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Swine influenza vaccines: current status and future perspectives

  • Wenjun Ma (a1) and Jürgen A. Richt (a1)

Swine influenza is an important contagious disease in pigs caused by influenza A viruses. Although only three subtypes of influenza A viruses, H1N1, H1N2 and H3N2, predominantly infect pigs worldwide, it is still a big challenge for vaccine manufacturers to produce efficacious vaccines for the prevention and control of swine influenza. Swine influenza viruses not only cause significant economic losses for the swine industry, but are also important zoonotic pathogens. Vaccination is still one of the most important and effective strategies to prevent and control influenza for both the animal and human population. In this review, we will discuss the current status of swine influenza worldwide as well as current and future options to control this economically important swine disease.

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AF Antonis , CJ Bruschke , P Rueda , L Maranga , JI Casal , C Vela , LA Hilgers , PB Belt , K Weerdmeester , MJ Carrondo and JP Langeveld (2006). A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure. Vaccine 24: 54815490.

RB Belshe (2004). Current status of live attenuated influenza virus vaccine in the US. Virus Research 103: 177185.

JR Bennink , JW Yewdell and W Gerhard (1982). A viral polymerase involved in recognition of influenza virus-infected cells by a cytotoxic T-cell clone. Nature 296: 7576.

P Berglund , MN Fleeton , C Smerdou and P Liljestrom (1999). Immunization with recombinant Semliki Forest virus induces protection against influenza challenge in mice. Vaccine 17: 497507.

H Bisht , A Roberts , L Vogel , A Bukreyev , PL Collins , BR Murphy , K Subbarao and B Moss (2004). Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice. Proceedings of the National Academy of Sciences USA 101: 66416646.

CC Breathnach , HJ Clark , RC Clark , CW Olsen , HG Townsend and DP Lunn (2006). Immunization with recombinant modified vaccinia Ankara (rMVA) constructs encoding the HA or NP gene protects ponies from equine influenza virus challenge. Vaccine 24: 11801190.

RA Bright , DM Carter , S Daniluk , FR Toapanta , A Ahmad , V Gavrilov , M Massare , P Pushko , N Mytle , T Rowe , G Smith and TM Ross (2007). Influenza virus-like particles elicit broader immune responses than whole virion inactivated influenza virus or recombinant hemagglutinin. Vaccine 25: 38713878.

RA Bright , DM Carter , CJ Crevar , FR Toapanta , JD Steckbeck , KS Cole , NM Kumar , P Pushko , G Smith , TM Tumpey and TM Ross (2008). Cross-clade protective immune responses to influenza viruses with H5N1 HA and NA elicited by an influenza virus-like particle. PLoS One 3: e1501.

IH Brown (2000). The epidemiology and evolution of influenza viruses in pigs. Veterinary Microbiology 74: 2946.

IH Brown (2008). The role of pigs in interspecies transmission. In: HD Klenk , MN Matrosovich and J Stech (eds) Avian Influenza. Basel, Karger: Monogr Virol, pp. 88100.

IH Brown , S Ludwig , CW Olsen , C Hannoun , C Scholtissek , VS Hinshaw , PA Harris , JW McCauley , I Strong and DJ Alexander (1997). Antigenic and genetic analyses of H1N1 influenza A viruses from European pigs. Journal of General Virology 78: 553562.

IH Brown , PA Harris , JW McCauley and DJ Alexander (1998). Multiple genetic reassortment of avian and human influenza A viruses in European pigs, resulting in the emergence of an H1N2 virus of novel genotype. Journal of General Virology 79: 29472955.

F Carrat and A Flahault (2007). Influenza vaccine: the challenge of antigenic drift. Vaccine 25: 68526862.

MR Castrucci , I Donatelli , L Sidoli , G Barigazzi , Y Kawaoka and RG Webster (1993). Genetic reassortment between avian and human influenza A viruses in Italian pigs. Virology 193: 503506.

TM Chambers , VS Hinshaw , Y Kawaoka , BC Easterday and RG Webster (1991). Influenza viral infection of swine in the United States 1988–1989. Archives of Virology 116: 261265.

TM Chambers , RE Holland , LR Tudor , HG Townsend , A Cook , J Bogdan , DP Lunn , S Hussey , P Whitaker-Dowling , JS Youngner , RW Sebring , SJ Penner and GL Stiegler (2001). A new modified live equine influenza virus vaccine: phenotypic stability, restricted spread and efficacy against heterologous virus challenge. Equine Veterinary Journal 33: 630636.

CY Chang , CC Huang , TS Huang , MC Deng , MH Jong and FI Wang (2006). Isolation and characterization of a Sagiyama virus from domestic pigs. Journal of Veterinary Diagnostic Investigation 18: 156161.

AN Chanturiya , G Basanez , U Schubert , P Henklein , JW Yewdell and J Zimmerberg (2004). PB1-F2, an influenza A virus-encoded proapoptotic mitochondrial protein, creates variably sized pores in planar lipid membranes. Journal of virology 78: 63046312.

MW Chen , TJ Cheng , Y Huang , JT Jan , SH Ma , AL Yu , CH Wong and DD Ho (2008). A consensus-hemagglutinin-based DNA vaccine that protects mice against divergent H5N1 influenza viruses. Proceedings of the National Academy of Sciences USA 105: 1353813543.

Z Chen , K Matsuo , H Asanuma , H Takahashi , T Iwasaki , Y Suzuki , C Aizawa , T Kurata and S Tamura (1999a). Enhanced protection against a lethal influenza virus challenge by immunization with both hemagglutinin- and neuraminidase-expressing DNAs. Vaccine 17: 653659.

Z Chen , T Yoshikawa , S Kadowaki , Y Hagiwara , K Matsuo , H Asanuma , C Aizawa , T Kurata and S Tamura (1999b). Protection and antibody responses in different strains of mouse immunized with plasmid DNAs encoding influenza virus haemagglutinin, neuraminidase and nucleoprotein. Journal of General Virology 80: 25592564.

A Cosma , R Nagaraj , S Buhler , J Hinkula , DH Busch , G Sutter , FD Goebel and V Erfle (2003). Therapeutic vaccination with MVA-HIV-1 nef elicits Nef-specific T-helper cell responses in chronically HIV-1 infected individuals. Vaccine 22: 2129.

A Cosma , R Nagaraj , C Staib , C Diemer , F Wopfner , H Schatzl , DH Busch , G Sutter , FD Goebel and V Erfle (2007). Evaluation of modified vaccinia virus Ankara as an alternative vaccine against smallpox in chronically HIV type 1-infected individuals undergoing HAART. AIDS Research and Human Retroviruses 23: 782793.

MM Cox and JR Hollister (2009). FluBlok, a next generation influenza vaccine manufactured in insect cells. Biologicals 37: 182189.

NJ Cox and K Subbarao (1999). Influenza. Lancet 354: 12771282.

RJ Cox , KA Brokstad and P Ogra (2004). Influenza virus: immunity and vaccination strategies. Comparison of the immune response to inactivated and live, attenuated influenza vaccines. Scandinavian Journal of Immunology 59: 115.

J Crawford , B Wilkinson , A Vosnesensky , G Smith , M Garcia , H Stone and ML Perdue (1999). Baculovirus-derived hemagglutinin vaccines protect against lethal influenza infections by avian H5 and H7 subtypes. Vaccine 17: 22652274.

RJ Drape , MD Macklin , LJ Barr , S Jones , JR Haynes and HJ Dean (2006). Epidermal DNA vaccine for influenza is immunogenic in humans. Vaccine 24: 44754481.

I Drexler , C Staib and G Sutter (2004). Modified vaccinia virus Ankara as antigen delivery system: how can we best use its potential? Current Opinion in Biotechnology 15: 506512.

EJ Dunham , VG Dugan , EK Kaser , SE Perkins , IH Brown , EC Holmes and JK Taubenberger (2009). Different evolutionary trajectories of European avian-like and classical swine H1N1 influenza A viruses. Journal of Virology 83: 54855494.

GA Elmowalid , M Qiao , SH Jeong , BB Borg , TF Baumert , RK Sapp , Z Hu , K Murthy and TJ Liang (2007). Immunization with hepatitis C virus-like particles results in control of hepatitis C virus infection in chimpanzees. Proceedings of the National Academy of Sciences USA 104: 84278432.

SL Epstein , A Stack , JA Misplon , CY Lo , H Mostowski , J Bennink and K Subbarao (2000). Vaccination with DNA encoding internal proteins of influenza virus does not require CD8(+) cytotoxic T lymphocytes: either CD4(+) or CD8(+) T cells can promote survival and recovery after challenge. International Immunology 12: 91101.

MM Erdman , KI Kamrud , DL Harris and J Smith (2010). Alphavirus replicon particle vaccines developed for use in humans induce high levels of antibodies to influenza virus hemagglutinin in swine: proof of concept. Vaccine 28: 594596.

E Eriksson , F Yao , T Svensjo , T Winkler , J Slama , MD Macklin , C Andree , M McGregor , V Hinshaw and WF Swain (1998). In vivo gene transfer to skin and wound by microseeding. Journal of Surgical Research 78: 8591.

EF Fynan , HL Robinson and RG Webster (1993). Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine. DNA Cell Biology 12: 785789.

A Gambotto , SM Barratt-Boyes , MD de Jong , G Neumann and Y Kawaoka (2008). Human infection with highly pathogenic H5N1 influenza virus. Lancet 371: 14641475.

A Gamvrellis , D Leong , JC Hanley , SD Xiang , P Mottram and M Plebanski (2004). Vaccines that facilitate antigen entry into dendritic cells. Immunology and Cell Biology 82: 506516.

A Garcia-Sastre , A Egorov , D Matassov , S Brandt , DE Levy , JE Durbin , P Palese and T Muster (1998). Influenza A virus lacking the NS1 gene replicates in interferon-deficient systems. Virology 252: 324330.

NP Goonetilleke , H McShane , CM Hannan , RJ Anderson , RH Brookes and AV Hill (2003). Enhanced immunogenicity and protective efficacy against Mycobacterium tuberculosis of bacille Calmette-Guerin vaccine using mucosal administration and boosting with a recombinant modified vaccinia virus Ankara. Journal of Immunology 171: 16021609.

F Gotch , A McMichael , G Smith and B Moss (1987). Identification of viral molecules recognized by influenza-specific human cytotoxic T lymphocytes. Journal of Experimental Medicine 165: 408416.

JR Haynes (2009). Influenza virus-like particle vaccines. Expert Review of Vaccines 8: 435445.

JR Haynes , L Dokken , JA Wiley , AG Cawthon , J Bigger , AG Harmsen and C Richardson (2009). Influenza-pseudotyped Gag virus-like particle vaccines provide broad protection against highly pathogenic avian influenza challenge. Vaccine 27: 530541.

F He , S Madhan and J Kwang (2009). Baculovirus vector as a delivery vehicle for influenza vaccines. Expert Review of Vaccines 8: 455467.

PP Heinen , EA de Boer-Luijtze and AT Bianchi (2001). Respiratory and systemic humoral and cellular immune responses of pigs to a heterosubtypic influenza A virus infection. Journal of General Virology 82: 26972707.

VS Hinshaw , WJ Bean Jr, RG Webster and BC Easterday (1978). The prevalence of influenza viruses in swine and the antigenic and genetic relatedness of influenza viruses from man and swine. Virology 84: 5162.

T Ichinohe , A Iwasaki and H Hasegawa (2008). Innate sensors of influenza virus: clues to developing better intranasal vaccines. Expert Review of Vaccines 7: 14351445.

AC Jackson , SK SenGupta and JF Smith (1991). Pathogenesis of Venezuelan equine encephalitis virus infection in mice and hamsters. Veterinary Pathology 28: 410418.

H Jin , B Lu , H Zhou , C Ma , J Zhao , CF Yang , G Kemble and H Greenberg (2003). Multiple amino acid residues confer temperature sensitivity to human influenza virus vaccine strains (FluMist) derived from cold-adapted A/Ann Arbor/6/60. Virology 306: 1824.

K Jung and C Chae (2004). Phylogenetic analysis of an H1N2 influenza A virus isolated from a pig in Korea. Brief Report. Archives of Virology 149: 14151422.

SM Kang , JM Song , FS Quan and RW Compans (2009). Influenza vaccines based on virus-like particles. Virus Research 143: 140146.

AI Karasin , J Landgraf , S Swenson , G Erickson , S Goyal , M Woodruff , G Scherba , G Anderson and CW Olsen (2002). Genetic characterization of H1N2 influenza A viruses isolated from pigs throughout the United States. Journal of Clinical Microbiology 40: 10731079.

GM Keating and S Noble (2003). Recombinant hepatitis B vaccine (Engerix-B): a review of its immunogenicity and protective efficacy against hepatitis B. Drugs 63: 10211051.

H Kida , T Ito , J Yasuda , Y Shimizu , C Itakura , KF Shortridge , Y Kawaoka and RG Webster (1994). Potential for transmission of avian influenza viruses to pigs. Journal of General Virology 75: 21832188.

JC King Jr, MM Cox , K Reisinger , J Hedrick , I Graham and P Patriarca (2009). Evaluation of the safety, reactogenicity and immunogenicity of FluBlok trivalent recombinant baculovirus-expressed hemagglutinin influenza vaccine administered intramuscularly to healthy children aged 6–59 months. Vaccine 27: 65896594.

P Kitikoon , D Nilubol , BJ Erickson , BH Janke , TC Hoover , SA Sornsen and EL Thacker (2006). The immune response and maternal antibody interference to a heterologous H1N1 swine influenza virus infection following vaccination. Veterinary Immunology and Immunopathology 112: 117128.

JH Kreijtz , Y Suezer , G van Amerongen , G de Mutsert , BS Schnierle , JM Wood , T Kuiken , RA Fouchier , J Lower , AD Osterhaus , G Sutter and GF Rimmelzwaan (2007). Recombinant modified vaccinia virus Ankara-based vaccine induces protective immunity in mice against infection with influenza virus H5N1. Journal of Infectious Diseases 195: 15981606.

JH Kreijtz , Y Suezer , G de Mutsert , G van Amerongen , A Schwantes , JM van den Brand , RA Fouchier , J Lower , AD Osterhaus , G Sutter and GF Rimmelzwaan (2009a). MVA-based H5N1 vaccine affords cross-clade protection in mice against influenza A/H5N1 viruses at low doses and after single immunization. PLoS One 4: e7790.

JH Kreijtz , Y Suezer , G de Mutsert , JM van den Brand , G van Amerongen , BS Schnierle , T Kuiken , RA Fouchier , J Lower , AD Osterhaus , G Sutter and GF Rimmelzwaan (2009b). Preclinical evaluation of a modified vaccinia virus Ankara (MVA)-based vaccine against influenza A/H5N1 viruses. Vaccine 27: 62966299.

JH Kreijtz , Y Suezer , G de Mutsert , JM van den Brand , G van Amerongen , BS Schnierle , T Kuiken , RA Fouchier , J Lower , AD Osterhaus , G Sutter and GF Rimmelzwaan (2009c). Recombinant modified vaccinia virus Ankara expressing the hemagglutinin gene confers protection against homologous and heterologous H5N1 influenza virus infections in macaques. Journal of Infectious Diseases 199: 405413.

WD Kundin (1970). Hong Kong A-2 influenza virus infection among swine during a human epidemic in Taiwan. Nature 228: 857.

DL Larsen and CW Olsen (2002). Effects of DNA dose, route of vaccination, and coadministration of porcine interleukin-6 DNA on results of DNA vaccination against influenza virus infection in pigs. American Journal of Veterinary Research 63: 653659.

DL Larsen , A Karasin , F Zuckermann and CW Olsen (2000). Systemic and mucosal immune responses to H1N1 influenza virus infection in pigs. Veterinary Microbiology 74: 117131.

P Lekcharoensuk , KM Lager , R Vemulapalli , M Woodruff , AL Vincent and JA Richt (2006). Novel swine influenza virus subtype H3N1, United States. Emerging Infectious Diseases 12: 787794.

P Liljestrom and H Garoff (1991). A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Biotechnology (NY) 9: 13561361.

J Liu , Y Bi , K Qin , G Fu , J Yang , J Peng , G Ma , Q Liu , J Pu and F Tian (2009). Emergence of European avian influenza virus-like H1N1 swine influenza A viruses in China. Journal of Clinical Microbiology 47: 26432646.

MA Liu , W McClements , JB Ulmer , J Shiver and J Donnelly (1997). Immunization of non-human primates with DNA vaccines. Vaccine 15: 909912.

W Ma , M Gramer , K Rossow and KJ Yoon (2006). Isolation and genetic characterization of new reassortant H3N1 swine influenza virus from pigs in the midwestern United States. Journal of Virology 80: 50925096.

W Ma , R Kahn and J Richt (2009a). The pig as a mixing vessel for influenza viruses: Human and veterinary implications. Journal of Molecular and Genetic Medicine 3: 158166.

W Ma , KM Lager , AL Vincent , BH Janke , MR Gramer and JA Richt (2009b). The Role of Swine in the Generation of Novel Influenza Viruses. Zoonoses and Public Health 56: 326337.

HF Maassab (1967). Adaptation and growth characteristics of influenza virus at 25°C. Nature 213: 612614.

K Mahmood , RA Bright , N Mytle , DM Carter , CJ Crevar , JE Achenbach , PM Heaton , TM Tumpey and TM Ross (2008). H5N1 VLP vaccine induced protection in ferrets against lethal challenge with highly pathogenic H5N1 influenza viruses. Vaccine 26: 53935399.

A Masic , LA Babiuk and Y Zhou (2009a). Reverse genetics-generated elastase-dependent swine influenza viruses are attenuated in pigs. Journal of General Virology 90: 375385.

A Masic , JS Booth , GK Mutwiri , LA Babiuk and Y Zhou (2009b). Elastase-dependent live attenuated swine influenza A viruses are immunogenic and confer protection against swine influenza A virus infection in pigs. Journal of Virology 83: 1019810210.

D Matassov , A Cupo and JM Galarza (2007). A novel intranasal virus-like particle (VLP) vaccine designed to protect against the pandemic 1918 influenza A virus (H1N1). Viral Immunology 20: 441452.

JL McAuley , F Hornung , KL Boyd , AM Smith , R McKeon , J Bennink , JW Yewdell and JA McCullers (2007). Expression of the 1918 influenza A virus PB1-F2 enhances the pathogenesis of viral and secondary bacterial pneumonia. Cell Host and Microbe 2: 240249.

J McGill , JW Heusel and KL Legge (2009). Innate immune control and regulation of influenza virus infections. Journal of Leukocyte Biology 86: 803812.

AJ McMichael , FM Gotch and J Rothbard (1986). HLA B37 determines an influenza A virus nucleoprotein epitope recognized by cytotoxic T lymphocytes. Journal of Experimental Medicine 164: 13971406.

H McShane , AA Pathan , CR Sander , SM Keating , SC Gilbert , K Huygen , HA Fletcher and AV Hill (2004). Recombinant modified vaccinia virus Ankara expressing antigen 85A boosts BCG-primed and naturally acquired antimycobacterial immunity in humans. Nature Medicine 10: 12401244.

J Meghrous , W Mahmoud , D Jacob , R Chubet , M Cox and AA Kamen (2009). Development of a simple and high-yielding fed-batch process for the production of influenza vaccines. Vaccine 28: 309316.

M Monteil , MF Le Pottier , AA Ristov , R Cariolet , R L'Hospitalier , B Klonjkowski and M Eloit (2000). Single inoculation of replication-defective adenovirus-vectored vaccines at birth in piglets with maternal antibodies induces high level of antibodies and protection against pseudorabies. Vaccine 18: 17381742.

VS Moorthy , S McConkey , M Roberts , P Gothard , N Arulanantham , P Degano , J Schneider , C Hannan , M Roy , SC Gilbert , TE Peto and AV Hill (2003). Safety of DNA and modified vaccinia virus Ankara vaccines against liver-stage P. falciparum malaria in non-immune volunteers. Vaccine 21: 19952002.

B Moss , MW Carroll , LS Wyatt , JR Bennink , VM Hirsch , S Goldstein , WR Elkins , TR Fuerst , JD Lifson , M Piatak , NP Restifo , W Overwijk , R Chamberlain , SA Rosenberg and G Sutter (1996). Host range restricted, non-replicating vaccinia virus vectors as vaccine candidates. Advances in Experimental Medicine and Biology 397: 713.

T Muller , HJ Batza , H Schluter , FJ Conraths and TC Mettenleiter (2003). Eradication of Aujeszky's disease in Germany. Journal of Veterinary Medicine B, Infectious Diseases and Veterinary Public Health 50: 207213.

RE O'Neill , J Talon and P Palese (1998). The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins. EMBO Journal 17: 288296.

K Okuda , A Ihata , S Watabe , E Okada , T Yamakawa , K Hamajima , J Yang , N Ishii , M Nakazawa , K Ohnari , K Nakajima and KQ Xin (2001). Protective immunity against influenza A virus induced by immunization with DNA plasmid containing influenza M gene. Vaccine 19: 36813691.

CW Olsen (2000). DNA vaccination against influenza viruses: a review with emphasis on equine and swine influenza. Veterinary Microbiology 74: 149164.

CW Olsen (2002). The emergence of novel swine influenza viruses in North America. Virus Research 85: 199210.

CW Olsen , S Carey , L Hinshaw and AI Karasin (2000). Virologic and serologic surveillance for human, swine and avian influenza virus infections among pigs in the north-central United States. Archives of Virology 145: 13991419.

A Ouchi , K Nerome , Y Kanegae , M Ishida , R Nerome , K Hayashi , T Hashimoto , M Kaji , Y Kaji and Y Inaba (1996). Large outbreak of swine influenza in southern Japan caused by reassortant (H1N2) influenza viruses: its epizootic background and characterization of the causative viruses. Journal of General Virology 77: 17511759.

R Paillot , D Hannant , JH Kydd and JM Daly (2006). Vaccination against equine influenza: quid novi? Vaccine 24: 40474061.

LA Perrone , A Ahmad , V Veguilla , X Lu , G Smith , JM Katz , P Pushko and TM Tumpey (2009). Intranasal vaccination with 1918 influenza virus-like particles protects mice and ferrets from lethal 1918 and H5N1 influenza virus challenge. Journal of Virology 83: 57265734.

DC Powers , JE McElhaney , OA Florendo Jr, MC Manning , CM Upshaw , DW Bentley and BE Wilkinson (1997). Humoral and cellular immune responses following vaccination with purified recombinant hemagglutinin from influenza A (H3N2) virus. Journal of Infectious Diseases 175: 342351.

P Pushko , M Parker , GV Ludwig , NL Davis , RE Johnston and JF Smith (1997). Replicon-helper systems from attenuated Venezuelan equine encephalitis virus: expression of heterologous genes in vitro and immunization against heterologous pathogens in vivo. Virology 239: 389401.

P Pushko , TM Tumpey , F Bu , J Knell , R Robinson and G Smith (2005). Influenza virus-like particles comprised of the HA, NA, and M1 proteins of H9N2 influenza virus induce protective immune responses in BALB/c mice. Vaccine 23: 57515759.

X Qi and CP Lu (2006). Genetic characterization of novel reassortant H1N2 influenza A viruses isolated from pigs in southeastern China. Archives of Virology 151: 22892299.

FS Quan , C Huang , RW Compans and SM Kang (2007). Virus-like particle vaccine induces protective immunity against homologous and heterologous strains of influenza virus. Journal of Virology 81: 35143524.

JC Ramirez , MM Gherardi , D Rodriguez and M Esteban (2000). Attenuated modified vaccinia virus Ankara can be used as an immunizing agent under conditions of preexisting immunity to the vector. Journal of Virology 74: 76517655.

JO Rayner , SA Dryga and KI Kamrud (2002). Alphavirus vectors and vaccination. Reviews in Medical Virology 12: 279296.

PA Reay , IM Jones , FM Gotch , AJ McMichael and GG Brownlee (1989). Recognition of the PB1, neuraminidase, and matrix proteins of influenza virus A/NT/60/68 by cytotoxic T lymphocytes. Virology 170: 477485.

KS Reisinger , SL Block , E Lazcano-Ponce , R Samakoses , MT Esser , J Erick , D Puchalski , KE Giacoletti , HL Sings , S Lukac , FB Alvarez and E Barr (2007). Safety and persistent immunogenicity of a quadrivalent human papillomavirus types 6, 11, 16, 18 L1 virus-like particle vaccine in preadolescents and adolescents: a randomized controlled trial. Pediatric Infectious Disease Journal 26: 201209.

JA Richt , P Lekcharoensuk , KM Lager , AL Vincent , CM Loiacono , BH Janke , WH Wu , KJ Yoon , RJ Webby , A Solorzano and A Garcia-Sastre (2006). Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine. Journal of Virology 80: 1100911018.

GF Rimmelzwaan and G Sutter (2009). Candidate influenza vaccines based on recombinant modified vaccinia virus Ankara. Expert Review of Vaccines 8: 447454.

TM Ross , K Mahmood , CJ Crevar , K Schneider-Ohrum , PM Heaton and RA Bright (2009). A trivalent virus-like particle vaccine elicits protective immune responses against seasonal influenza strains in mice and ferrets. PLoS One 4: e6032.

C Scholtissek (1994). Source for influenza pandemics. European Journal of Epidemiology 10: 455458.

C Scholtissek , H Burger , PA Bachmann and C Hannoun (1983). Genetic relatedness of hemagglutinins of the H1 subtype of influenza A viruses isolated from swine and birds. Virology 129: 521523.

S Schultz-Cherry , JK Dybing , NL Davis , C Williamson , DL Suarez , R Johnston and ML Perdue (2000). Influenza virus (A/HK/156/97) hemagglutinin expressed by an alphavirus replicon system protects chickens against lethal infection with Hong Kong-origin H5N1 viruses. Virology 278: 5559.

RE Shope (1931). Swine Influenza : Iii. Filtration Experiments and Etiology. Journal of Experimental Medicine 54: 373385.

W Smith , CH Andrewes and PP Laidlaw (1933). A virus obtained from influenza patients. Lancet 222: 6668.

A Solorzano , RJ Webby , KM Lager , BH Janke , A Garcia-Sastre and JA Richt (2005). Mutations in the NS1 protein of swine influenza virus impair anti-interferon activity and confer attenuation in pigs. Journal of Virology 79: 75357543.

J Stech , H Garn , M Wegmann , R Wagner and HD Klenk (2005). A new approach to an influenza live vaccine: modification of the cleavage site of hemagglutinin. Nature Medicine 11: 683689.

G Sutter , LS Wyatt , PL Foley , JR Bennink and B Moss (1994). A recombinant vector derived from the host range-restricted and highly attenuated MVA strain of vaccinia virus stimulates protective immunity in mice to influenza virus. Vaccine 12: 10321040.

J Szecsi , B Boson , P Johnsson , P Dupeyrot-Lacas , M Matrosovich , HD Klenk , D Klatzmann , V Volchkov and FL Cosset (2006). Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses. Virology Journal 3: 70.

M Tang , JA Harp and RD Wesley (2002). Recombinant adenovirus encoding the HA gene from swine H3N2 influenza virus partially protects mice from challenge with heterologous virus: A/HK/1/68 (H3N2). Archives of Virology 147: 21252141.

E Thacker and B Janke (2008). Swine influenza virus: zoonotic potential and vaccination strategies for the control of avian and swine influenzas. Journal of Infectious Diseases 197 (Suppl. 1): S19S24.

DR Thomsen , KR Marotti , DP Palermo and LE Post (1987). Pseudorabies virus as a live virus vector for expression of foreign genes. Gene 57: 261265.

ZJ Tian , GH Zhou , BL Zheng , HJ Qiu , JQ Ni , HL Yang , XN Yin , SP Hu and GZ Tong (2006). A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge. Veterinary Immunology and Immunopathology 111: 211218.

AR Townsend , AJ McMichael , NP Carter , JA Huddleston and GG Brownlee (1984). Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells. Cell 39: 1325.

HG Townsend , SJ Penner , TC Watts , A Cook , J Bogdan , DM Haines , S Griffin , T Chambers , RE Holland , P Whitaker-Dowling , JS Youngner and RW Sebring (2001). Efficacy of a cold-adapted, intranasal, equine influenza vaccine: challenge trials. Equine Veterinary Journal 33: 637643.

JJ Treanor , RF Betts , GE Smith , EL Anderson , CS Hackett , BE Wilkinson , RB Belshe and DC Powers (1996). Evaluation of a recombinant hemagglutinin expressed in insect cells as an influenza vaccine in young and elderly adults. Journal of Infectious Diseases 173: 14671470.

JJ Treanor , GM Schiff , RB Couch , TR Cate , RC Brady , CM Hay , M Wolff , D She and MM Cox (2006). Dose-related safety and immunogenicity of a trivalent baculovirus-expressed influenza-virus hemagglutinin vaccine in elderly adults. Journal of Infectious Diseases 193: 12231228.

JJ Treanor , GM Schiff , FG Hayden , RC Brady , CM Hay , AL Meyer , J Holden-Wiltse , H Liang , A Gilbert and M Cox (2007). Safety and immunogenicity of a baculovirus-expressed hemagglutinin influenza vaccine: a randomized controlled trial. Journal of the American Medical Association 297: 15771582.

JT van Oirschot , HJ Rziha , PJ Moonen , JM Pol and D van Zaane (1986). Differentiation of serum antibodies from pigs vaccinated or infected with Aujeszky's disease virus by a competitive enzyme immunoassay. Journal of General Virology 67: 11791182.

K Van Reeth , IH Brown and M Pensaert (2000). Isolations of H1N2 influenza A virus from pigs in Belgium. Veterinary Record 146: 588589.

K Van Reeth , V Gregory , A Hay and M Pensaert (2003). Protection against a European H1N2 swine influenza virus in pigs previously infected with H1N1 and/or H3N2 subtypes. Vaccine 21: 13751381.

K Van Reeth , I Brown , S Essen and M Pensaert (2004). Genetic relationships, serological cross-reaction and cross-protection between H1N2 and other influenza A virus subtypes endemic in European pigs. Virus Research 103: 115124.

K Van Reeth , IH Brown , R Durrwald , E Foni , G Labarque , P Lenihan , J Maldonado , I Markowska-Daniel , M Pensaert , Z Pospisil and G Koch (2008). Seroprevalence of H1N1, H3N2 and H1N2 influenza viruses in pigs in seven European countries in 2002–2003. Influenza and Other Respiratory Viruses 2: 99105.

AL Vincent , W Ma , KM Lager , BH Janke , RJ Webby , A Garcia-Sastre and JA Richt (2007). Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine. Vaccine 25: 79998009.

AL Vincent , KM Lager , BH Janke , MR Gramer and JA Richt (2008a). Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccine. Veterinary Microbiology 126: 310323.

AL Vincent , W Ma , KM Lager , BH Janke and JA Richt (2008b). Swine influenza viruses a North American perspective. Advances in Virus Research 72: 127154.

AL Vincent , W Ma , KM Lager , MR Gramer , JA Richt and BH Janke (2009). Characterization of a newly emerged genetic cluster of H1N1 and H1N2 swine influenza virus in the United States. Virus Genes 39: 176185.

AL Vincent , JR Ciacci-Zanella , A Lorusso , PC Gauger , EL Zanella , ME Kehrli Jr, BH Janke and KM Lager (2010a). Efficacy of inactivated swine influenza virus vaccines against the 2009 A/H1N1 influenza virus in pigs. Vaccine 28(15): 27822787.

R Wang , A Song , J Levin , D Dennis , NJ Zhang , H Yoshida , L Koriazova , L Madura , L Shapiro , A Matsumoto , T Mikayama , RT Kubo , S Sarawar , H Cheroutre and S Kato (2008). Therapeutic potential of a fully human monoclonal antibody against influenza A virus M2 protein. Antiviral Research 80: 168177.

Z Wang , C La Rosa , R Maas , H Ly , J Brewer , S Mekhoubad , P Daftarian , J Longmate , WJ Britt and DJ Diamond (2004). Recombinant modified vaccinia virus Ankara expressing a soluble form of glycoprotein B causes durable immunity and neutralizing antibodies against multiple strains of human cytomegalovirus. Journal of Virology 78: 39653976.

RJ Webby , SL Swenson , SL Krauss , PJ Gerrish , SM Goyal and RG Webster (2000). Evolution of swine H3N2 influenza viruses in the United States. Journal of Virology 74: 82438251.

RJ Webby , K Rossow , G Erickson , Y Sims and R Webster (2004). Multiple lineages of antigenically and genetically diverse influenza A virus co-circulate in the United States swine population. Virus Research 103: 6773.

RG Webster (2002). The importance of animal influenza for human disease. Vaccine 20 (Suppl. 2): S16S20.

RG Webster , EF Fynan , JC Santoro and H Robinson (1994). Protection of ferrets against influenza challenge with a DNA vaccine to the haemagglutinin. Vaccine 12: 14951498.

RG Webster , M Peiris , H Chen and Y Guan (2006). H5N1 outbreaks and enzootic influenza. Emerging Infectious Diseases 12: 38.

RD Wesley and KM Lager (2005). Evaluation of a recombinant human adenovirus-5 vaccine administered via needle-free device and intramuscular injection for vaccination of pigs against swine influenza virus. American Journal of Veterinary Research 66: 19431947.

RD Wesley and KM Lager (2006). Overcoming maternal antibody interference by vaccination with human adenovirus 5 recombinant viruses expressing the hemagglutinin and the nucleoprotein of swine influenza virus. Veterinary Microbiology 118: 6775.

RD Wesley , M Tang and KM Lager (2004). Protection of weaned pigs by vaccination with human adenovirus 5 recombinant viruses expressing the hemagglutinin and the nucleoprotein of H3N2 swine influenza virus. Vaccine 22: 34273434.

MR White , M Doss , P Boland , T Tecle and KL Hartshorn (2008). Innate immunity to influenza virus: implications for future therapy. Expert Reviews in Clinical Immunology 497514.

H Xie , TM Liu , X Lu , Z Wu , JA Belser , JM Katz , TM Tumpey and Z Ye (2009). A live attenuated H1N1 M1 mutant provides broad cross-protection against influenza A viruses, including highly pathogenic A/Vietnam/1203/2004, in mice. Journal of Infectious Diseases 200: 18741883.

C Xiong , R Levis , P Shen , S Schlesinger , CM Rice and HV Huang (1989). Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. Science 243: 11881191.

EJ Yager , HJ Dean and DH Fuller (2009). Prospects for developing an effective particle-mediated DNA vaccine against influenza. Expert Review of Vaccines 8: 12051220.

C Yang , L Ye and RW Compans (2008). Protection against filovirus infection: virus-like particle vaccines. Expert Review of Vaccines 7: 333344.

JW Yewdell , JR Bennink , GL Smith and B Moss (1985). Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences USA 82: 17851789.

JS Youngner , P Whitaker-Dowling , TM Chambers , KE Rushlow and R Sebring (2001). Derivation and characterization of a live attenuated equine influenza vaccine virus. American Journal of Veterinary Research 62: 12901294.

H Yu , RH Hua , Q Zhang , TQ Liu , HL Liu , GX Li and GZ Tong (2008). Genetic evolution of swine influenza A (H3N2) viruses in China from 1970 to 2006. Journal of Clinical Microbiology 46: 10671075.

H Yu , PC Zhang , YJ Zhou , GX Li , J Pan , LP Yan , XX Shi , HL Liu and GZ Tong (2009). Isolation and genetic characterization of avian-like H1N1 and novel ressortant H1N2 influenza viruses from pigs in China. Biochemical and Biophysical Research Communication 386: 278283.

Z Yuan , S Zhang , Y Liu , F Zhang , AR Fooks , Q Li and R Hu (2008). A recombinant pseudorabies virus expressing rabies virus glycoprotein: safety and immunogenicity in dogs. Vaccine 26: 13141321.

D Zamarin , MB Ortigoza and P Palese (2006). Influenza A virus PB1-F2 protein contributes to viral pathogenesis in mice. Journal of Virology 80: 79767983.

R Zell , S Motzke , A Krumbholz , P Wutzler , V Herwig and R Durrwald (2008). Novel reassortant of swine influenza H1N2 virus in Germany. Journal of General Virology 89: 271276.

F Zhang , J Chen , F Fang , Y Zhou , J Wu , H Chang , R Zhang , F Wang , X Li , H Wang , G Ma and Z Chen (2005). Maternal immunization with both hemagglutinin- and neuraminidase-expressing DNAs provides an enhanced protection against a lethal influenza virus challenge in infant and adult mice. DNA and Cell Biology 24: 758765.

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Animal Health Research Reviews
  • ISSN: 1466-2523
  • EISSN: 1475-2654
  • URL: /core/journals/animal-health-research-reviews
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