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Comparative testis proteome of cattleyak from different developmental stages

Published online by Cambridge University Press:  27 June 2016

L. Sun ,
T. D. Mipam ,
F. Zhao ,
W. Liu ,
W. Zhao ,
S. Wu ,
C. Xu ,
S. Yu  and
X. Cai
L. Sun
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
T. D. Mipam
Affiliation:
College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, Sichuan, China
F. Zhao
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
W. Liu
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
W. Zhao
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
S. Wu
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
C. Xu
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
S. Yu*
Affiliation:
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
X. Cai*
Affiliation:
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
*
†E-mail: yayushumin@163.com; caixin2323@126.com
†E-mail: yayushumin@163.com; caixin2323@126.com
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Abstract

Cattleyak (hybrid of cattle and yak) exhibit higher capability in adaptability and production than cattle and yak, while the infertility of F1 males greatly restricts the effective utilization of this hybrid and little progress has been made on investigating the mechanisms of the cattleyak infertility. Cattleyak individuals at three development stages (10, 12 and 14-month old) were sampled in this work and the isobaric tag for relative and absolute quantification method was employed to identify differences between their testicular proteomes. The proteomic analysis identified 318 proteins differentially expressed with significance at 12-month stage and 327 at 14-month compared with 10-month stage, respectively. Compared with the testicular proteome from 10-month cattleyak, the gene ontology (GO) annotations of the differentially expressed proteins at 12 months did not indicate significant differences from those at 14 months, which confirmed the histological observation that germ cell reduction was more obvious and spermatogenic arrest may become more serious in 12-month-old cattleyak. On the other hand, 56 differentially expressed proteins were coexpressed at 12 and 14-month stage compared with 10-month stage, in which 32 proteins were upregulated and 24 downregulated. GO analysis revealed that most of the differently expressed proteins were involved in the molecular function of catalytic activity, transporter activity, oxidoreductase activity and protein binding. Further analysis indicated that the differently expressed proteins including testis-expressed protein 101 precursor, RNA-binding motif protein, X chromosome, putative RNA-binding protein 3, heparin-binding proteins, tudor domain-containing protein 1, glutathione S-transferases (GSTA2, GSTP1), heat shock-related 70 kDa protein 2, estradiol 17-β-dehydrogenase11, 2,4-dienoyl-CoA reductase and peroxiredoxin-2 were possibly associated with testis development and spermatogenesis, which could be selected as candidate proteins in future study to examine the mechanisms of cattleyak infertility.

Keywords

cattleyaktestis proteomemale infertilityiTRAQ
Type
Research Article
Information
animal , Volume 11 , Issue 1 , January 2017 , pp. 101 - 111
Copyright
© The Animal Consortium 2016 

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Footnotes

a

These two authors contributed equally to this work.

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