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Effects of feed additives on the development on the ileal bacterial community of the broiler chicken

  • J. Lu (a1), C. Hofacre (a1), F. Smith (a1) and M. D. Lee (a1)

Intensifying concerns about the use of antimicrobials in meat and poultry production has enhanced interest in the application of prebiotics, probiotics and enzymes to enhance growth and prevent disease in food animals. Growth-promoting antibiotics enhance growth of animals by reducing the load of bacteria in the intestine, by reducing colonization by intestinal pathogens or by enhancing the growth and/or metabolism of beneficial bacteria in the intestine. Recently, molecular ecology, utilizing DNA-sequence heterogeneity of the 16S rRNA gene, has revealed a surprising diversity of uncharacterized bacteria inhabiting this ecosystem. We used this approach to determine the effect of growth-promoting antibiotics on the development and composition of the ileal bacterial community. Pairwise comparisons, correspondence analysis and community diversity indices revealed significant differences among the treatments (bacitracin/virginiamycin or monensin) and controls. Antibiotics reduced the diversity of the ileal bacterial community and induced communities rich in Clostridia throughout the life of the broiler chicken. These results indicate that some bacterial species, such as lactobacilli, were suppressed and also suggest that many intestinal Clostridia may be non-pathogenic. Future studies should focus on characterizing the important bacterial species needed to stabilize the intestinal microbiota and identifying those commensals that stimulate and enhance development of intestinal function.

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Apajalahti, JHA, Sarkilahti, LK, Maki, BRE, Heikkinen, JP, Nurminen, PH, Holben, WE 1998. Effective recovery of bacterial DNA and percent-guanine-plus cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens. Applied and Environmental Microbiology 64, 40844088.
Brorsen, W, Lehenbauer, T, Ji, D, Connor, J 2002. Economic impacts of banning subtherapeutic use of antibiotics in swine production. Journal of Agricultural and Applied Economics 34, 489500.
Bry, L, Falk, PG, Midtvedt, T, Gordon, JI 1996. A model of host-microbial interactions in an open mammalian ecosystem. Science 273, 13801383.
Callaway, TR, Adams, KA, Russell, JB 1999. The ability of ‘low G + C gram-positive’ ruminal bacteria to resist monensin and counteract potassium depletion. Current Microbiology 39, 226230.
Collins, MD, Lawson, PA, Willems, A, Cordoba, JJ, Fernandez-Garayzabal, J, Garcia, P, Cai, J, Hippe, H, Farrow, JAE 1994. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. International Journal of Systematic Bacteriology 44, 812826.
Committee on Drug Use in Food Animals, Panel on Animal Health, Food Safety, and Public Health, National Research Council 1999. The use of drugs in food animal benefits and risks. National Academy Press, Washington, DC.
Cresci, A, Orpianesi, C, Silvi, S, Mastrandrea, V, Dolara, P 1999. The effect of sucrose or starch-based diet on short-chain fatty acids and faecal microflora in rats. Journal of Applied Microbiology 86, 245250.
Decuypere, J, Henderickx, HK, Vervaeke, I 1973. Influence of nutritional doses of virginiamycin and spiromycin on the quantitative and topographical composition of the gastrointestinal microflora of artificially reared piglets. Zentralblatt fur Bakteriologie 223, 348355.
Elsasser, TH, Kahl, S, Steele, NC, Rumsey, TS 1997. Nutritional modulation of somatotrophic axis-cytokine relationships in cattle: a brief review. Comparative Biochemistry Physiololgy – Part A. Physiology 116, 209211.
Falk, PG, Hooper, LV, Midtvedt, T, Gordon, JI 1998. Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology. Microbiology and Molecular Biology Reviews 62, 11571170.
George, BA, Quarles, CL, Fagerberg, DJ 1982. Virginiamycin effects on controlling necrotic enteritis infection in chickens. Poultry Science 61, 447450.
Henderickx, HK, Vervaeke, IJ, Decuypere, JA, Dierick, NA 1982. Effect of growth promoting agents on the intestinal flora. Journal of Veterinary Medicine 33 (Suppl.), 5663.
Hofacre, CL, Froyman, R, Gautrias, B, George, B, Goodwin, MA, Brown, J 1998. Use of Aviguard and other intestinal bioproducts in experimental Clostridium perfringens-associated necrotizing enteritis in broiler chickens. Avian Diseases 42, 579584.
Hooper, LV, Bry, L, Falk, PG, Gordon, JI 1998. Host-microbial symbiosis in the mammalian intestine: exploring an internal ecosystem. Bioessays 20, 336343.
Karasawa, T, Wang, X, Maegawa, T, Michiwa, Y, Kita, H, Miwa, K, Nakamura, S 2003. Clostridium sordellii phospholipase C: gene cloning and comparison of enzymatic and biological activities with those of Clostridium perfringens and Clostridium bifermentans phospholipase C. Infection and Immunity 71, 641646.
Legendre, P, Legendre, L 1998. Numerical ecology, vol. 1 . Elsevier, Amsterdam, The Netherlands.
Leser, TD, Lindecrona, RH, Jensen, TK, Jensen, BB, Møller, K 2000. Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriae. Applied and Environmental Microbiology 66, 32903296.
Long, JR 1973. Necrotic enteritis in broiler chickens: I. A review of the literature and prevalence of the disease in Ontario. Canadian Journal of Comparative Medicine 37, 302308.
Lu, J, Idris, U, Harmon, BG, Hofacre, C, Maurer, JJ, Lee, MD 2003. Diversity and succession of the intestinal bacterial community of the maturing broiler chicken. Applied and Environmental Microbiology 69, 68166824.
Lu, J, Hofacre, CL, Lee, MD 2006. Emerging technologies in microbial ecology aid in understanding the complex disease necrotic enteritis. Journal of Applied Poultry Research 15, 145153.
Magurran, AE 1988. Ecological Diversity and its Measurement. Princeton University Press, Princeton, NJ, pp. 7–46.
McCaig, AE, Glover, LA, Prosser, JI 1999. Molecular analysis of bacterial community structure and diversity in unimproved and improved upland grass pastures. Applied and Environmental Microbiology 65, 17211730.
Petit, L, Gibert, M, Popoff, MR 1999. Clostridium perfringens: toxinotype and genotype. Trends in Microbiology 7, 104110.
Reid, WM, Kowalski, L, Rice, J 1972. Anticoccidial activity of monensin in floor-pen experiments. Poultry Science 51, 139146.
Rood, JI 1998. Virulence genes of Clostridium perfringens. Annual Review of Microbiology 5, 333360.
Salzman, NH, de Jong, H, Paterson, Y, Harmsen, YJ, Welling, GW, Bos, NA 2002. Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. Microbiology 148, 36513660.
Singleton, DR, Furlong, MA, Rathbun, SL, Whitman, WB 2001. Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samples. Applied and Environmental Microbiology 67, 43744376.
Stahl, DA, Flesher, B, Mansfield, HR, Montgomery, L 1988. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Applied and Environmental Microbiology 54, 10791084.
Stappenbeck, TS, Hooper, LV, Manchester, JK, Wong, MH, Gordon, JI 2002. Laser capture microdissection of mouse intestine: characterizing mRNA and protein expression, and profiling intermediary metabolism in specified cell populations. Methods in Enzymology 356, 167196.
Subcommittee on Poultry Nutrition, National Research Council 1994. Nutrient Requirements of Poultry, 9th revised edition, pp. 3–18, 80–84. National Academy Press, Washington, D.C.
Umesaki, Y, Setoyama, H, Matsumoto, S, Imaoka, A, Itoh, K 1999. Differential roles of segmented filamentous bacteria and Clostridia in development of the intestinal immune system. Infection and Immunity 67, 35043511.
Vervaeke IJ, Decuypere JA, Henderickx HK and Dierick NA 1976. Mode of action of some feed additives. Proceedings of the 4th International Pig Veterinary Society Congress, Ames, Iowa, pp. AA.4.
Vervaeke, IJ, Decuypere, JA, Dierick, NA, Henderickx, HK 1979. Quantitative in vitro evaluation of the energy metabolism influence by virginiamycin and spiramycin used as growth promoters in pig nutrition. Journal of Animal Science 4, 1447.
Wages, DP, Opengart, K 2003. Necrotic Enteritis. In Diseases of poultry, 11th edition (ed. YM Saif), pp. 781785. Iowa State University Press, Ames, Iowa.
Walton, JR 1982. Modes of action of growth promoting agents. Journal of Veterinary Medicine 33 (Suppl.), 7782.
Whittaker, RH 1952. A study of summer foliage insect communities in the Great Smoky Mountains. Ecological Monographs 22, 144.
Wierup, M 2001. The Swedish experience of the 1986 year ban of antimicrobial growth promoters, with special reference to animal health, disease prevention, productivity, and usage of antimicrobials. Microbial Drug Resistance 7, 183190.
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