Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-23T16:36:07.605Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of in ovo feeding of l-arginine on breast muscle growth and protein deposition in post-hatch broilers

Published online by Cambridge University Press:  26 February 2018

L. L. Yu ,
T. Gao ,
M. M. Zhao ,
P. A. Lv ,
L. Zhang ,
J. L. Li ,
Y. Jiang ,
F. Gao  and
G. H. Zhou
L. L. Yu
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
T. Gao
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
M. M. Zhao
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
P. A. Lv
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
L. Zhang
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
J. L. Li
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
Y. Jiang
Affiliation:
Ginling College, Nanjing Normal University, Nanjing 210097, China
F. Gao*
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
G. H. Zhou
Affiliation:
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
*
†E-mail: gaofeng0629@sina.com
Get access

Abstract

In ovo feeding (IOF) of l-arginine (Arg) can affect growth performance of broilers, but the response of IOF of Arg on breast muscle growth is unclear, and the mechanism involved in protein deposition remains unknown. Hense, this experiment was conducted to evaluate the effects of IOF of Arg on breast muscle growth and protein-deposited signalling in post-hatch broilers. A total of 720 fertile eggs were collected from 34-week-old Arbor Acres breeder hens and distributed to three treatments: (1) non-injected control group; (2) 7.5 g/l (w/v) NaCl diluent-injected control group; (3) 0.6 mg Arg/egg solution-injected group. At 17.5 days of incubation, fertile eggs were injected 0.6 ml solutions into the amnion of the injected groups. Upon hatching, 80 male chicks were randomly assigned to eight replicates of 10 birds each and fed ad libitum for 21 days. The results indicated that IOF of Arg increased relative breast muscle weight compared with those of control groups at hatch, 3-, 7- and 21-day post-hatch (P<0.05). In the Arg-injected group, the plasma total protein and albumen concentrations were higher at 7- and 21-day post-hatch than those of control groups (P<0.05). The alanine aminotransferase activity in Arg group was higher at hatch than that of control groups (P<0.05). The levels of triiodothyronine at four time points and thyroxine hormones at hatch, 7- and 21-day post-hatch in Arg group were higher than those of control groups (P<0.05). In addition, IOF of Arg increased the amino acid concentrations of breast muscle at hatch, 7- and 21-day post-hatch (P<0.05). In ovo feeding of Arg also enhanced mammalian target of rapamycin, ribosomal protein S6 kinase-1 and eIF4E-bindingprotein-1 messenger RNA expression levels at hatch compared with those of control groups (P<0.05). It was concluded that IOF of Arg treatment improved breast muscle growth, which might be associated with the enhancement of protein deposition.

Keywords

in ovo administrationamino acidhormonesprotein synthesispoultry
Type
Research Article
Information
animal , Volume 12 , Issue 11 , November 2018 , pp. 2256 - 2263
Copyright
© The Animal Consortium 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bakyaraj, S, Bhanja, SK, Majumdar, S and Dash, B 2012. Modulation of post-hatch growth and immunity through in ovo supplemented nutrients in broiler chickens. Journal of the Science of Food and Agriculture 92, 313–320.Google Scholar
Carroll, B, Maetzel, D, Maddocks, OD, Otten, G, Ratcliff, M, Smith, GR, Dunlop, EA, Passos, JF, Davies, OR and Jaenisch, R 2016. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. Elife 5, e11058.Google Scholar
Chen, W, Lv, YT, Zhang, HX, Ruan, D, Wang, S and Lin, YC 2013. Developmental specificity in skeletal muscle of late-term avian embryos and its potential manipulation. Poultry Science 92, 2754–2764.Google Scholar
Corzo, A, Moran, ET, Hoehler, D and Lemmell, A 2005. Dietary tryptophan need of broiler males from forty-two to fifty-six days of age. Poultry Science 84, 226–231.Google Scholar
Decuypere, E, Van, AP, Van, dGS and Darras, VM 2005. Thyroid hormone availability and activity in avian species: a review. Domestic Animal Endocrinology 29, 63–77.Google Scholar
Deng, H, Zheng, A, Liu, G, Chang, W, Zhang, S and Cai, H 2013. Activation of mammalian target of rapamycin signaling in skeletal muscle of neonatal chicks: effects of dietary leucine and age. Poultry Science 93, 114–121.Google Scholar
Emadi, M, Kaveh, K, Bejo, MH, Ideris, A, Jahanshiri, F, Ivan, M and Alimon, RA 2010. Growth performance and blood parameters as influenced by different levels of dietary arginine in broiler chickens. Journal of Animal and Veterinary Advances 9, 70–74.Google Scholar
Fernandes, JI, Murakami, AE, Martins, EN, Sakamoto, MI and Garcia, ER 2009. Effect of arginine on the development of the pectoralis muscle and the diameter and the protein: deoxyribonucleic acid rate of its skeletal myofibers in broilers. Poultry Science 88, 1399–1406.Google Scholar
Foye, O, Ferket, P and Uni, Z 2007. The effects of in ovo feeding arginine, β-hydroxy-β-methyl-butyrate, and protein on jejunal digestive and absorptive activity in embryonic and neonatal turkey poults. Poultry Science 86, 2343–2349.Google Scholar
Foye, OT, Uni, Z and Ferket, PR 2006a. Effect of in ovo feeding egg white protein, β-hydroxy-β-methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys. Poultry Science 85, 1185–1192.Google Scholar
Foye, OT, Uni, Z, Mcmurtry, JP and Ferket, PR 2006b. The effects of amniotic nutrient administration, ‘in ovo feeding’ of Arginine and/or β-hydroxy-β-methyl butyrate (HMB) on insulin-like growth factors, energy metabolism and growth in turkey poults. International Journal of Poultry Science 5, 309–317.Google Scholar
Gao, T, Zhao, MM, Zhang, L, Li, JL, Yu, LL, Lv, PA, Gao, F and Zhou, GH 2017. Effect of in ovo feeding of L-arginine on the hatchability, growth performance, gastrointestinal hormones, and jejunal digestive and absorptive capacity of posthatch broilers. Journal of Animal Science 95, 3079–3092.Google Scholar
Ham, DJ, Caldow, MK, Lynch, GS and Koopman, R 2014. Arginine protects muscle cells from wasting in vitro in an mTORC1-dependent and NO-independent manner. Amino Acids 46, 2643–2652.Google Scholar
Kim, SW and Wu, G 2004. Dietary arginine supplementation enhances the growth of milk-fed young pigs. Journal of Nutrition 134, 625–630.Google Scholar
Kong, X, Tan, B, Yin, Y, Gao, H, Li, X, Jaeger, LA, Bazer, FW and Wu, G 2012. L-arginine stimulates the mTOR signaling pathway and protein synthesis in porcine trophectoderm cells. Journal of Nutritional Biochemistry 23, 1178–1183.Google Scholar
Kornasio, R, Halevy, O, Kedar, O and Uni, Z 2011. Effect of in ovo feeding and its interaction with timing of first feed on glycogen reserves, muscle growth, and body weight. Poultry Science 90, 1467–1477.Google Scholar
Liu, Y, Li, F, Kong, X, Tan, B, Li, Y, Duan, Y, Blachier, F, Hu, CAA and Yin, Y 2015. Signaling pathways related to protein synthesis and amino acid concentration in pig skeletal muscles depend on the dietary protein level, genotype and developmental stages. PLoS ONE 10, e0138277.Google Scholar
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25, 402–408.Google Scholar
Lu, J, McMurtry, J and Coon, C 2007. Developmental changes of plasma insulin, glucagon, insulin-like growth factors, thyroid hormones, and glucose concentrations in chick embryos and hatched chicks. Poultry Science 86, 673–683.Google Scholar
Ohta, Y, Kidd, MT and Ishibashi, T 2001. Embryo growth and amino acid concentration profiles of broiler breeder eggs, embryos, and chicks after in ovo administration of amino acids. Poultry Science 80, 1430–1436.Google Scholar
Ohta, Y, Tsushima, N, Koide, K, Kidd, M and Ishibashi, T 1999. Effect of amino acid injection in broiler breeder eggs on embryonic growth and hatchability of chicks. Poultry Science 78, 1493–1498.Google Scholar
Reyns, G, Venken, K, de Escobar, GM, Kühn, E and Darras, V 2003. Dynamics and regulation of intracellular thyroid hormone concentrations in embryonic chicken liver, kidney, brain, and blood. General and Comparative Endocrinology 134, 80–87.Google Scholar
Shafey, TM, Mahmoud, AH, Alsobayel, AA and Abouheif, MA 2014. Effects of in ovo administration of amino acids on hatchability and performance of meat chickens. South African Journal of Animal Science 44, 123–130.Google Scholar
Tahmasebi, S and Toghyani, M 2016. Effect of arginine and threonine administered in ovo on digestive organ developments and subsequent growth performance of broiler chickens. Journal of Animal Physiology and Animal Nutrition 100, 947–956.Google Scholar
Tan, B, Yin, Y, Liu, Z, Li, X, Xu, H, Kong, X, Huang, R, Tang, W, Shinzato, I, Smith, SB and Wu, G 2008. Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs. Amino Acids 37, 169–175.Google Scholar
Tangara, M, Chen, W, Xu, J, Huang, FR and Peng, J 2010. Effects of in ovo feeding of carbohydrates and arginine on hatchability, body weight, energy metabolism and perinatal growth in duck embryos and neonates. British Poultry Science 51, 602–608.Google Scholar
Uni, Z, Ferket, PR, Tako, E and Kedar, O 2005. In ovo feeding improves energy status of late-term chicken embryos. Poultry Science 84, 764–770.Google Scholar
Wu, G 2009. Amino acids: metabolism, functions, and nutrition. Amino Acids 37, 1–17.Google Scholar
Wu, G, Bazer, FW, Davis, TA, Kim, SW, Peng, L, Rhoads, JM, Satterfield, MC, Smith, SB, Spencer, TE and Yin, Y 2009. Arginine metabolism and nutrition in growth, health and disease. Amino Acids 37, 153–168.Google Scholar
Yao, K, Yin, Y-L, Chu, W, Liu, Z, Deng, D, Li, T, Huang, R, Zhang, J, Tan, B and Wang, W 2008. Dietary arginine supplementation increases mTOR signaling activity in skeletal muscle of neonatal pigs. Journal of Nutrition 138, 867–872.Google Scholar
Yu, LL, Gao, T, Zhao, MM, Lv, PA, Zhang, L, Li, JL, Jiang, Y, Gao, F and Zhou, GH 2017. In ovo feeding of L-arginine alters energy metabolism in post-hatch broilers. Poultry Science 97, 140–148.Google Scholar
Yuan, C, Ding, Y, He, Q, Azzam, M, Lu, J and Zou, X 2015. L-arginine upregulates the gene expression of target of rapamycin signaling pathway and stimulates protein synthesis in chicken intestinal epithelial cells. Poultry Science 94, 1043–1105.Google Scholar
Zhao, MM, Gao, T, Zhang, L, Li, JL, Lv, PA, Yu, LL, Gao, F and Zhou, GH 2017. Effects of in ovo feeding of creatine pyruvate on the hatchability, growth performance and energy status in embryos and broiler chickens. Animal 11, 1689–1697.Google Scholar
Supplementary material: File

Yu et al. supplementary material

Table S1

Download Yu et al. supplementary material(File)
File 16.1 KB