within the pig, the movement of lymphocytes, cytokines and chemotactic molecules from the tonsils to other lymphoid organs confers immunity to other portals of pathogen entry and facilitates an efficient and rapid systemic immune response. In spite of the immunological nature of the tonsils, some microorganisms have acquired adaptations that allow them to circumvent the tonsillar immune defenses and utilize the tonsils as a site of entry, replication and colonization. Several bacterial and viral pathogens persist asymptomatically within the tonsils, making identification of asymptomatic carrier animals difficult in disease control and/or pathogen elimination. This paper reviews the current information on the anatomy, immunology and pathobiology of porcine tonsils and discusses the tonsils as a site of pathogen entry, replication and colonization using Salmonella spp., classical swine fever virus and porcine reproductive and respiratory syndrome virus as examples.

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E Albina , F Madec , R Cariolet and J Torrison (1994). Immune response and persistence of the porcine reproductive and respiratory syndrome virus in infected pigs and farm units. Veterinary Record 134: 567573.

S Alexandersen , MB Oleksiewicz and AI Donaldson (2001). The early pathogenesis of foot-and-mouth disease in pigs infected by contact: a quantitative time-course study using TaqMan RT–PCR. Journal of General Virology 82: 747755.

R Allende , WW Laegreid , GF Kutish , JA Galeota , RW Wills and FA Osorio (2000). Porcine reproductive and respiratory syndrome virus: description of persistence in individual pigs upon experimental infection. Journal of Virology 74: 1083410837.

M Baele , K Chiers , LA Devriese , HE Smith , HJ Wisselink , M Vaneechoutte and F Haesebrouck (2001). The gram-positive tonsillar and nasal flora of piglets before and after weaning. Journal of Applied Microbiology 91: 9971003.

GC Bagby Jr, VD Rigas , RM Bennett , AA Vandenbark and HS Garewal (1981). Interaction of lactoferrin, monocytes, and T lymphocyte subsets in the regulation of steady-state granulopoiesis in vitro. Journal of Clinical Investigation 68: 5663.

A Baskerville and C Dow (1973). Pathology of experimental pneumonia in pigs produced by Salmonella choleraesuis. Journal of Comparative Pathology 83: 207215.

EM Bautista and TW Molitor (1997). Cell-mediated immunity to porcine reproductive and respiratory syndrome virus in swine. Viral Immunology 10: 8394.

GT Belz (1998). Intercellular and lymphatic pathways associated with tonsils of the soft palate in young pigs. Anatomy and Embryology 197: 331340.

GT Belz and TJ Heath (1996). Tonsils of the soft palate of young pigs: crypt structure and lymphoepithelium. Anatomical Record 245: 102113.

J Beyer , D Fichtner , H Schirrmeier , U Polster , E Weiland and H Wege (2000). Porcine reproductive and respiratory syndrome virus (PRRSV): kinetics of infection in lymphatic organs and lung. Journal of Veterinary Medicine B, Infectious Diseases And Veterinary Public Health 47: 925.

AT Bianchi , RJ Zwart , SH Jeurissen and HW Moonen-Leusen (1992). Development of the B- and T-cell compartments in porcine lymphoid organs from birth to adult life: an immunohistological approach. Veterinary Immunology and Immunopathology 33: 201221.

M Boeker , R Pabst and J Rothkotter (1999). Quantification of B, T and null lymphocyte subpopulations in the blood and lymphoid organs of the pig. Immunobiology 201: 7487.

P Brandtzaeg , ES Baekkefold , IN Farstad , FL Jahnsen , FE Johanson , EM Nilsen and T Yamanaka (1999). Regional specialization in the mucosal immune system: what happens in the microcompartments? Immunology Today 20: 141151.

C Choi and C Chae (2003). Detection of classical swine fever virus in boar semen by reverse transcription–polymerase chain reaction. Journal of Veterinary Diagnostic Investigations 15: 3541.

NR Cooper (1985). The classical complement pathway. Activation and regulation of the first complement component. Advances in Immunology 37: 151216.

L Darwich , J Segales , M Domingo and E Mateu (2002). Changes in CD4(+), CD8(+), CD4(+), CD8(+), and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and healthy pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues. Clinical and Diagnostic Laboratory Immunology 9: 236–42.

PR Davies and CJ Ossowicz (1991). Evaluation of methods used for detecting Streptococcus suis type 2 in tonsils, and investigation of the carrier state in pigs. Research in Veterinary Science 50: 190194.

A Dekker , P Moonen , EA de Boer-Luijtze and C Terpstra (1995). Pathogenesis of swine vesicular disease after exposure of pigs to an infected environment. Veterinary Microbiology 45: 243–50.

LA Devriese , J Hommez , B Pot and F Haesebrouck (1994). Identification and composition of the streptococcal and enterococcal flora of tonsils, intestines and faeces of pigs. Journal of Applied Bacteriology 77: 3136.

MJ Dillender and JK Lunney (1993). Characteristics of T lymphocyte cell lines established from NIH minipigs challenge inoculated with Trichinella spiralis. Veterinary Immunology and Immunopathology 35: 301319.

S Edwards , A Fukusho , P-C Lefevre , A Lipowski , Z Pejsak , P Roehe and J Westergaard (2000). Classical swine fever: The global situation. Veterinary Microbiology 73: 103119.

PJ Fedorka-Cray , SC Whipp , RE Isaacson , N Nord and K Lager (1994). Transmission of Salmonella typhimurium to swine. Veterinary Microbiology 41: 333344.

A Fleming (1922). On a remarkable bacteriolytic element found in tissues and secretions. Proceedings of the Royal Society of London Series B 93: 306317.

T Gram , P Ahrens and JP Nielsen (1996). Evaluation of a PCR for detection of Actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. Veterinary Microbiology 51: 95104.

JT Gray , PJ Fedorka-Cray , TJ Stabel and MR Ackermann (1995). Influence of inoculation route on the carrier state of Salmonella choleraesuis in swine. Veterinary Microbiology 47: 4359.

RE Hancock and MG Scott (2000). The role of antimicrobial peptides in animal defenses. Proceedings of the National Academy of Sciences of the United States of America 97: 88568861.

PA Harms , SD Sorden , PG Halbur , SR Bolin , KM Lager , I Morozov and PS Paul (2001). Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Veterinary Pathology 38: 528539.

DC Horter , RM Pogranichniy , CC Chang , RB Evans , KJ Yoon and JJ Zimmerman (2002). Characterization of the carrier state in porcine reproductive and respiratory syndrome virus infection. Veterinary Microbiology 86: 213–28.

EW Howerth , DE Stallknecht , M Dorminy , T Pisell and GR Clarke (1997). Experimental vesicular stomatitis in swine: effects of route of inoculation and steroid treatment. Journal of Veterinary Diagnostic Investigations 9: 136142.

AJ Howie (1981). The cells in tonsillar crypts. Clinical Otolaryngology and Allied Sciences 7: 3544.

D Kassay and A Sandor (1962). The crypt system of the palatine tonsils. Archives of Otolaryngology 75: 144155.

SV Kotenko , S Saccani , LS Izotova , OV Mirochnitchenko and S Pestka (2000). Human cytomegalovirus harbors its own unique IL-10 homologue (cmvIL-10). Proceedings of the National Academy of Sciences of the United States of America 97: 16951700.

A Krmpoti , DH Busch , I Bubi , F Gebhardt , H Hengel , M Hasan , AA Scalzo , UH Koszinowski and S Jonji (2002). MCMV glycoprotein gp40 confers virus resistance to CD8+ T cells and NK cells in vivo. Nature Immunology 3: 529535.

K Kume , T Nakai and A Sawata (1984). Isolation of Haemophilus pleuropneumoniae from the nasal cavities of healthy pigs. Japanese Journal of Veterinary Science 46: 641647.

J Laufer , R Oren , I Goldberg , A Horwitz , J Kopolovic , Y Chowers and JH Passwell (2000). Cellular localization of complement C3 and C4 transcripts in intestinal specimens from patients with Crohn's disease. Clinical Experimental Immunology 120: 3037.

J Levitskaya , M Coram , V Levitsky , S Imreh , PM Steigerwald-Mullen , G Klein , MG Kurilla and MG Masucci (1995). Inhibition of antigen processing by the internal repeat region of the Epstein–Barr virus nuclear antigen 1. Nature 375: 685688.

J Levitskaya , A Sharipo , A Leonchiks , A Ciechanover and MG Masucci (1997). Inhibition of ubiquitin/proteasome-dependent protein degradation by the Gly-Ala repeat domain of the Epstein–Barr virus nuclear antigen 1. Proceedings of the National Academy of Sciences of the United States of America 94: 1261612621.

HD Loemba , S Mounir , H Mardassi , D Archambault and S Dea (1996). Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus. Archives of Virology 141: 751761.

LW Madsen , B Svensmark , K Elvestad , B Aalbaek and HE Jensen (2002). Streptococcus suis serotype 2 infection in pigs: new diagnostic and pathogenetic aspects. Journal of Comparative Pathology 126: 5765.

DK Meyerholz , TJ Stabel , MR Ackermann , SA Carlson , BD Jones and J Pohlenz (2002). Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum. Veterinary Pathology 39: 712720.

S Murakami , R Azuma , T Koeda , H Oomi , T Watanabe and H Fujiwara (1998). Immunohistochemical detection for Actinomyces sp. in swine tonsillar abscess and granulomatous mastitis. Mycopathologia 141: 15–9.

MR Neutra , E Pringault and JP Kraehenbuhl (1996). Antigen sampling across epithelial barriers and induction of mucosal immune responses. Annual Review of Immunology 14: 275300.

B Nielsen , C Heisel and A Wingstrand (1996). Time course of the serological response to Yersinia enterocolitica O:3 in experimentally infected pigs. Veterinary Microbiology 48: 293303.

R Pabst and RM Binns (1989). Heterogeneity of lymphocyte homing physiology: several mechanisms operate in the control of migration to lymphoid and non-lymphoid organs in vivo. Immunology Reviews 108: 83109.

R Pabst and E Nowara (1984). The emigration of lymphocytes from palatine tonsils after local labeling. Archives of Otorhinolaryngology 240: 713.

G Pantaleo , C Graziosi , L Butini , PA Pizzo , SM Schnittman , DP Kotler and AS Fauci (1991). Lymphoid organs function as major reservoirs for human immunodeficiency virus. Proceedings of the National Academy of Sciences of the United States of America 88: 98389842.

JM Payne and JB Derbyshire (1963). Portals of entry for bacterial infection in calves and piglets with particular reference to the tonsil. Journal of Pathology and Bacteriology 85: 171178.

S Payne , B Parekh , RC Montelaro and CJ Issel (1984). Genomic alterations associated with persistent infections by equine infectious anaemia virus, a retrovirus. Journal of General Virology 65: 13951399.

MD Pescovitz , AG Sakopoulos , JA Gaddy , RJ Husmann and FA Zuckermann (1994). Porcine peripheral blood CD4+/CD8+ dual expressing T-cells. Veterinary Immunology and Immunopathology 43: 53.

HL Ploegh (1998). Viral strategies of immune evasion. Science 280: 248–53.

W Rabsch , H Tschape and AJ Baumler (2001). Non-typhoidal salmonellosis: emerging problems. Microbes and Infection 3: 237247.

AA Ressang (1973). Studies on the pathogenesis of hog cholera. I. Demonstration of hog cholera virus subsequent to oral exposure. Zentralblatt für Veterinärmedizin 20: 256271.

KD Rossow , DA Benfield , SM Goyal , EA Nelson , J Christopher-Hennings and JE Collins (1996). Chronological immunohistochemical detection and localization of porcine reproductive and respiratory syndrome virus in gnotobiotic pigs. Veterinary Pathology 33: 551556.

A Saalmuller , MJ Redehasse , HJ Buhring , S Jonjic and UH Koszinowski (1987). Simultaneous expression of CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes. European Journal of Immunology 17: 1297.

KS Sahasrabudhe , JR Kimball , TH Morton , A Weinberg and BA Dale (2000). Expression of the antimicrobial peptide, human beta-defensin 1, in duct cells of minor salivary glands and detection in saliva. Journal of Dental Research 79: 16691677.

MW Salles and DM Middleton (2000). Lymphocyte subsets in porcine tonsillar crypt epithelium. Veterinary Immunology and Immunopathology 77: 133144.

MW Salles , J Perez-Casal , P Willson and DM Middleton (2002). Changes in the leucocyte subpopulations of the palatine tonsillar crypt epithelium of pigs in response to Streptococcus suis type 2 infection. Veterinary Immunology and Immunopathology 87: 5163.

F Sallusto , A Lanzavecchia and CR Mackay (1998). Chemokines and chemokine receptors in T-cell priming and Th1/Th2-mediated responses. Immunology Today 19: 568574.

Y Sato , K Wake and I Watanabe (1990). Differentiation of crypt epithelium in human palatine tonsils: the microenvironment of crypt epithelium as a lymphoepithelial organ. Archives of Histology and Cytology 53: 4154.

TG Senkevich , JJ Bugert , JR Sisler , EV Koonin , G Darai and B Moss (1996). Genome sequence of a human tumorigenic poxvirus: prediction of specific host-response-evasion genes. Science 273: 813816.

A Sharipo , M Imreh , A Leonchiks , C-I Brändén and MG Masucci (2001). cis-Inhibition of proteasomal degradation by viral repeats: impact of length and amino acid composition. FEBS Letters 499: 137142.

AH Stephano , GM Gay and TC Ramirez (1988). Encephalomyelitis, reproductive failure and corneal opacity in pigs, associated with a new paramyxovirus infection (blue eye). Veterinary Record 122: 610.

C Terpstra and AJ de Smit (2000). The 1997/1998 epizootic of swine fever in the Netherlands: control strategies under a non-vaccination regimen. Veterinary Microbiology 77: 315.

C Terpstra , G Wensvoort and JMA Pol (1991). Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch's postulates fulfilled. Veterinary Quarterly 13: 131136.

S Terzic , L Sver , I Valpotic , M Lojkic , Z Miletic , L Jemersic , G Lackovic , A Kovsca-Janjatovic and N Orsolic (2002). Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs. Veterinary Research Communications 26: 273283.

K Uchiya , MA Barbieri , K Funato , AH Shah , PD Stahl and EA Groisman (1999). A Salmonella virulence protein that inhibits cellular trafficking. EMBO Journal 18: 39243933.

JT Van Oirschot and C Terpstra (1977). A congenital persistent swine fever infection. I. Clinical and virological observations. Veterinary Microbiology 2: 121132.

M Velinova , C Thielen , F Melot , J Donga , S Eicher , E Heinen and N Antoine (2001). New histochemical and ultrastructural observations on normal bovine tonsils. Veterinary Record 149: 613617.

G Wensvoort , C Terpstra , JM Pol , EA ter Laak , M Bloemraad , EP de Kluyver , C Kragten , L van Buiten , A den Besten and F Wagenaar (1991). Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly 13: 121130.

RW Wills , JJ Zimmerman , KJ Yoon , SL Swenson , MJ McGinley , HT Hill , KB Platt , J Christopher-Hennings and EA Nelson (1997). Porcine reproductive and respiratory syndrome virus: a persistent infection. Veterinary Microbiology 55: 231240.

MT Winkler , A Doster and C Jones (2000). Persistence and reactivation of bovine herpesvirus 1 in the tonsils of latently infected calves. Journal of Virology 74: 53375346.

G Zhang , CR Ross and F Blecha (2000). Porcine antimicrobial peptides: new prospects for ancient molecules of host defense. Veterinary Research 31: 277296.

FA Zuckermann (1999). Extrathymic CD4/CD8 double positive T cells. Veterinary Immunology and Immunopathology 72: 5566.

FA Zuckermann and HR Gaskins (1996). Distribution of porcine CD4/CD8 double-positive T lymphocytes in mucosa-associated lymphoid tissues. Immunology 87: 493499.

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Animal Health Research Reviews
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