Hostname: page-component-8448b6f56d-sxzjt Total loading time: 0 Render date: 2024-04-19T08:43:54.162Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of the addition of IGF-I and vitamin E to stored boar semen

Published online by Cambridge University Press:  05 December 2012

M. F. B. Mendez ,
M. G. Zangeronimo ,
L. G. P. Rocha ,
B. G. Faria ,
B. A. Pereira ,
C. D. Fernandes ,
B. R. Chaves ,
L. D. S. Murgas  and
R. V. Sousa
M. F. B. Mendez
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
M. G. Zangeronimo*
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
L. G. P. Rocha
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
B. G. Faria
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
B. A. Pereira
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
C. D. Fernandes
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
B. R. Chaves
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
L. D. S. Murgas
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
R. V. Sousa
Affiliation:
Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais 37200-000, Brazil
*
†E-mail: zangeronimo@dmv.ufla.br
Get access

Abstract

The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 15°C, in conjunction with the addition of different amounts of vitamin E (α-tocopherol). Semen samples (n = 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 μg/ml. Immediately after processing and after the doses had been stored at 15°C for 24 or 72 h, samples were warmed at 37°C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 μg/ml of vitamin E to diluted semen reduced (P < 0.01) the malondialdehyde (MDA) production in boar semen stored at 15°C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 37°C. In these conditions, IGF-I also reduced (P < 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced (P = 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 μg/ml of vitamin E reduces the MDA production in boar semen stored at 15°C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 37°C.

Keywords

α-tocopherolantioxidantboar spermatozoainsulin-like growth factor-Ilipid peroxidation
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 5 , May 2013 , pp. 793 - 798
Copyright
Copyright © The Animal Consortium 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alvarez, JG, Storey, BT 1987. Spontaneous lipid peroxidation in rabbit epididymal spermatozoa: its effect on sperm motility. Biology of Reproduction 27, 1102–1108.Google Scholar
Ball, BA, Medina, V, Gravance, CG, Baumber, J 2001. Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5°C. Theriogenology 56, 577–589.Google Scholar
Baronos, S 1971. Seminal carbohydrate in boar and stallion. Journal of Reproduction and Fertility 24, 303–305.Google Scholar
Blom, E 1950. A one-minute live-dead sperm stain by means of eosin–nigrosin. Fertility and Sterility 1, 176–177.Google Scholar
Breininger, E, Beorlegui, NB, O'Flaherty, CM, Beconi, MT 2005. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology 63, 2126–2135.Google Scholar
Cerolini, S, Maldjian, A, Surai, P, Noble, R 2000. Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Animal Reproduction Science 58, 99–111.Google Scholar
Dalvit, GC, Cetica, PD, Beconi, MT 1998. Effect of α-tocopherol and ascorbic acid on bovine in vitro fertilization. Theriogenology 49, 619–627.Google Scholar
Erenpreiss, J, Spano, M, Erenpreisa, A, Bungum, A, Giwercman, A 2006. Sperm chromatin structure and male fertility: biological and clinical aspects. American Journal of Andrology 8, 11–29.Google Scholar
Gancarczyk, M, Kuklińska, M, Sadowska, J, Strzeżek, J, Bilińska, B 2006. Aromatization and antioxidant capacity in the testis of seasonally breeding bank voles: effects of LH, PRL, and IGF-l. Theriogenology 65, 1376–1391.Google Scholar
Henricks, DM, Koubae, AJ, Lackey, BR, Boone, WR, Gray, SL 1998. Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330–337.Google Scholar
Hirai, M, Boersma, A, Hoeflich, A, Wolf, E, Foll, J, Aumuller, TR, Braun, J 2001. Objectively measured sperm motility and sperm head morphometry in boars (Sus scrofa): relation to fertility and seminal plasma growth factors. Journal of Andrology 22, 104–110.Google Scholar
Johannisson, A, Wallgren, M, Rodriguez-Martinez, H 2003. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Animal Reproduction Science 78, 85–98.Google Scholar
Lackey, BR, Gray, SL, Henricks, DM 2002. Measurement of leptin and insulin-like growth factor-I in seminal plasma from different species. Physiology Research 51, 309–311.CrossRefGoogle ScholarPubMed
Lewis, SE, Aitken, RJ 2005. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Research 322, 33–41.Google Scholar
Macpherson, ML, Simmen, RCM, Simmen, FA, Hernandez, J, Sheerin, BR, Varner, DD, Loomis, P, Cadario, ME, Miller, CD, Brinsko, SP, Rigby, S, Blanchard, TL 2002. Insulin-like growth factor-l and insulin-like growth factor binding protein-2 and -5 in equine seminal plasma: association with sperm characteristics and fertility. Biology of Reproduction 67, 648–654.Google Scholar
Minelli, A, Liguori, L, Collodel, G, Lattaioli, P, Castellini, C 2011. Effects of the purified IGF-I complex on the capacitation and acrosome reaction of rabbit spermatozoa. Journal of Experimental Zoology 290, 311–317.CrossRefGoogle Scholar
Nabil-Aziz, MD, Saleh, RA, Sharma, RK, Lewis-Jones, I, Esfandiari, N, Thomas, AJ Jr, Agarwal, A 1999. Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertility and Sterility 2, 484–495.Google Scholar
O'Flaherty, C, Beconi, M, Beorlegui, N 1997. Effect of natural antioxidants, superoxide dismutase and hydrogen peroxide on capacitation of frozen-thawed bull spermatozoa. Andrologia 29, 269–275.Google Scholar
Selvaraju, S, Reddy, IJ, Nandi, S, Rao, SBN, Ravindra, JP 2009. Influence of IGF-I on buffalo (Bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro. Animal Reproduction Science 113, 60–70.Google Scholar
Silva, DM, Zangeronimo, MG, Murgas, LDS, Rocha, LGP, Chaves, BR, Pereira, BA, Cunha, ECP 2011. Addition of IGF-l to storage-cooled boar semen and its effects on sperm quality. Growth Hormone & IGF Research 21, 325–330.Google Scholar
Wolf, R, Wolf, D, Ruocco, V 1998. Vitamin E: the radical protector. Journal of the European Academy of Dermatology and Venereology 10, 103–117.Google Scholar