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Expression of seasonality in Merinos d’Arles ewes of different genotypes at the MT1 melatonin receptor gene

  • J. Teyssier (a1) (a2) (a3), M. Migaud (a4) (a5) (a6) (a7), N. Debus (a1) (a2) (a3), C. Maton (a1) (a2) (a3), E. Tillard (a1) (a2) (a3), B. Malpaux (a4) (a5) (a6) (a7), P. Chemineau (a4) (a5) (a6) (a7) and L. Bodin (a8)...

Spontaneous ovulatory activity (SOA) in spring has been used to study the out-of-season breeding ability of Merinos d’Arles (MA) ewes. Within this breed, an association was found between more intense seasonality and genotype −/− at a MnlI restriction site (allele − for its absence v. + for its presence) in Exon II of the MT1 receptor gene. This study was designed to ascertain whether this association results in a direct effect of the MT1 genotype on the expression of seasonality in MA ewes. In the first year of the study, genotyping of 314 MA ewes at locus MnlI was carried out and resulted in frequencies of 43.0%, 44.9% and 12.1% for genotypes +/+, +/− and −/−, respectively. The SOA of these ewes was determined in early April of two consecutive years by assaying plasma progesterone concentrations in two blood samples taken 9 days apart. Groups of 30 ewes of each homozygous genotype (+/+ and −/−) were identified from this population and their SOA was followed by taking blood samples at regular intervals between January and mid-April of the second and third year of the study. In the second year, groups of ewes were managed together on rangelands, whereas in the third year each group was split into two subgroups given differential feed levels. The results clearly showed that genotype had no significant effect on SOA during the 2- to 3-month period preceding the introduction of rams for spring mating. In the second year of the study, in which the experimental procedure allowed a fair comparison of the fertility of ewes in spring mating, fertility was similar for both genotypes. The reciprocity of the association was not demonstrated and the MnlI polymorphic site could not be used as a genetic marker of selection for out-of-season breeding ability, at least not in the MA breed. The percentage of cycling ewes significantly decreased between January and April, and older ewes (5 or 6 years old depending on the year of the study) were more cyclic than younger ones (2 and 3 years old, respectively). The differential feeding level of ewes from early February did not significantly affect their SOA during the time period studied in the third year of the study.

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Avdi, M, Banos, G, Kouttos, A, Bodin, L, Chemineau, P 2003. Sources of variation and genetic profile of spontaneous, out-of-season ovulatory activity in the Chios sheep. Genetics Selection Evolution 35, 6576.
Beltran de Heredia, I, Urgate, E, Malpaux, B, Canepa, S, Bodin, L 2002. Genetic aspects of out of season breeding ability in Latxa sheep. In Proceedings of the 7th World Congress on Genetics Applied to Livestock Production, vol. 30. pp. 717720. Institut National de Recherche Agronomique (INRA), Castanet-Tolosan, France.
Chu, MX, Ji, CL, Chen, GH 2003. Association between PCR-RFLP of melatonin receptor 1a gene and high prolificacy in Small Tail Han sheep. Asian-Australasian Journal of Animal Sciences 16, 17011704.
Diggle, PJ, Heagerty, P, Liang, KY, Zeger, SL 2002. Analysis of longitudinal data, 2nd edition. Oxford University Press, New York, USA.
Hanocq, E, Bodin, L, Thimonier, J, Teyssier, J, Malpaux, B, Chemineau, P 1999. Genetics parameters of spontaneous spring ovulatory activity in Merinos d’Arles sheep. Genetics Selection Evolution 31, 7790.
Hernandez, X, Bodin, L, Chesneau, D, Guillaume, D, Chemineau, P, Malpaux, B, Migaud, M 2005. Relationship between MT1 melatonin receptor gene polymorphism and seasonal physiological responses in Ile-de-France ewes. Reproduction Nutrition Development 45, 151162.
Hosmer, DW, Lemeshow, S 2000. Applied logistic regression, 2nd edition. John Wiley & Sons, Inc., New York, USA.
Jarrige, R 1988. Alimentation des bovins, ovins et caprins, 1st edition. Institut National de Recherche Agronomique (INRA), Paris, France.
Khaldi, G 1984. Variations saisonnières de l’activité ovarienne, du comportement d’oestrus et de la durée de l’anoestrus post-partum des femelles ovines de race Barbarine: influences du niveau alimentaire et de la présence du mâle. PhD thesis, Montpellier University, France.
Kleemann, DO, Walker, SK 2005. Fertility in South Australian commercial Merino flocks: relationships between reproductive traits and environmental cues. Theriogenology 63, 24162433.
Malpaux, B, Migaud, M, Tricoire, H, Chemineau, P 2001. Biology of mammalian photoperiodism and the critical role of the pineal gland and melatonin. Journal of Biological Rhythms 16, 336347.
Mateescu, RG, Lunsford, AK, Thonney, ML 2009. Association between melatonin receptor 1A gene polymorphism and reproductive performance in Dorset ewes. Journal of Animal Science 87, 24852488.
Messer, LA, Wang, L, Tuggle, CK, Yerle, M, Chardon, P, Pomp, D, Womack, JE, Barendse, W, Crawford, AM, Notter, DR, Rothschild, MF 1997. Mapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep, and cattle. Mammalian Genome 8, 368370.
Notter, DR, Cockett, NE, Hadfield, TS 2003. Evaluation of melatonin receptor 1a as a candidate gene influencing reproduction in an autumn-lambing sheep flock. Journal of Animal Science 81, 912917.
Notter, DR, Cockett, NE 2005. Opportunities for detection and use of QTL influencing seasonal reproduction in sheep: a review. Genetics Selection Evolution 37 (suppl. 1), S39S53.
Pelletier, J, Bodin, L, Hanocq, E, Malpaux, B, Teyssier, J, Thimonier, J, Chemineau, P 2000. Association between expression of reproductive seasonality and alleles of the gene for Mel1a receptor in the ewe. Biology of Reproduction 62, 10961101.
R Development Core Team 2007. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
Russel, AJF, Doney, JM, Gunn, RG 1969. Subjective assessment of body fat in live sheep. The Journal of Agricultural Science 72, 451454.
Terqui, M, Thimonier, J 1974. Nouvelle méthode radioimmunologique rapide pour l’estimation du niveau de progestérone plasmatique. Application pour le diagnostic précoce de gestation chez la brebis et la chèvre. C. R. de l’Académie des sciences, Paris, Série D 279, 11091112.
Teyssier, J, Canepa, S, Chemineau, P, Malpaux, B, Migaud, M, Bodin, L 2002. Preliminary results on selection lines for spontaneous spring ovulatory activity in Merinos d’Arles sheep. In Proceedings of the 7th World Congress on Genetics Applied to Livestock Production, vol. 30, pp. 685688. Institut National de Recherche Agronomique (INRA), Castanet-Tolosan, France.
Teyssier, J, Migaud, M, Debus, N, Maton, C, Bouquet, PM, Bocquier, F, Chemineau, P, Malpaux, B, Bodin, L 2006. Expression of seasonality in the Merinos d’Arles ewe according to the genotype of the melatonin receptor (MT1) gene and the feeding level. In 8th World Congress on Genetics Applied to Livestock Production, CD-ROM comm. 11–14. Sociedade Brasileira de Melhoramento Animal (SBMA), Belo Horizonte, Brazil.
Thimonier, J 2000. Progesterone level analysis for the determination of the physiological status of female farm animals (in French). Productions Animales 13, 177183.
Trecherel, E 2007. Relationship between MT1 melatonin receptor polymorphism and seasonality of reproduction in sheep through genotyping and functional studies (in French). PhD thesis, Tours University France.
Ugarte, E, Beltran de Heredia, I, Bodin, L 2007. Exploring the relationship between the polymorphism of the melatonin receptor gene and the variability of sheep seasonality. 58th Annual EAAP Meeting, Dublin, Ireland.
Yule, GU 1912. On the methods of measuring association between two attributes, (with discussion). Journal of the Royal Statistical Society 75, 579652.
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